1989
DOI: 10.1111/j.1365-3024.1989.tb00665.x
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Opsonization of yeast by the plasma of Biomphalaria glabrata (Gastropoda): a strain‐specific, time‐dependent process

Abstract: Phagocytosis of yeast by haemocytes of Biomphalaria glabrata, intermediate host of Schistosoma mansoni, is influenced strongly by host plasma components, although it can occur without involvement of such factors. Plasma from two strains of B. glabrata which are resistant to S. mansoni differs in its opsonic properties from the plasma of a susceptible strain. This may reflect the principles which determine compatibility or incompatibility in this host-parasite system. Opsonization is a time-dependent process: s… Show more

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Cited by 49 publications
(16 citation statements)
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“…The fact that the SOD activity recovered later than that of the respiratory burst suggests that the shrimp received noradrenaline causes immunomodulation to scavenge superoxide anion to other reactive oxygen intermediates (ROIs) including H 2 O 2 . Phagocytosis is an important cellular defence mechanism, whereas clearance efficiency is an important humoral defence mechanism in molluscs and crustaceans [34,35]. A significant reduction of phagocytic activity and clearance efficiency against V. alginolyticus was observed in the white shrimp L. vannamei following exposure to 1 mg l ÿ1 Cu 2C [20], to 11.21 mg l ÿ1 ammonia-N [4], and when transferred to 5 and 15& from 25& seawater [6].…”
Section: Discussionmentioning
confidence: 99%
“…The fact that the SOD activity recovered later than that of the respiratory burst suggests that the shrimp received noradrenaline causes immunomodulation to scavenge superoxide anion to other reactive oxygen intermediates (ROIs) including H 2 O 2 . Phagocytosis is an important cellular defence mechanism, whereas clearance efficiency is an important humoral defence mechanism in molluscs and crustaceans [34,35]. A significant reduction of phagocytic activity and clearance efficiency against V. alginolyticus was observed in the white shrimp L. vannamei following exposure to 1 mg l ÿ1 Cu 2C [20], to 11.21 mg l ÿ1 ammonia-N [4], and when transferred to 5 and 15& from 25& seawater [6].…”
Section: Discussionmentioning
confidence: 99%
“…In the case of B. glab- rata and S. mansoni, it has been reported that two susceptible strains had fewer circulating haemocytes than a resistant strain (Coustau & Yoshino, 1994a). However, resistance may also be related to qualitative changes in soluble plasma factors (Fryer & Bayne, 1989) or haemocyte components (Yoshino & Granath, 1985;Coustau & Yoshino, 1994b). The existence of a cost of resistance has a practical impact on the maintenance of resistant strains in the laboratory.…”
Section: Discussionmentioning
confidence: 99%
“…Particularmente, en moluscos bivalvos se ha estudiado la fagocitosis en los hemocitos de la almeja Tapes philippinarum frente al género Perkinsus (Villalba et al 2004), en los gastrópodos Biomphalaria glabrata (Fryer & Bayne, 1996;Matricon-Gondran & Letocart, 1999) y en Lymnaea stagnalis (Amen et al, 1992;Zelck et al, 2005), estos dos últimos se utilizan como modelos para estudiar la relación huésped-parásito. Los hemocitos de A. purpuratus tienen una capacidad fagocítica basal de 13,3% (González & Arenas, 2002), lo que se repite en otras especies de invertebrados con rangos que varían entre 11 y 28% (Fryer & Bayne, 1989;Kelly et al, 1993;Ballarini et al, 1994;Blanco et al, 1995). En este trabajo se ha demostrado que no existe diferencia significativa entre la capacidad fagocítica de los hemocitos circulantes de ostiones con RM respecto a los controles, lo que indica que estas células no se ven afectadas en esta función respecto a la presencia o ausencia del cuadro descrito.…”
Section: Discussionunclassified
“…El porcentaje de fagocitosis aumentó significativamente en ambos grupos cuando las levaduras se incubaron con suero de ejemplares SRM; al contrario, disminuyó significativamente con el suero de ostiones con RM, lo que indicaría que el suero de estos ejemplares carecería o tendría bajas concentraciones de molé-culas con capacidad opsonizadora que facilitan la fagocitosis. Resultados similares han sido reportados en el caracol Biomphalaria glabrata parasitado con Schistosoma mansoni, frente a levaduras opsonizadas y no opsonizadas (Fryer & Bayne, 1989). Estas moléculas podrían corresponder a proteínas que se asocian a lipopolisacáridos y beta-glucanos como las descritas para los pectínidos Chlamys farreri y Argopecten irradians (Su et al, 2004;Ni et al, 2007).…”
Section: Discussionunclassified