This paper deals with the investigation of annealing e®ects on the structural, morphological and optical properties of V 2 O 5 nanorods (NRs) grown on the glass substrates by using chemical spray pyrolysis technique. The as-prepared samples were annealed at 500 for 40, 60 and 120 min in a quartz tube furnace. The high resolution X-ray di®raction (XRD) analysis revealed V 2 O 5 NRs with preferred orientation along (001) plane. The crystallite size of the V 2 O 5 NRs was increased by increasing the annealing duration. The morphological observations using¯eld emission scanning electron microscope (FESEM) displayed NRs structures whose diameter and length were found to increase with increase of the annealing duration. The transmission electron microscopy (TEM) analysis con¯rmed the orthorhombic structures of the NRs. The AFM measurements indicated an increase of the average surface roughness by increasing the annealing time. The Raman spectroscopy revealed V-O-V phonon mode in the NRs annealed for 120 min. The optical bandgap was found in the range 2.6-2.58 eV and observed to decrease with various duration annealed.