Opisthorchis viverrini: Analysis of the sperm-specific rhophilin associated tail protein 1-like

Rattanachan, Sitthichon; Grams, Rudi; Tesana, Smarn; Smooker, Peter M.; Grams, Suksiri Vichasri

doi:10.1016/j.actatropica.2014.08.002

Cited by 5 publications

(9 citation statements)

References 22 publications

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“…This is supported by the study of Zhang et al, with the inclusion that ROPN1L and ROPN1may be involved in sperm capacitation 82 . Another study also reported the presence of ROPN1L on the spermatozoa tail of Opisthorchis viverrini and that the sera of individuals suffering from opisthorchiasis showed reactivity to this protein 80 . The presence of ROPN1L on the spermatozoa tail suggests it may play a role in sperm motility.…”

Section: Resultsmentioning

confidence: 85%

“…Both ROPN1Land ROPN1binds AKAP3 via an N-terminal located domain present in other proteins 78 , 79 . Although the biochemical actions of ROPN1L are mainly unknown, it was suggested to regulate the activity of AKAP3, such as cAMP-dependent protein kinase 80 . Defects in sperm morphology were observed in the testes of ropn1l and ropn1 knockout mice, with a reduction in AKAP3 levels 81 .…”

Section: Resultsmentioning

confidence: 99%

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Using publicly available transcriptomic data to identify mechanistic and diagnostic biomarkers in azoospermia and overall male infertility

Omolaoye

Hachim

Plessis

2022

Sci Rep

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Azoospermia, which is the absence of spermatozoa in an ejaculate occurring due to defects in sperm production, or the obstruction of the reproductive tract, affects about 1% of all men and is prevalent in up to 10–15% of infertile males. Conventional semen analysis remains the gold standard for diagnosing and treating male infertility; however, advances in molecular biology and bioinformatics now highlight the insufficiency thereof. Hence, the need to widen the scope of investigating the aetiology of male infertility stands pertinent. The current study aimed to identify common differentially expressed genes (DEGs) that might serve as potential biomarkers for non-obstructive azoospermia (NOA) and overall male infertility. DEGs across different datasets of transcriptomic profiling of testis from human patients with different causes of infertility/ impaired spermatogenesis and/or azoospermia were explored using the gene expression omnibus (GEO) database. Following the search using the GEOquery, 30 datasets were available, with 5 meeting the inclusion criteria. The DEGs for datasets were identified using limma R packages through the GEO2R tool. The annotated genes of the probes in each dataset were intersected with DEGs from all other datasets. Enriched Ontology Clustering for the identified genes was performed using Metascape to explore the possible connection or interaction between the genes. Twenty-five DEGs were shared between most of the datasets, which might indicate their role in the pathogenesis of male infertility. Of the 25 DEGs, eight genes (THEG, SPATA20, ROPN1L, GSTF1, TSSK1B, CABS1, ADAD1, RIMBP3) are either involved in the overall spermatogenic processes or at specific phases of spermatogenesis. We hypothesize that alteration in the expression of these genes leads to impaired spermatogenesis and, ultimately, male infertility. Thus, these genes can be used as potential biomarkers for the early detection of NOA.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Using publicly available transcriptomic data to identify mechanistic and diagnostic biomarkers in azoospermia and overall male infertility

Omolaoye

Hachim

Plessis

2022

Sci Rep

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“…Interestingly, a sperm protein of O. viverrini , rhophilin associated tail protein 1-like (OvROPN1L) protein was recognized by sera of O. viverrini -infected humans and animals. OvROPN1L shows 47.2% sequence identity with the human homolog [ 8 ].…”

mentioning

confidence: 99%

“…In mammals, rhophilin-associated tail protein has been investigated how it is involved in the cAMP-dependent protein kinase pathway, and how to interact with A-kinase anchor protein, and how it affects sperm motility [ 10 – 12 ]. In trematodes, only OvROPN1L has been molecularly characterized [ 8 ]. OvROPN1L was already expressed in 2-week-old juveniles which could be an advantage if it can be applied for diagnosis [ 8 ].…”

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confidence: 99%

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Evaluation of Rhophilin Associated Tail Protein (ROPN1L) in the Human Liver Fluke Opisthorchis viverrini for Diagnostic Approach

et al. 2020

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Tegumental and excretory-secretory proteins are reported as diagnostic antigens for human opisthorchiasis. Rhophilin associated tail protein1-like (OvROPN1L) protein of Opisthorchis viverrini sperm tail showed potential as a diagnostic antigen. The OvROPN1L recombinant fragments were assayed for diagnostic antigenicity for human opisthorchiasis using indirect ELISA. The strongest antigenic region was a N-terminus peptide of M1 - P56. One synthetic peptide (P1, L3-Q13) of this region showed the highest antigenicity to opisthorchiasis. Sera from other parasitic infections including Strongyloides stercoralis, hookworm, Taenia spp, minute intestinal flukes, Paragonimus spp showed lower reactivity to P1. Peptide P1 is located in the disordered N-terminus of ROPN1L supporting its suitability as linear epitope. In the Platyhelminthes the N-terminal sequence of ROPN1L is diverging with taxonomic distance further suggesting that peptide P1 has potential as diagnostic tool in the genus Opisthorchis/Clonorchis. It should be further evaluated in combination with peptides derived from other O. viverrini antigens to increase its diagnostic power.

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Recent Advances in the Diagnosis and Detection of Opisthorchis viverrini Sensu Lato in Human and Intermediate Hosts for Use in Control and Elimination Programs

Saijuntha

Duenngai

Tangkawattana

et al. 2018

Advances in Parasitology

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Opisthorchis viverrini: Analysis of the sperm-specific rhophilin associated tail protein 1-like

Cited by 5 publications

References 22 publications

Using publicly available transcriptomic data to identify mechanistic and diagnostic biomarkers in azoospermia and overall male infertility

Using publicly available transcriptomic data to identify mechanistic and diagnostic biomarkers in azoospermia and overall male infertility

Evaluation of Rhophilin Associated Tail Protein (ROPN1L) in the Human Liver Fluke Opisthorchis viverrini for Diagnostic Approach

Recent Advances in the Diagnosis and Detection of Opisthorchis viverrini Sensu Lato in Human and Intermediate Hosts for Use in Control and Elimination Programs

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