Ophthalmic Nanosystems with Antioxidants for the Prevention and Treatment of Eye Diseases

Dinte, Elena; Voștinaru, Oliviu; Samoilă, O; Sevastre, Bogdan; Bodoki, Ede

doi:10.3390/coatings10010036

Cited by 11 publications

(6 citation statements)

References 66 publications

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“…In fact, iron, similar to ROS, functions as an inducible stress intracellular messenger that serves to regulate cell death responses. Iron chelators can act as powerful antioxidants [128]; Eid et al communicated in 2017 that intracellular iron chelators were found to function as ROS scavengers [52].…”

Section: Non-enzymatic Antioxidant Effects Of Bioflavonoids From Elde...mentioning

confidence: 99%

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

et al. 2022

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The use of gentamicin (GM) is limited due to its nephrotoxicity mediated by oxidative stress. This study aimed to evaluate the capacity of a flavonoid-rich extract of Sambucus nigra L. elderflower (SN) to inhibit lipoperoxidation in GM-induced nephrotoxicity. The HPLC analysis of the SN extract recorded high contents of rutin (463.2 ± 0.0 mg mL−1), epicatechin (9.0 ± 1.1 µg mL−1), and ferulic (1.5 ± 0.3 µg mL−1) and caffeic acid (3.6 ± 0.1 µg mL−1). Thirty-two Wistar male rats were randomized into four groups: a control group (C) (no treatment), GM group (100 mg kg−1 bw day−1 GM), GM+SN group (100 mg kg−1 bw day−1 GM and 1 mL SN extract day−1), and SN group (1 mL SN extract day−1). Lipid peroxidation, evaluated by malondialdehyde (MDA), and antioxidant enzymes activity—superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX)—were recorded in renal tissue after ten days of experimental treatment. The MDA level was significantly higher in the GM group compared to the control group (p < 0.0001), and was significantly reduced by SN in the GM+SN group compared to the GM group (p = 0.021). SN extract failed to improve SOD, CAT, and GPX activity in the GM+SN group compared to the GM group (p > 0.05), and its action was most probably due to the ability of flavonoids (rutin, epicatechin) and ferulic and caffeic acids to inhibit synthesis and neutralize reactive species, to reduce the redox-active iron pool, and to inhibit lipid peroxidation. In this study, we propose an innovative method for counteracting GM nephrotoxicity with a high efficiency and low cost, but with the disadvantage of the multifactorial environmental variability of the content of SN extracts.

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Section: Non-enzymatic Antioxidant Effects Of Bioflavonoids From Elde...mentioning

confidence: 99%

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

et al. 2022

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“…In parallel to the design of surfaces that may prevent the adhesion of bacteria, the incorporation into the lenses of natural compounds that can act as biofilm inhibitors is gaining attention. In this regard, resveratrol (trans-3,5,4′-trihydroxystilbene) ( Figure 1 ), which is a natural antioxidant polyphenol [ 21 ] with good ocular tolerance [ 22 , 23 ], has been reported to interfere in vitro with the bacterial communication process known as quorum sensing (QS) and to inhibit biofilm formation by clinically relevant bacteria such as S. aureus , P. aeruginosa and Vibrio cholerae [ 24 , 25 , 26 , 27 ]. The interference in QS processes may increase the susceptibility to antibiotics and downregulate natural, industrial and clinical biofilms [ 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…This work relies on the hypothesis that the design of hydrogels containing MPC moieties and incorporating resveratrol may exhibit a synergism in terms of the prevention of biofilm formation on CLs, while resveratrol may also lead to other beneficial effects related to its antioxidant capability. Some eye diseases caused by oxidative stress mechanisms, such as age-related macular degeneration, cataracts and glaucoma, may be treated or prevented with antioxidants [ 23 , 33 ]. To the best of our knowledge, loading of resveratrol in CLs has not been tested before.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol-Loaded Hydrogel Contact Lenses with Antioxidant and Antibiofilm Performance

et al. 2021

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Contact lenses (CLs) are prone to biofilm formation, which may cause severe ocular infections. Since the use of antibiotics is associated with resistance concerns, here, two alternative strategies were evaluated to endow CLs with antibiofilm features: copolymerization with the antifouling monomer 2-methacryloyloxyethyl phosphorylcholine (MPC) and loading of the antioxidant resveratrol with known antibacterial activity. MPC has, so far, been used to increase water retention on the CL surface (Proclear® 1 day CLs). Both poly(hydroxyethyl methacrylate) (HEMA) and silicone hydrogels were prepared with MPC covering a wide range of concentrations (from 0 to 101 mM). All hydrogels showed physical properties adequate for CLs and successfully passed the hen's egg-chorioallantoic membrane (HET-CAM) test. Silicone hydrogels had stronger affinity for resveratrol, with higher loading and a slower release rate. Ex vivo cornea and sclera permeability tests revealed that resveratrol released from the hydrogels readily accumulated in both tissues but did not cross through. The antibiofilm tests against Pseudomonas aeruginosa and Staphylococcus aureus evidenced that, in general, resveratrol decreased biofilm formation, which correlated with its concentration-dependent antibacterial capability. Preferential adsorption of lysozyme, compared to albumin, might also contribute to the antimicrobial activity. In addition, importantly, the loading of resveratrol in the hydrogels preserved the antioxidant activity, even against photodegradation. Overall, the designed hydrogels can host therapeutically relevant amounts of resveratrol to be sustainedly released on the eye, providing antibiofilm and antioxidant performance.

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“… 16 , 51 , 52 Nevertheless, the use of antioxidants to prevent and support the present pharmacological treatments is still being vigorously pursued as novel methods of drug delivery to the eye such as nanoformulations become available. 54 However, additional studies are needed to define the antioxidant class and formulations beneficial for AMD.…”

Section: Discussionmentioning

confidence: 99%

Using Advanced Bioinformatics Tools to Identify Novel Therapeutic Candidates for Age-Related Macular Degeneration

Nadeem

Xie

et al. 2022

Trans. Vis. Sci. Tech.

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Purpose Age-related macular degeneration (AMD) is the most common cause of aging-related blindness in the developing world. Although medications can slow progressive wet AMD, currently, no drugs to treat dry-AMD are available. We use a systems or in silico biology analysis to identify chemicals and drugs approved by the Food and Drug Administration for other indications that can be used to treat and prevent AMD. Methods We queried National Center for Biotechnology Information to identify genes associated with AMD, wet AMD, dry AMD, intermediate AMD, and geographic atrophy to date. We combined genes from various AMD subtypes to reflect distinct stages of disease. Enrichment analysis using the ToppGene platform predicted molecules that can influence AMD genes. Compounds without clinical indications or with deleterious effects were manually filtered. Results We identified several drug/chemical classes that can affect multiple genes involved in AMD. The drugs predicted from this analysis include antidiabetics, lipid-lowering agents, and antioxidants, which could theoretically be repurposed for AMD. Metformin was identified as the drug with the strongest association with wet AMD genes and is among the top candidates in all dry AMD subtypes. Curcumin, statins, and antioxidants are also among the top drugs correlating with AMD-risk genes. Conclusions We use a systematic computational process to discover potential therapeutic targets for AMD. Our systematic and unbiased approach can be used to guide targeted preclinical/clinical studies for AMD and other ocular diseases. Translational Relevance Advanced bioinformatics models identify novel chemicals and approved drug candidates that can be efficacious for different subtypes of AMD.

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Ophthalmic Nanosystems with Antioxidants for the Prevention and Treatment of Eye Diseases

Cited by 11 publications

References 66 publications

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

Resveratrol-Loaded Hydrogel Contact Lenses with Antioxidant and Antibiofilm Performance

Using Advanced Bioinformatics Tools to Identify Novel Therapeutic Candidates for Age-Related Macular Degeneration

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