Ophiopogonin D ameliorates non‑alcoholic fatty liver disease in high‑fat diet‑induced obese mice by improving lipid metabolism, oxidative stress and inflammatory response

Abstract

Lipid metabolic disorders, oxidative stress and inflammation in the liver are key steps in the progression of non-alcoholic fatty liver disease (NAFLD). Ophiopogonin D (OP-D), the main active ingredient of Ophiopogon japonicus , exhibits several pharmacological activities such as antioxidant and anti-inflammatory activities. Therefore, the current study aimed to explore the role of OP-D in NAFLD in a high-fat diet (HFD)-induced obesity mouse model. To investigate the effect of OP-D on NAFLD in vivo , a NAFLD mouse model was established following feeding mice with HFD, then the mice were randomly treated with HFD or HFD + OP-D for 4 weeks. Subsequently, primary mouse hepatocytes were isolated, and enzyme-linked immunosorbent assay, reverse transcription-quantitative PCR western blotting and immunofluorescence analysis were used for assessment to explore the direct effect of OP-D in vitro . The results of the present study indicated that OP-D could ameliorate NAFLD in HFD-induced obese mice by regulating lipid metabolism and antioxidant and anti-inflammatory responses. Additionally, OP-D treatment decreased lipogenesis and inflammation levels in vitro , suggesting that the NF-κB signaling pathway may be involved in the beneficial effects of OP-D on NAFLD.

The Combination of Remnant Cholesterol and Waist Circumference Offers Predictive Value for Nonalcoholic Fatty Liver Disease: A Cross-Sectional Study from a Medical Examination Center

“…Previous research suggests that Ophiopogonin D (OP-D), the main active component of Ophiopogon japonicus , can ameliorate NAFLD in obese mice by modulating lipid metabolism. 20 Furthermore, inhibition of lymphocyte antigen 6 family member D (Ly6d), a primary regulator of hepatic steatosis, can also reduce hepatic steatosis in a diet-induced mouse model of NAFLD. 21 In terms of abnormal lipid metabolism, the RC index, as a composite measure, maybe both more effective and reliable as a predictive indicator of NAFLD compared to relying on a single lipid index.…”

Ophiopogonin D: review of pharmacological activity

“…In vitro , OP-D treatment lowered the levels of inflammation and lipogenesis. One possible explanation for OP-D’s beneficial effects on NAFLD was the NF-κB signaling pathway ( Huang et al, 2023 ).…”