Street vitality is associated with a comfortable human-based public environment and urban sustainability. In most current studies, street vitality is assessed considering single or multi factors; however, the impact of time dimension is ignored. This study selects nine different year-built streets in old, main, and new urban areas, in Nanjing, China, proposes a framework to assess street vitality considering the different time dimensions and selects the following factors: street form, including building density, continuity, and height-width; street business type, including store density, function density, and permeation rate; and street accessibility, including location, the number of entrances/exits, transportation, and walkability. After calculating the values of the subfactors, a ranking method was applied to assign the ranking of impact of all factors for a comprehensive analysis. The results showed that Pipa Street, Wufu Street in a main urban area, and Hongmiao Street had the highest street vitality and the highest rankings of almost all the factors. Street vitality in different periods demonstrated that street vitality in new urban areas is lower compared with old and main urban areas.features to present the vitality of urban streets. Jacobs [10] also attempted to find desirable street forms to provide a reference for the design of such urban spaces.Extensive research studies have investigated the correlations between transportation, road type, car parking, pedestrian environment, and street vitality, respectively. It is vital to design suitable streets by creating a safe and comfortable environment [11][12][13]. Boer [14] suggested reducing the speed limit of cars to give pedestrians priority. Ikioda [15] explored the impact of road construction on market and street traders in urban environments, and Calanis et al. [16] analyzed the road safety of people on the street while considering urban road types and traffic. For street car parking, Ajeng and Gim [17] studied on-street car parking issues and analyzed the differentiation between car parking duration and demand on streets in Yogyakarta City, Indonesia. Park et al. [18] analyzed the impact of on-street car parking on the thermal environment of streets for pedestrians according to Korea's green parking project. For the pedestrian environment, Kang also applied multilevel regression models to identify the positive effects of street facilities, such as stores, cultural issues, local street networks on walking volume, and so forth [19].The built environment is also important for street vitality [20,21]. Park et al. [22] applied a multilayer mean radiant temperature model for pedestrians on streets according to the built environment. Sung et al. [23] identified the association of the physical environment with the activity on streets in Seoul City. Lee et al. [24] investigated urban heat island environment cooling with the use of water spray systems in urban street canyons. Lee [25] analyzed the thermal characteristics of streets using a thermal imaging came...