Opsonophagocytic Activity and Other Serological Indications of<i>Bordetella pertussis</i>Infection in Military Recruits in Norway

Aase, Audun; Herstad, Tove Karin; Merino, Samuel; Brandsdal, Kari Torkildsen; Berdal, B. P.; Aleksandersen, Erja M.; Aaberge, Ingeborg S.

doi:10.1128/cvi.00081-07

Cited by 19 publications

(17 citation statements)

References 45 publications

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“…This is one of a few studies that document increased vaccine- induced OPA against pertussis in human sera after revaccination with an acellular pertussis vaccine (19,36,40). In an earlier study, we found increased OPA in sera from military recruits who also had corresponding high levels of antibodies against pertussis (1). Those antibodies likely were reminiscent of previous infections rather than induced by vaccination, and the specificities of these antibodies probably were directed against a whole range of B. pertussis epitopes.…”

Section: Discussionmentioning

confidence: 49%

“…OPA was measured as respiratory burst, as previously described (1). Briefly, live B. pertussis cells were used as target cells, and dihydrorhodamine 123 (Molecular Probes, Invitrogen)-primed polymorphonuclear neutrophils (PMNs) were used as effector cells.…”

Section: Bacterial Strainmentioning

confidence: 99%

“…However, the antibody specificity and antibody effector mechanisms for protection remain to be established. Phagocytosis may be an important mechanism to clear the bacteria, and we have shown previously that there is a high correlation between opsonophagocytic activity (OPA) and IgG antibodies against FHA and PT in sera from military recruits (1). Conflicting results have been found regarding the OPA and immunity to pertussis, and only a few studies have investigated the OPA in sera after immunization with acellular pertussis vaccine (19,36,40).…”

mentioning

confidence: 99%

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Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Aase¹,

Herstad²,

Merino³

et al. 2011

Clin. Vaccine Immunol.

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Waning vaccine-induced immunity against Bordetella pertussis is observed among adolescents and adults. A high incidence of pertussis has been reported in this population, which serves as a reservoir for B. pertussis. A fifth dose of reduced antigen of diphtheria-tetanus-acellular-pertussis and inactivated polio vaccine was given as a booster dose to healthy teenagers. The antibody activity against B. pertussis antigens was measured prior to and 4 to 8 weeks after the booster by different assays: enzyme-linked immunosorbent assays (ELISAs) of IgG and IgA against pertussis toxin (PT) and filamentous hemagglutinin (FHA), IgG against pertactin (PRN), opsonophagocytic activity (OPA), and IgG binding to live B. pertussis. There was a significant increase in the IgG activity against PT, FHA, and PRN following the booster immunization (P < 0.001). The prebooster sera showed a geometric mean OPA titer of 65.1 and IgG binding to live bacteria at a geometric mean concentration of 164.9 arbitrary units (AU)/ml. Following the fifth dose, the OPA increased to a titer of 360.4, and the IgG concentration against live bacteria increased to 833.4 AU/ml (P < 0.001 for both). The correlation analyses between the different assays suggest that antibodies against FHA and PRN contribute the most to the OPA and IgG binding.

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Section: Discussionmentioning

confidence: 49%

Section: Bacterial Strainmentioning

confidence: 99%

mentioning

confidence: 99%

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Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Aase¹,

Herstad²,

Merino³

et al. 2011

Clin. Vaccine Immunol.

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“…Despite the implementation of national programs for immunization against Bordetella pertussis (B. pertussis) and their acceptable vaccination coverage in many countries, this infection represents an important contribution to disease in different age subgroups, particularly in developing countries [1,2]. According to the reports of centers for disease control and prevention [3], B. pertussis remains the most commonly reported vaccine-preventable disease, especially in children.…”

Section: Introductionmentioning

confidence: 99%

Immunization status of Iranian military recruits against Bordetella pertussis infection (whooping cough)

Izadi

Afsharpaiman

Jafari

et al. 2011

J Infect Dev Ctries

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Introduction: Military recruits are susceptible to respiratory pathogens because of increased antibiotic resistance and the lack of an effective vaccine. The goal of the current study was to determine the immunological status of the Bordetella pertussis among conscripts in Iranian military garrisons. Methodology: The study population consisted of 424 conscripts aged 18 to 21 years who enrolled for military service. They were selected using cluster stratified sampling from all military garrisons in Tehra, Iran. To determine the seroprevalence of infection, blood specimens from all recruits were collected and stored at -20°C until assayed. All serum samples were screened for immunoglobulin G (IgG) antibodies against Bordetella pertussis toxin (PT) and by using enzyme-linked immunosorbent assay (ELISA). Results: The overall prevalence of B. pertussis seropositivity in military recruits was 60.6. Only 55.0% of the recruits had low awareness about the record of vaccination against B. pertussis during childhood. Among 424 studied individuals, 48 recruits (11.3%) had a positive history of whooping cough; prevalence of seropositivity in these recruits was 70.0%. Among these subjects, 61.7% were referred to a physician for treatment and only 39.6% of them were administered anti-pertussis therapy. Conclusions: Our study showed that military conscripts in Tehran garrisons were not serologically immune to pertussis and also confirmed the low awareness about vaccination and medical history related to pertussis infection in this high-risk subgroup of the Iranian population. Routine acellular booster vaccination, particularly before 18 years of age, is recommended.

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“…Opsonophagocytosis assays were developed for a range of bacteria (24)(25)(26)(27) and have been widely used to assess immune responses to pneumococcal vac- antibody-mediated C3b/iC3b deposition levels in mother and cord samples separately against GBS serotypes Ia (mother, n ϭ 497; cord, n ϭ 478), Ib (mother, n ϭ 535; cord, n ϭ 521), II (mother, n ϭ 505; cord, n ϭ 499), III (mother, n ϭ 500; cord, n ϭ 483), and V (mother, n ϭ 518; cord, n ϭ 509). FI-C=, fluorescence index minus complement only.…”

Section: Discussionmentioning

confidence: 99%

Antibody-Mediated Complement C3b/iC3b Binding to Group B Streptococcus in Paired Mother and Baby Serum Samples in a Refugee Population on the Thailand-Myanmar Border

Herbert¹,

Thomas²,

Brookes³

et al. 2015

Clin. Vaccine Immunol.

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f Streptococcus agalactiae (group B streptococcus [GBS]) is the leading cause of neonatal sepsis and meningitis. In this study, we determined antibody-mediated deposition of complement C3b/iC3b onto the bacterial cell surface of GBS serotypes Ia, Ib, II, III, and V. This was determined for 520 mother and umbilical cord serum sample pairs obtained at the time of birth from a population on the Thailand-Myanmar border. Antibody-mediated deposition of complement C3b/iC3b was detected to at least one serotype in 91% of mothers, despite a known carriage rate in this population of only 12%. Antibody-mediated C3b/iC3b deposition corresponded to known carriage rates, with the highest levels of complement deposition observed onto the most prevalent serotype (serotype II) followed by serotypes Ia, III, V, and Ib. Finally, neonates born to mothers carrying serotype II GBS at the time of birth showed higher antibody-mediated C3b/iC3b deposition against serotype II GBS than neonates born to mothers with no serotype II carriage. Assessment of antibody-mediated C3b/iC3b deposition against GBS may provide insights into the seroepidemiology of anti-GBS antibodies in mothers and infants in different populations.

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Opsonophagocytic Activity and Other Serological Indications ofBordetella pertussisInfection in Military Recruits in Norway

Abstract: Bordetella pertussis is the causative agent of pertussis (whooping cough)

Cited by 19 publications

References 45 publications

Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Immunization of Teenagers with a Fifth Dose of Reduced DTaP-IPV Induces High Levels of Pertussis Antibodies with a Significant Increase in Opsonophagocytic Activity

Immunization status of Iranian military recruits against Bordetella pertussis infection (whooping cough)

Antibody-Mediated Complement C3b/iC3b Binding to Group B Streptococcus in Paired Mother and Baby Serum Samples in a Refugee Population on the Thailand-Myanmar Border

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