Opening of endothelial cell–cell contacts due to sonoporation

Beekers, Inés; Vegter, Merel; Lattwein, Kirby R.; Mastik, Frits; Beurskens, Robert; Steen, Antonius F.W. van der; Jong, Nico de; Verweij, Martin D.; Kooiman, Klazina

doi:10.1016/j.jconrel.2020.03.038

Cited by 62 publications

(78 citation statements)

References 56 publications

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“…[ 44 ] Importantly, many studies demonstrate that ultrasound sonoporation can improve drug diffusion into solid tumors by enhancing vascular permeability. [ 45 ] In addition, ultrasound cavitation effects, such as jet formation, shock waves, and microstreaming, can increase cell membrane permeability, resulting in enhanced cellular uptake of nanoparticles. [ 46 ] Therefore, the combination of MSCs and SDT may offer a potent approach for overcoming tumor treatment limitations.…”

Section: Figurementioning

confidence: 99%

Mesenchymal Stem Cells Functionalized Sonodynamic Treatment for Improving Therapeutic Efficacy and Compliance of Orthotopic Oral Cancer

Sun

Zhang

et al. 2020

Advanced Materials

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Despite multiple treatment options being available, many critical challenges are still ongoing in the treatment of oral squamous cell carcinoma (OSCC). Particularly, the major hurdle is to avoid facial disfigurement and oral function disability during treatment. Herein, nanoengineered mesenchymal stem cells (MSCs) are developed as a supersonosensitizer, named M/LPV/O2, for improving nondestructive sonodynamic therapy (SDT) against OSCC along with good therapeutic compliance. M/LPV/O2 is composed of an MSCs membrane functionalized liposomal formulation of oxygen‐loading perfluorocarbon and sonosensitizer verteporfin (M/LPV/O2), which can not only increase circulation and targeting efficacy but also supply oxygen to overcome tumor‐hypoxia‐associated resistance in SDT, resulting in enhanced therapeutic outcomes in vitro and in vivo. It is identified that M/LPV/O2 effectively stimulates the generation of reactive oxygen species even in hypoxic conditions, and consequently tremendously induces cancer cell death. In addition, M/LPV/O2 displays good tumor accumulation and penetration under ultrasound stimulation, and efficiently induces tumor inhibition and even abrogation, leading to prolonged survival of tumor‐bearing mice. Importantly, M/LPV/O2‐based SDT exhibits minimal systemic adverse effects and successfully maintains oral functions with no facial tissue damage. Therefore, these studies provide a promising therapeutic strategy for OSCC, which has a potential to enhance life quality and compliance after treatment.

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Section: Figurementioning

confidence: 99%

Mesenchymal Stem Cells Functionalized Sonodynamic Treatment for Improving Therapeutic Efficacy and Compliance of Orthotopic Oral Cancer

Sun

Zhang

et al. 2020

Advanced Materials

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“…It was estimated that the pore size may vary from 1 nm to several micrometers 11 . Pore resealing occurs very fast and it may take from a few up to 120 s 12 . On the other hand, high intensity focused US may cause an irreversible sonoporation 13 .…”

mentioning

confidence: 99%

Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures

Paškevičiūtė

Januškevičienė

Sakalauskienė

et al. 2020

Sci Rep

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The aim of our study was to evaluate the influence of low-intensity pulsed US on the delivery of doxorubicin (DOX) into MDA-MB-231 triple-negative breast cancer and A549 non-small cell lung cancer cell 2D and 3D cultures. US with pulse repetition frequency of 10 Hz and 1 MHz center frequency was generated with peak negative pressure of 0.5 MPa and 50% duty cycle. SonoVue microbubbles were used. Spheroids were formed using 3D Bioprinting method. DOX delivery in 2D and 3D cultures was assessed using fluorescence microscopy. US without the addition of microbubbles did not enhance the penetration of DOX into monolayer-cultured cells and tumor spheroids. In the presence of microbubbles US improved the delivery of DOX into the edge end middle zones of A549 and MDA-MB-231 spheroids. Application of low-intensity pulsed US in combination with microbubbles may be a promising approach to enhance the delivery of DOX into tumor spheroids.

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“…Both stable and inertial cavitation can cause the formation of current-conducting pores detectable by the patch-clamp technique [ 72 ]. During membrane poration, membrane-impermeable molecules (such as propidium iodide, often used as a model drug) have been observed to gain access to the cell cytoplasm [ 73 , 74 ]. In vitro, pores may or may not reseal, the latter leading to cell death [ 75 ].…”

Section: Ultrasound and Microbubble-induced Atp Release For Enhancing Cancer Immunotherapymentioning

confidence: 99%

Lytic Release of Cellular ATP: Physiological Relevance and Therapeutic Applications

Grygorczyk

Boudreault

Ponomarchuk

et al. 2021

Life

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The lytic release of ATP due to cell and tissue injury constitutes an important source of extracellular nucleotides and may have physiological and pathophysiological roles by triggering purinergic signalling pathways. In the lungs, extracellular ATP can have protective effects by stimulating surfactant and mucus secretion. However, excessive extracellular ATP levels, such as observed in ventilator-induced lung injury, act as a danger-associated signal that activates NLRP3 inflammasome contributing to lung damage. Here, we discuss examples of lytic release that we have identified in our studies using real-time luciferin-luciferase luminescence imaging of extracellular ATP. In alveolar A549 cells, hypotonic shock-induced ATP release shows rapid lytic and slow-rising non-lytic components. Lytic release originates from the lysis of single fragile cells that could be seen as distinct spikes of ATP-dependent luminescence, but under physiological conditions, its contribution is minimal <1% of total release. By contrast, ATP release from red blood cells results primarily from hemolysis, a physiological mechanism contributing to the regulation of local blood flow in response to tissue hypoxia, mechanical stimulation and temperature changes. Lytic release of cellular ATP may have therapeutic applications, as exemplified by the use of ultrasound and microbubble-stimulated release for enhancing cancer immunotherapy in vivo.

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Opening of endothelial cell–cell contacts due to sonoporation

Cited by 62 publications

References 56 publications

Mesenchymal Stem Cells Functionalized Sonodynamic Treatment for Improving Therapeutic Efficacy and Compliance of Orthotopic Oral Cancer

Mesenchymal Stem Cells Functionalized Sonodynamic Treatment for Improving Therapeutic Efficacy and Compliance of Orthotopic Oral Cancer

Evaluation of low-intensity pulsed ultrasound on doxorubicin delivery in 2D and 3D cancer cell cultures

Lytic Release of Cellular ATP: Physiological Relevance and Therapeutic Applications

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