Opsonic activity of plasma fibronectin for Staphylococcus aureus by human alveolar macrophages: Inefficacy of trypsin-sensitive staphylococcal fibronectin receptor.

Oishi, Kazunori; Yamamoto, Masashi; Yoshida, Toshiaki; Ide, Masatoshi; Matsumoto, Keizō

doi:10.1620/tjem.149.95

Cited by 3 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the absence of mycoplasmal killing in C3H/HeN mice agrees with findings of previous in vitro experiments that demonstrated a lack of activity against M. pulmonis by mouse macrophages in the absence of specific serum antibodies (5,9,16,18), killing of M. pulmonis by C57BL/6N mice presents a paradox. Pulmonary clearance of bacteria was initially ascribed to the activity of alveolar macrophages (14), but experiments with Staphylococcus aureus (21,29,30) and Klebsiella pneumoniae (32) have recently shown that bactericidal activity in the lungs is not solely dependent on alveolar macrophages alone. Thus, although alveolar macrophages may play a role in clearance of M. pulmonis from the lungs, other factors are likely also involved.…”

Section: Mrmmentioning

confidence: 99%

Pulmonary clearance of Mycoplasma pulmonis in C57BL/6N and C3H/HeN mice

et al. 1987

View full text Add to dashboard Cite

In C57BL/6N and C3H/HeN mice known to be free of all murine pathogens and matched for age, sex, and environmental factors, pulmonary clearance was measured over a 72-h time period after exposure to infectious aerosols of 35S-labeled Mycoplasma pulmonis. Reduced clearance of M. pulmonis in C3H/HeN mice relative to C57BL/6N mice was primarily due to impaired mycoplasmacidal activity in the lungs of the C3H/HeN mice. The C3H/HeN mice also had a slightly slower rate of mechanical transport of radiolabel from the lungs in the first 4 h after infection relative to the C57BL/6N mice but not at any later times. By 72 h after infection (relative to 0 h, C3H/HeN mice had an over 4,000% (1.75 X 10(7) versus 4.30 X 10(5] increase in neutrophils and an over 18,000% (more than 2 orders of magnitude) increase in numbers of M. pulmonis recovered from mechanically disaggregated lungs. In contrast, C57BL/6N mice reduced the number of M. pulmonis present by over 83% (nearly 2 orders of magnitude) before any increase in inflammatory cells, which was only a slight increase in lymphocytes and macrophages at 24 h after infection. These results directly link decreased mycoplasmal pulmonary clearance in C3H/HeN mice with the increased susceptibility to, and severity of, murine respiratory mycoplasmosis observed in this strain. The resistance of C57BL/6N mice appears to be related to nonspecific host defense mechanisms responsible for limiting the extent of infection.

show abstract