In thermoelectric industry, synthetic gypsum and coal ash are generated as industrial by-products. The objective of this work was to evaluate the effect of different mixtures of synthetic gypsum and coal ash on N mineralization in an Alfisol. Nitrogen mineralization was evaluated by soil incubations under controlled conditions and potential mineralization using the nonlinear first order kinetics model Nm t = N 0 [1 -℮ (-kt) ], where Nm t is the mineral N accumulated at a specific time, N 0 is the potentially mineralizable N, k is the mineralization rate, and finally t is the incubation time. Treatments consisted of different mixtures of synthetic gypsum, coal ash and urea (M 1 : 50% synthetic gypsum, 50% coal ash, M 2 : 50% synthetic gypsum, 35% coal ash, 15% urea, M 3 : 45% synthetic gypsum; 40% coal ash, 15% urea, M 4 : 65% synthetic gypsum, 20% coal ash, 15% urea, M 5 : 55% synthetic gypsum, 30% coal ash, 15% urea). Each mixture was applied to the soil at 2 t ha ). Where the highest and lowest k were determined in M 5 (k = 0.44990) and M 3 (k = 0.04470), respectively. Mixtures of synthetic gypsum, coal ash and urea increased N mineralization over mixtures without urea. Respect N potential mineralization, in some cases N 0 and k values would respond more to the fit of the mathematical model employed than to the biological process.