In this study, the oil obtained from hawthorn (Crataegus monogyna), lemon balm (Melissa officinalis), opium poppy (Papaver somniferum), rosemary (Rosmarinus officinalis) known to have sedative and anesthetic efficacy in traditional medicine were determined biochemical and hematological changes in blood of rainbow throut (Oncorhynchus mykiss) in 96 h and a concentration of 25 mg/L. There were statistically significant increases in the levels of ALT (alanin aminotransferaz), ALP (alkalen fosfataz), AST (aspartat aminotransferaz), LDH (laktat dehidrogenaz), glucose, cortisol, total protein, urea and creatinine levels in the groups treated with hawthorn, lemon balm and opium poppy when compared with the control group (P<0.05) and total cholesterol, triglyceride, sodium, potassium and iron levels were found significant reductions (P<0.05). In hematological parameters; there were significant increases (P<0.05) in erythrocyte, hemoglobin and hematocrit levels of fish treated with hawthorn and opium poppy oil and decrease in leukocyte count (P<0.05). Biochemical and hematological results have shown that hawthorn, opium poppy and lemon balm oil can also be used for sedation and anesthesia when compared to blood parameters obtained from studies with 25-50 mg/L concentration of clove oil commonly used as a natural agent in fish anesthesia. It is believed that hawthorn, opium poppy and lemon balm oil applied in concentration of 25 mg/L for 96 h may be used as an alternative antioxidant supplement in the regulation of the physiological functions of fish and may provide positive contributions to the health of the consuming organisms.