to analyze the perception of nurses about the systematization of nursing care in the perspectiveof professional autonomy. Methods: a descriptive and exploratory research, carried out with 24 nurses atfive inpatient clinics of a large-scale public teaching hospital, through a structured questionnaire evaluatedby experts. Data were analyzed using descriptive statistics. Results: nurses claimed that the systematizationof nursing care promotes autonomy, emphasizing that the pursuit of knowledge for the development ofsystematization of nursing care is supported by an exchange of experiences among colleagues. Conclusion:applying the systematization of nursing care generates autonomy, demonstrating that nurses become more aptin developing it, thereby resulting in increased professional autonomy.