Opracowanie metodyki oznaczania polichlorowanych bifenyli w próbkach gleb

Wojtowicz, Katarzyna; Badawczy, Instytut Nafty i Gazu – Państwowy Instytut; Jakubowicz, P.

doi:10.18668/ng.2019.07.06

Cited by 2 publications

(3 citation statements)

References 10 publications

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“…Ten grams of dried soil were placed in an Erlenmeyer flask and extracted using a mixture (80 mL) of n-hexane and dichloromethane (POCH S.A., Gliwice, Poland) at 1:1 ratio [90]. The PCBs were extracted on a Conbest DOS-20L shaker for 3 h, at a constant temperature of 30 • C and shaking rate of 150 rpm [91]. After 3 h, 1 g of powdered copper (Merck, Darmstadt, Germany) was added to the extract and the mixture was extracted for another hour [19].…”

Section: Pcb Extraction and Quantificationmentioning

confidence: 99%

Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

et al. 2020

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Biodegradation is one of the most effective and profitable methods for the elimination of toxic polychlorinated biphenyls (PCBs) and total petroleum hydrocarbons (TPH) from the environment. In this study, aerobic degradation of the mentioned pollutants by bacterial strains Mycolicibacterium frederiksbergense IN53, Rhodococcus erythropolis IN129, and Rhodococcus sp. IN306 and mixed culture M1 developed based on those strains at 1:1:1 ratio was analyzed. The effectiveness of individual strains and of the mixed culture was assessed based on carried out respirometric tests and chromatographic analyses. The Rhodococcus sp. IN306 turned out most effective in terms of 18 PCB congeners biodegradation (54.4%). The biodegradation index was decreasing with an increasing number of chlorine atoms in a molecule. Instead, the Mycolicobacterium frederiksbergense IN53 was the best TPH degrader (37.2%). In a sterile soil, contaminated with PCBs and TPH, the highest biodegradation effectiveness was obtained using inoculation with mixed culture M1, which allowed to reduce both the PCBs (51.8%) and TPH (34.6%) content. The PCBs and TPH biodegradation capacity of the defined mixed culture M1 was verified ex-situ with prism method in a non-sterile soil polluted with aged petroleum hydrocarbons (TPH) and spent transformer oil (PCBs). After inoculation with mixed culture M1, the PCBs were reduced during 6 months by 84.5% and TPH by 70.8% as well as soil toxicity was decreased.

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Section: Pcb Extraction and Quantificationmentioning

confidence: 99%

Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

et al. 2020

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“…The chromatographic analysis was carried out on a Perkin Elmer Clarus 500 chromatograph, equipped with a Restek capillary column RTX-XLB (30 m × 0.32 µm), using the following temperature parameters: injector temperature = 280°C, ECD detector temperature = 320°C, oven temperature programme: 120°C -isothermal course for 2 min., 120-280°C -temperature increase at a rate of 15°C/min., 280°C-isothermal course for 5 min., 280-320°C -temperature increase at a rate of 28°C/min., 320°C -isothermal course for 10 min. (Wojtowicz and Jakubowicz, 2019). A certified Sigma-Aldrich PCB Congeners in Soil SQC068-50g standard of sterile soil was used for qualitative and quantitative determination of individual PCB congeners.…”

Section: Chromatographic Analysis Of Pcbsmentioning

confidence: 99%

“…2. Chromatographic analysis enables qualitative and quantitative determination of individual PCB congeners during the process of soil cleaning (Wojtowicz and Jakubowicz, 2019).…”

Section: Toxicological Assessment Of the Soilmentioning

confidence: 99%

Study on bioremediation of soil contaminated with polychlorinated biphenyls (PCBs)

Wojtowicz

Steliga

2020

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The article presents issues related to biodegradation of polychlorinated biphenyls in soil by inoculation with a biopreparation IN-3 developed on the basis of microorganisms capable of metabolizing PCB. Biodegradation tests were carried out using the ex-situ technological pile method on a specially constructed test stand. As part of the research, physical and chemical analyzes of the initial soil were performed, with particular regard to the content of nutrients (nitrogen and phosphorus), PCB chromatographic analyzes and toxicological analyzes. Before inoculating soil with biopreparation IN-3, the parameters of the process were established (temperature = 17-25°C, humidity = 20-25%, pH = 7.5-7.8, content of nutrients: C:N:P = 100:10:1), which were monitored during bioremediation. During soil treatment, the content of identified PCB congeners was monitored. The assessment of biodegradation efficiency of polychlorinated biphenyls in soil was complemented by toxicological monitoring, which was carried out using live organisms representing all trophic levels: producers (Phytotoxkit TM test), consumers (Ostracodtoxkit F TM test) and reducers agents (Microtox ® SPT test). Also, the presence of mutagenic factors were tested with the Ames mutagenicity test. As a result of the six-month biodegradation process of soil contaminated with aged transformer oil, upon inoculation with biopreparation IN-3, a reduction in the content of polychlorinated biphenyls from 13 153.9 µg/kg d.m. down to 1650.4 µg/kg d.m. (87.5%) was archieved. The obtained chromatographic analysis results showed that the efficiency of the PCB biodegradation depended on the number of chlorine atoms in the biphenyl ring (with the increase in the number of chlorine atoms in the PCB molecule the degree of biodegradation decreased) and the structure of the molecule (PCB congeners having 2 or more chlorine atoms in the biphenyl ring ortho positions were more difficult to biodegrade than non-ortho or mono-ortho, while polychlorinated biphenyls, in which chlorine atoms occur on only one phenyl ring, were more easily biodegradable). The toxicological analysis showed a reduction in the toxicity of the soil tested from toxicity unit TU = 26.7 to TU = 6.1. In addition, toxkit tests (Phytotoxkit TM and Ostracodtoxkit F TM ) showed a decrease in inhibition of test organism growth from 69.3 to 14.8% (Ostracodtoxkit F TM test) and from 64.7-78.0 to 11.9-17.5% (Phytotoxkit test). Decreasing the mutagenicity index from 8.3 to 1.5 in the Ames test confirmed the effectiveness of the purification process. Based on the results of the experiment, conclusions were made regarding issues related to biodegradation of polychlorinated biphenyls in soil.

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Opracowanie metodyki oznaczania polichlorowanych bifenyli w próbkach gleb

Cited by 2 publications

References 10 publications

Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture

Study on bioremediation of soil contaminated with polychlorinated biphenyls (PCBs)

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