Rong Duan and XiTeng Liu. The Experimental Study on Optical Transfer Function under Coherent Light Based on MATLAB Simulation. Biomed J Sci & Tech Res 45(5)-2022. BJSTR. MS.ID.007257. The Optical Transfer Function (OTF) can transfer spectra of different frequencies.In the process of transmission, the frequency will not change, but the amplitude will decrease, and the phase will shift. Therefore, the OTF is widely used in the evaluation of system imaging quality. In this paper, the intensity distribution, the coherent transfer function, the optical transfer function and the cut-off frequency of the image are obtained by using the back of the "5-yuan'he'coin" as the object of MATLAB simulation. Finally, the experimental results are consistent with the theoretical analysis, which proves the accuracy of the MATLAB simulation measurement method. At the same time, the relevant theoretical analysis also provides an important theoretical basis for optimizing the system parameters and improving the correlation calculation of OTF. The experimental study of OTF has great potential applications in military and aerospace fields.