Optical and Magnetic Resonance Imaging of Cell Death and Platelet Activation Using Annexin A5-Functionalized Quantum Dots

Prinzen, Lenneke; Rj, Miserus; Dirksen, Anouk; Tm, Hackeng; Deckers, Niko; Nj, Bitsch; Megens, Remco T. A.; Douma, Kim; Heemskerk, Johan W. M.; Kooi, M. Eline; Pm, Frederik; Dw, Slaaf; Zandvoort, van; Cp, Reutelingsperger

doi:10.1021/nl062226r

Cited by 152 publications

(99 citation statements)

References 39 publications

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“…The ionic T 1 relaxivity of cNGRpQDs, i.e., per Gd ion, was 7.1 F 0.4 mmol/L À1 s À1 at 7 T and 20jC, which lies in the expected range for macromolecular contrast agents and is in correspondence with previously reported values for Annexin A5 conjugated pQDs (37).…”

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confidence: 90%

Quantitative Molecular Magnetic Resonance Imaging of Tumor Angiogenesis Using cNGR-Labeled Paramagnetic Quantum Dots

et al. 2008

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The objective of this study was to develop and apply cyclic Asn-Gly-Arg (cNGR)-labeled paramagnetic quantum dots (cNGR-pQDs) for the noninvasive assessment of tumor angiogenic activity using quantitative in vivo molecular magnetic resonance imaging (MRI). cNGR was previously shown to colocalize with CD13, an aminopeptidase that is highly overexpressed on angiogenic tumor endothelium. Because angiogenesis is important for tumor growth and metastatization, its in vivo detection and quantification may allow objective diagnosis of tumor status and evaluation of treatment response. I.v. injection of cNGR-pQDs in tumorbearing mice resulted in increased quantitative contrast, comprising increased longitudinal relaxation rate and decreased proton visibility, in the tumor rim but not in tumor core or muscle tissue. This showed that cNGR-pQDs allow in vivo quantification and accurate localization of angiogenic activity. MRI results were validated using ex vivo two-photon laser scanning microscopy (TPLSM), which showed that cNGR-pQDs were primarily located on the surface of tumor endothelial cells and to a lesser extent in the vessel lumen. In contrast, unlabeled pQDs were not or only sparsely detected with both MRI and TPLSM, supporting a high specificity of cNGR-pQDs for angiogenic tumor vasculature. [Cancer Res 2008;68(18):7676-83]

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confidence: 90%

Quantitative Molecular Magnetic Resonance Imaging of Tumor Angiogenesis Using cNGR-Labeled Paramagnetic Quantum Dots

et al. 2008

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“…PS translocates from the inner to the outer leaflet of the cellular membrane in dying cells for triggering phagocytosis 63) . Annexin A5 functionalized quantum dots with improved MR sensitivity were reported by Prinzen et al 64) . MR sensitivity of these particles was enhanced by increasing the number of Gd per particle using a lysine based biotinylated construct (AnxA5-QdGd-wedge) designed to incorporate up to eight Gd-DTPA complexes.…”

Section: Quantum Dots Coated With Magnetic Shellmentioning

confidence: 86%

Luminescent and Magnetic Nanoparticulates as Biomarkers

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Brown

et al. 2010

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“…In the data presented here, however, photodegradation was not observed to hamper experiments significantly. Interestingly, quantum dots are hardly vulnerable to photobleaching, 33 making them ideal candidates for more lengthy in vivo measurements.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, 3-D imaging of the arterial wall might be possible, especially when the stability and accuracy of triggering on blood pressure is optimized. In addition, it can be used as a platform for in vivo testing and validation of novel molecular agents ͑e.g., targeted quantum dots 33 ͒ as it provides detailed information regarding the behavior of such agents in large arteries in vivo. We showed this tool to be very suitable for CNA35-mediated in vivo collagen imaging.…”

Section: Journal Of Biomedical Opticsmentioning

confidence: 99%

In vivo high-resolution structural imaging of large arteries in small rodents using two-photon laser scanning microscopy

et al. 2010

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Document VersionPublisher's PDF, also known as Version of Record (includes final page, issue and volume numbers) Please check the document version of this publication:• A submitted manuscript is the author's version of the article upon submission and before peer-review. There can be important differences between the submitted version and the official published version of record. People interested in the research are advised to contact the author for the final version of the publication, or visit the DOI to the publisher's website.• The final author version and the galley proof are versions of the publication after peer review.• The final published version features the final layout of the paper including the volume, issue and page numbers. Link to publicationCitation for published version (APA): Megens, R. T. A., Reitsma, S., Prinzen, L., Oude Egbrink, M. G. A., Engels, W., Leenders, P. J. A., ... Zandvoort, van, M. (2010). In vivo high-resolution structural imaging of large arteries in small rodents using twophoton laser scanning microscopy. Journal of Biomedical Optics, 15(1), 011108-1/10. DOI: 10.1117/1.3281672 General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal ? Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract. In vivo ͑molecular͒ imaging of the vessel wall of large arteries at subcellular resolution is crucial for unraveling vascular pathophysiology. We previously showed the applicability of two-photon laser scanning microscopy ͑TPLSM͒ in mounted arteries ex vivo. However, in vivo TPLSM has thus far suffered from in-frame and betweenframe motion artifacts due to arterial movement with cardiac and respiratory activity. Now, motion artifacts are suppressed by accelerated image acquisition triggered on cardiac and respiratory activity. In vivo TPLSM is performed on rat renal and mouse carotid arteries, both surgically exposed and labeled fluorescently ͑cell nuclei, elastin, and collagen͒. The use of short acquisition times consistently limit in-frame motion artifacts. Additionally, triggered imaging reduces between-frame artifacts. Indeed, structures in the vessel wall ͑cell nuclei, elastic laminae͒ can be imaged at subcellular resolution. In mechanically damaged carotid arteries, even the subendothelial collagen sheet ͑ϳ1 m͒ is visualized using collagentargeted quantum dots. We demonstrate stable in vivo imaging of large...

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Optical and Magnetic Resonance Imaging of Cell Death and Platelet Activation Using Annexin A5-Functionalized Quantum Dots

Cited by 152 publications

References 39 publications

Quantitative Molecular Magnetic Resonance Imaging of Tumor Angiogenesis Using cNGR-Labeled Paramagnetic Quantum Dots

Quantitative Molecular Magnetic Resonance Imaging of Tumor Angiogenesis Using cNGR-Labeled Paramagnetic Quantum Dots

Luminescent and Magnetic Nanoparticulates as Biomarkers

In vivo high-resolution structural imaging of large arteries in small rodents using two-photon laser scanning microscopy

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