OPSONIZING AND 32 P‐RELEASING ACTIVITIES OF RAT INTESTINAL STRANGULATION FLUID ON 32 P‐LABELLED E. COLI

Acta Pathologica Microbiologica Scandinavica Section B Microbio

1973

Self Cite

Exudate collected from a strangulated loop of intestine in conventional and germfree rats has the property of inhibiting the opsonic and bactericidal activity of normal rat serum on Escherichiu coli. Using release of radioactivity from "P-labelled E. coli as an indicator of bactericidal activity, the inhibitory property of strangulation fluid on serum bactericidal activity has been subjected to further studies. The inhibitory factor (FIB) resisted heat treatment for 30 min up to 100" C, and did not disappear following dialysis. Chromato-

Section: Strangulation Fluidmentioning

confidence: 99%

STUDIES ON A FACTOR IN INTESTINAL STRANGULATION FLUID FROM GERMFREE AND CONVENTIONAL RATS INHIBITING SERUM BACTERICIDAL ACTIVITY ON ESCHERICHIA COLI

Acta Pathologica Microbiologica Scandinavica Section B Microbio

1973

Self Cite

“…The findings indicate that tlie filtrate contains substances which stimulate the bactericidal activity of serum. It may be recalled that "early" strangulation fluid filtrate also seemed to contain factors which were capable of enhancing the bactericidal capacity of serum, yet being unable to initiate the reaction by itself (9). I n a previous report (8) it was demonstrated that "late" strangulation fluid filtrate contained Substances which partly inactivated the bactericidal capacity of serum.…”

Section: Discussionmentioning

confidence: 99%

“…Fluid portions were pooled from 64 rats of both sexes. Sterile filtrates wrre prepared according to methods previously described ( 9 ) .…”

Section: Strangulation Fluidmentioning

confidence: 99%

INFLUENCE OF INTESTINAL STRANGULATION FLUID FROM RATS ON THE SERUM SENSITIVITY OF E. COLI

Acta Pathologica Microbiologica Scandinavica Section B Microbio

1970

Self Cite

I'lir results prrsented indicate that substances with different activities on E. coli are present in rxudatr produced in rats during the later stages of intestinal strangulation obstruction. The sterile filtered exudate contains substances which inhibit the bactericidal activity of normal scrim on the bacteria. These substances do not appear to be adsorbed to the bacteria. Other substances in the filtrate stimulate the deleterious effect of serum on E. coli. 'l'hrsc substanrcs arr probably ad5orbcd to the bacteria.

mentioning

confidence: 99%

“…Tlir effects of strangulation fluid filtrates from conventional rats on host defence reactions have been studird previously (20,21). Sterile filtrates of such fluid show less opsonic and bactericidal activities on E. coli than does normal rat serum (21). The filtrate of fluid obtained later than 48 hours after operation also appears to interfere with the opsonic and bactericidal capacities of normal rat serum (20).…”

mentioning

confidence: 99%

Host Defence Reactions During Intestinal Strangulation Obstruction in Germfree Rats

Acta Pathologica Microbiologica Scandinavica Section B Microbio

1970

Self Cite

Exudate was collected from germfree rats subjected to experimrntal intestinal strangulation obstruction. The exudate, called strangulation fluid, was non-lethal when injected into the peritoneal cavity of mice. The fluid was found to enhance the virulence of E. coli towards mice. Strangulation fluid from germfree rats appeared to contain less opsonic and bactericidal properties than serum from germfree rats towards E. coli. The fluid appeared to inactivate the opsonic and bactericidal activity of conventional rat scrum to a substantial degree.