The optical properties of bovine ocular tissues have been determined at laser wavelengths in the near-infrared (NIR) region. The inverse adding doubling (IAD), Kubelka–Munk (KM), and inverse Monte Carlo (IMC) methods were applied to the measured values of the total diffuse transmission, total diffuse reflection, and collimated transmission to determine the optical absorption and scattering coefficients of the bovine cornea, lens and retina from 750 to 1,000 nm using a CW Ti:sapphire laser. The optical properties obtained from these three methods have been compared and are discussed.