Optic nerve transection and tectal removal affect phagocytic activity of the pigment epithelium in goldfish

Li, Winnie W. Y.; Hui, Betta S.W.; Tsang, D.; Yew, David T.

doi:10.1016/s0065-1281(87)80005-3

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…In addition, Müller cells have been reported to phagocytize egg-lecithin-' coated silicone particles following intraocular injection (Nishizona et al, 1993). Similarly, Müller cells in explant cultures of goldfish retina also accumulate latex beads, although no uptake was observed in vivo (Li et al, 1987;Wagner and Raymond, 1991). Phagocytosis of exogenous particles, cell debris, and hemorrhagic products may be an important scavenging func- Figure. 6.5.…”

Section: Phagocytosismentioning

confidence: 99%

The Retinal Müller Cell

2002

Perspectives in Vision Research

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Section: Phagocytosismentioning

confidence: 99%

The Retinal Müller Cell

2002

Perspectives in Vision Research

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“…It has also been documented that the cones in the ONL and the related cone bipolars in the INL were the hardest hit (Yew & Li, 1986). As a result, the intact cells in pigment epithelium in the retina displayed a decrease in phagocytic activity in goldfish and rat (Li, Hui, Tsang, & Yew, 1987;Yew et al, 1985). These cellular changes in the retina have been evident not only in the lower vertebrates but also in the higher vertebrates as well, for example in adult rats and rabbits (Steele, van Hof, van der Steen, & Collewijn, 1987;Tsang, Yew, & Lam, 1985).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Retinal Degeneration in the Normal Goldfish and the Megalophthalmic Black Moor Goldfish after Optic Nerve Transection and Lens Extraction

Xiao

Wai

Wang

et al. 2009

International Journal of Neuroscience

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A comparison in retinal degeneration was studied in the normal goldfish and the megalophthalmic goldfish after optic nerve transection or lens extraction by the TUNEL (Terminal transferase-mediated dUTP nick end labeling) technique. A significant number of TUNEL positive cells appeared in both cases 7 days after injury, with a more prominent result in the megalophthalmic eye. Lens extraction, had less apoptotic cell death in the experimental retinas.

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Müller glial cells of the goldfish retina are phagocytic in vitro but not in vivo

Wagner

Raymond

1991

Experimental Eye Research

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The role of Müller glial cells in the process of degeneration and regeneration of the goldfish retina is poorly understood. One potential role is phagocytosis of neuronal debris in degenerating retinas. We investigated the phagocytic capacity of Müller glial cells of the goldfish retina both in vitro and in vivo. Müller glial cells from primary or first passage cultures were incubated with latex beads to assess their phagocytic ability, and acridine orange staining was used to identify phagolysosomes in living Müller glial cells. These experiments showed that Müller glial cells are phagocytic in culture. Cell identity was verified with an antibody raised against glial fibrillary acidic protein (GFAP). For the in vivo experiments fluorescent latex beads alone or in combination with the metabolic poison ouabain were injected into the posterior chamber. At various intervals (4 days to 8 weeks) after injection the retinas were prepared for immunocytochemistry. Polyclonal anti-GFAP and NN-1, a monoclonal antibody which recognizes macrophages and microglia within the goldfish retina, were used to identify the phagocytic cells. When the beads were injected into the eye, they were phagocytosed by macrophages/microglia cells but not by Müller cells.

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Optic nerve transection and tectal removal affect phagocytic activity of the pigment epithelium in goldfish

Cited by 4 publications

References 7 publications

The Retinal Müller Cell

The Retinal Müller Cell

Comparison of Retinal Degeneration in the Normal Goldfish and the Megalophthalmic Black Moor Goldfish after Optic Nerve Transection and Lens Extraction

Müller glial cells of the goldfish retina are phagocytic in vitro but not in vivo

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