1988
DOI: 10.1128/mcb.8.4.1657
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Opposite replication polarities of transcribed and nontranscribed histone H5 genes.

Abstract: We used an in vitro nuclear runoff replication assay to analyze the direction of replication of the active and inactive histone H5 genes in avian cells. In embryonic erythrocytes the transcribed histone H5 gene displayed sensitivity to endogenous nuclease cleavage. In contrast, this gene was insensitive to endogenous nuclease digestion under the same conditions in nuclei of the lymphoblastoid cell line MSB-1, and histone H5 gene transcripts were not detectable by dot-blot analysis of MSB-1 cell RNA. When nucle… Show more

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Cited by 36 publications
(32 citation statements)
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“…However, studies with Drosophila melanogaster and amphibian egg reference 34). These suggestions are compatible with the recent observations that in in vitro replication assays, the transcriptionally active histone H5 genes in chicken embryonic erythrocytes are replicated in the transcriptional direction from an origin located 5' to the gene, whereas the inactive H5 genes are replicated in the opposite direction from a downstream origin (37). Furthermore, studies on the temporal order of replication of the immunoglobulin heavychain cluster (IgCH) in mouse cell lines is also consistent with replication proceeding from a presumptive origin downstream of the gene cluster when the locus is inactive (5).…”
supporting
confidence: 80%
“…However, studies with Drosophila melanogaster and amphibian egg reference 34). These suggestions are compatible with the recent observations that in in vitro replication assays, the transcriptionally active histone H5 genes in chicken embryonic erythrocytes are replicated in the transcriptional direction from an origin located 5' to the gene, whereas the inactive H5 genes are replicated in the opposite direction from a downstream origin (37). Furthermore, studies on the temporal order of replication of the immunoglobulin heavychain cluster (IgCH) in mouse cell lines is also consistent with replication proceeding from a presumptive origin downstream of the gene cluster when the locus is inactive (5).…”
supporting
confidence: 80%
“…These findings suggest that in vivo, transcription of the c-myc gene may play a role in the initiation of DNA replication from a region upstream of the gene. A correlation between initiation of DNA replication and gene expression has also been reported for the human ␤-globin (23) and ppv1 (7) genes, the Physarum profilin P gene (4), and the avian histone H5 gene (51). From the in vitro study described here and these in vivo observations, transcription may play an important role in the activation of replication origins located in the promoter regions by generating negative supercoiling within these regions.…”
Section: Discussionmentioning
confidence: 63%
“…For example, arrest sites may act by regulating recombination or by promoting differential chromatin assembly of sister chromatids at key developmental loci during replication (Seidman et al 1979;Shinahara and Stillman 1999). In support of this possibility, differences in the direction of replication have been observed between expressed and unexpressed genes in human cell lines (Trempe et al 1988;Leffak and James 1989). Whether replication arrest sites are involved in regulation at these genetic loci remains to be explored.…”
Section: Discussionmentioning
confidence: 74%