Opioid receptors in human neuroblastoma SH-SY5Y cells: Evidence for distinct morphine (μ) and enkephalin (δ) binding sites

Kazmi, Syed M.I.; Mishra, Ram K.

doi:10.1016/0006-291x(86)91152-6

Cited by 40 publications

(15 citation statements)

References 11 publications

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“…This cell line was subcloned from the SK-N-SH line, and express mu and delta opioid receptors (Kazmi and Mishra, 1986; Yu and Sadee, 1988). Like its parent line, SH-SY5Y cells express delta opioid receptors to a lesser extent than mu (Kazmi and Mishra, 1987; Yu and Sadee, 1988).…”

Section: Introductionmentioning

confidence: 99%

The delta opioid receptor tool box

2016

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In recent years, the delta opioid receptor has attracted Increasing Interest as a target for the treatment of chronic pain and emotional disorders. Due to their therapeutic potential, numerous tools have been developed to study the delta opioid receptor from both a molecular and a functional perspective. This review summarizes the most commonly available tools, with an emphasis on their use and limitations. Here, we describe (1) the cell-based assays used to study the delta opioid receptor. (2) The features of several delta opioid receptor ligands, including peptide and non-peptide drugs. (3) The existing approaches to detect delta opioid receptors in fixed tissue, and debates that surround these techniques. (4) Behavioral assays used to study the in vivo effects of delta opioid receptor agonists; including locomotor stimulation and convulsions that are induced by some ligands, but not others. (5) The characterization of genetically modified mice used specifically to study the delta opioid receptor. Overall, this review aims to provide a guideline for the use of these tools with the final goal of increasing our understanding of delta opioid receptor physiology.

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Section: Introductionmentioning

confidence: 99%

The delta opioid receptor tool box

2016

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“…British Journal of Pharmacology (1996) 117,[333][334][335][336][337][338][339][340] in expressing both yi and 6 opioid receptors (Sonnenfeld et al, 1985;Kazmi & Mishra 1986). Both yu and 6 receptors inhibit N-type Ca channels (Seward et al, 1990; and adenylyl cyclase (Kazmi & Mishra, 1987;Yu & Sadee, 1988) in SH-SYSY cells.…”

Section: Sh-sy5y Cells Are a Human Neuroblastoma Cell Line Uniquementioning

confidence: 99%

δ‐ and μ‐opioid receptor mobilization of intracellular calcium in SH‐SY5Y human neuroblastoma cells

Connor

Henderson

1996

British J Pharmacology

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“…SH‐SY5Y cells naturally express several nAChR subunits (α3, α5, α7, β2, β4) (Peng et al . 1997) as well as µ‐ and δ‐opioid receptors (Kazmi and Mishra 1986). SH‐EP1‐hα7 cells are transfected with the α7 nAChR gene (Peng et al .…”

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confidence: 99%

Methadone increases intracellular calcium in SH‐SY5Y and SH‐EP1‐hα7 cells by activating neuronal nicotinic acetylcholine receptors

Pakkanen

Nousiainen

Yli‐Kauhaluoma³

et al. 2005

Journal of Neurochemistry

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(-)-Methadone acts as an agonist at opioid receptors. Both (+)-and (-)-enantiomers of methadone have been suggested to be potent non-competitive antagonists of a3b4 neuronal nicotinic acetylcholine receptors (nAChRs). In the present study, we have examined interactions of methadone with nAChRs by using receptor binding assays, patch-clamp recording and calcium fluorometry imaging with SH-SY5Y cells naturally expressing a7 and a3* nAChR subtypes and SH-EP1-ha7 cells heterologously expressing human a7 nAChRs. Methadone potently inhibited binding of [ 3 H]methyllycaconitine to a7 nAChRs and that of [ 3 H]epibatidine to a3* nAChRs. Methadone pretreatment induced up-regulation of epibatidine binding sites in SH-SY5Y cells. Using whole-cell patch-clamp recording, both isomers of methadone activated cation currents via mecamylamine-sensitive nAChRs in SH-SY5Y cells. Nicotine and both (+)-and (-)-methadone evoked increases in [Ca 2+ ] i in both fluo-3AM loaded cell lines, and these effects were blocked by mecamylamine and by the a7 selective antagonist methyllycaconitine, suggesting effects of methadone as a7-nAChR agonist. Sensitivity of sustained nicotine and methadone effects to blockade by CdCl 2 , ryanodine and xestospongin-c implicates voltage-operated Ca 2+ channels and intracellular Ca 2+ stores as downstream modulators of elevated [Ca 2+ ] i . Collectively, our results suggest that methadone engages in complex and potentially pharmacologically significant interactions with nAChRs.

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Opioid receptors in human neuroblastoma SH-SY5Y cells: Evidence for distinct morphine (μ) and enkephalin (δ) binding sites

Cited by 40 publications

References 11 publications

The delta opioid receptor tool box

The delta opioid receptor tool box

δ‐ and μ‐opioid receptor mobilization of intracellular calcium in SH‐SY5Y human neuroblastoma cells

Methadone increases intracellular calcium in SH‐SY5Y and SH‐EP1‐hα7 cells by activating neuronal nicotinic acetylcholine receptors

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