Open-Spaced Ridged Hydrogel Scaffolds Containing TiO2-Self-Assembled Monolayer of Phosphonates Promote Regeneration and Recovery Following Spinal Cord Injury

Siddiqui, Ahad M.; Thiele, Frederic; Stewart, Rachel; Rangnick, Simone; Weiss, Georgina J.; Chen, Bingkun K.; Silvernail, Jodi L.; Strickland, Tammy; Nesbitt, Jarred J.; Lim, Kelly; Schwarzbauer, Jean E.; Schwartz, Jeffrey; Yaszemski, Michael J.; Windebank, Anthony J.; Madigan, Nicolas N.

doi:10.3390/ijms241210250

Cited by 3 publications

(1 citation statement)

References 103 publications

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“…In the context of SCI, the combination of stem and progenitor cell transplantation with biomaterial scaffolds shows promising potential as an alternative treatment. This approach can recover lost local cells through the correct differentiation of progenitor and stem cells while providing essential support for neural network formation and guiding axon growth [38][39][40]. However, the interaction between these biomaterials and the local and transplanted cells should be ensured [41].…”

Section: Introductionmentioning

confidence: 99%

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

da Silva,

Bobotis,

Correia

et al. 2023

IJMS

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Tissue engineering for spinal cord injury (SCI) remains a complex and challenging task. Biomaterial scaffolds have been suggested as a potential solution for supporting cell survival and differentiation at the injury site. However, different biomaterials display multiple properties that significantly impact neural tissue at a cellular level. Here, we evaluated the behavior of different cell lines seeded on chitosan (CHI), poly (ε-caprolactone) (PCL), and poly (L-lactic acid) (PLLA) scaffolds. We demonstrated that the surface properties of a material play a crucial role in cell morphology and differentiation. While the direct contact of a polymer with the cells did not cause cytotoxicity or inhibit the spread of neural progenitor cells derived from neurospheres (NPCdn), neonatal rat spinal cord cells (SCC) and NPCdn only attached and matured on PCL and PLLA surfaces. Scanning electron microscopy and computational analysis suggested that cells attached to the material’s surface emerged into distinct morphological populations. Flow cytometry revealed a higher differentiation of neural progenitor cells derived from human induced pluripotent stem cells (hiPSC-NPC) into glial cells on all biomaterials. Immunofluorescence assays demonstrated that PCL and PLLA guided neuronal differentiation and network development in SCC. Our data emphasize the importance of selecting appropriate biomaterials for tissue engineering in SCI treatment.

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Section: Introductionmentioning

confidence: 99%

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

da Silva,

Bobotis,

Correia

et al. 2023

IJMS

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Artificial Intelligence (AI): A Potential Game Changer in Regenerative Orthopedics—A Scoping Review

Vaishya,

Dhall,

Vaish

2024

JOIO

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Gelatin Methacrylic Acid Hydrogel-Based Nerve Growth Factors Enhances Neural Stem Cell Growth and Differentiation to Promote Repair of Spinal Cord Injury

Shen,

Wang,

et al. 2024

IJN

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Background The challenge in treating irreversible nerve tissue damage has resulted in suboptimal outcomes for spinal cord injuries (SCI), underscoring the critical need for innovative treatment strategies to offer hope to patients. Methods In this study, gelatin methacrylic acid hydrogel scaffolds loaded with nerve growth factors (GMNF) were prepared and used to verify the performance of SCI. The physicochemical and biological properties of the GMNF were tested. The effect of GMNF on activity of neuronal progenitor cells (NPCs) was investigated in vitro. Histological staining and motor ability was carried out to assess the ability of SCI repair in SCI animal models. Results Achieving nerve growth factors sustained release, GMNF had good biocompatibility and could effectively penetrate into the cells with good targeting permeability. GMNF could better enhance the activity of NPCs and promote their directional differentiation into mature neuronal cells in vitro, which could exert a good neural repair function. In vivo, SCI mice treated with GMNF recovered their motor abilities more effectively and showed better wound healing by macroscopic observation of the coronal surface of their SCI area. Meanwhile, the immunohistochemistry demonstrated that the GMNF scaffolds effectively promoted SCI repair by better promoting the colonization and proliferation of neural stem cells (NSCs) in the SCI region and targeted differentiation into mature neurons. Conclusion The application of GMNF composite scaffolds shows great potential in SCI treatment, which are anticipated to be a potential therapeutic bioactive material for clinical application in repairing SCI in the future.

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Open-Spaced Ridged Hydrogel Scaffolds Containing TiO2-Self-Assembled Monolayer of Phosphonates Promote Regeneration and Recovery Following Spinal Cord Injury

Cited by 3 publications

References 103 publications

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

The Impact of Biomaterial Surface Properties on Engineering Neural Tissue for Spinal Cord Regeneration

Artificial Intelligence (AI): A Potential Game Changer in Regenerative Orthopedics—A Scoping Review

Gelatin Methacrylic Acid Hydrogel-Based Nerve Growth Factors Enhances Neural Stem Cell Growth and Differentiation to Promote Repair of Spinal Cord Injury

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