2008
DOI: 10.1371/journal.pone.0004090
|View full text |Cite
|
Sign up to set email alerts
|

Opposing Effects of Sirtuins on Neuronal Survival: SIRT1-Mediated Neuroprotection Is Independent of Its Deacetylase Activity

Abstract: BackgroundGrowing evidence suggests that sirtuins, a family of seven distinct NAD-dependent enzymes, are involved in the regulation of neuronal survival. Indeed, SIRT1 has been reported to protect against neuronal death, while SIRT2 promotes neurodegeneration. The effect of SIRTs 3–7 on the regulation of neuronal survival, if any, has yet to be reported.Methodology and Principal FindingsWe examined the effect of expressing each of the seven SIRT proteins in healthy cerebellar granule neurons (CGNs) or in neuro… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

18
158
1
1

Year Published

2010
2010
2020
2020

Publication Types

Select...
6
1

Relationship

1
6

Authors

Journals

citations
Cited by 167 publications
(178 citation statements)
references
References 67 publications
18
158
1
1
Order By: Relevance
“…Cerebellar Granule Neuron Cell Culture, Transfection, and Viability Assays-CGNs were cultured from dissociated cerebella of 7-8-day-old Wistar rats as described previously (6,7,9). In short, processed CGNs were plated in basal Eagle's medium with Earle's salts (BME) supplemented with 10% FBS, 25 mM KCl, 2 mM glutamine (Invitrogen), and 100 g/ml gentamicin.…”
Section: Methodsmentioning
confidence: 99%
See 3 more Smart Citations
“…Cerebellar Granule Neuron Cell Culture, Transfection, and Viability Assays-CGNs were cultured from dissociated cerebella of 7-8-day-old Wistar rats as described previously (6,7,9). In short, processed CGNs were plated in basal Eagle's medium with Earle's salts (BME) supplemented with 10% FBS, 25 mM KCl, 2 mM glutamine (Invitrogen), and 100 g/ml gentamicin.…”
Section: Methodsmentioning
confidence: 99%
“…Cells were then maintained for 4 -5 days in 24-well dishes prior to transient transfection experiments. Transfection of CGNs was done as described previously (6,7,9). Briefly, 1 g/well of DNA was precipitated by the calcium-phosphate method for 30 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…It may be because the N terminus of SIRT1 could alter conformation of SUV39H1 by direct interaction (41). Indeed, SIRT1 can utilize a noncatalytic mechanism to protect neuron survival (42). Further studies are needed to elucidate whether the binding of SIRT1 to bZIP domain of c-Fos and c-Jun participates in AP-1 transcriptional regulation in a deacetylase-independent manner.…”
Section: Sirt1 Is a Nadmentioning
confidence: 99%