Optical and Mechanical Properties of Self-Repairing Pectin Biopolymers

Pierce, Aidan; Liu, Betty S.; Liao, Matthew; Wagner, Willi L.; Khalil, Hassan A.; Chen, Zi; Ackermann, Maximilian; Mentzer, Steven J.

doi:10.3390/polym14071345

Cited by 6 publications

(4 citation statements)

References 43 publications

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“…The low-profile pectin film was previously shown to be translucent, flexible, fracture resistant, and bioabsorbable. 10 , 15 , 23 , 28 , 29 Here, we showed that the pectin film was also rapidly and strongly adherent to the corneal surface―effectively sealing corneal incisions. The strongly adherent pectin was also used for en face harvest of the corneal epithelium.…”

Section: Discussionmentioning

confidence: 74%

Biomechanics of a Plant-Derived Sealant for Corneal Injuries

Liu

Liao

Wagner

et al. 2023

Trans. Vis. Sci. Tech.

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Purpose The corneal epithelium has a glycocalyx composed of membrane-associated glycoproteins, mucins, and galactin-3. Similar to the glycocalyx in visceral tissues, the corneal glycocalyx functions to limit fluid loss and minimize frictional forces. Recently, the plant-derived heteropolysaccharide pectin has been shown to physically entangle with the visceral organ glycocalyx. The ability of pectin to entangle with the corneal epithelium is unknown. Methods To explore the potential role of pectin as a corneal bioadhesive, we assessed the adhesive characteristics of pectin films in a bovine globe model. Results Pectin film was flexible, translucent, and low profile (80 µm thick). Molded in tape form, pectin films were significantly more adherent to the bovine cornea than control biopolymers of nanocellulose fibers, sodium hyaluronate, and carboxymethyl cellulose ( P < 0.05). Adhesion strength was near maximal within seconds of contact. Compatible with wound closure under tension, the relative adhesion strength was greatest at a peel angle less than 45 degrees. The corneal incisions sealed with pectin film were resistant to anterior chamber pressure fluctuations ranging from negative 51.3 ± 8.9 mm Hg to positive 214 ± 68.6 mm Hg. Consistent with these findings, scanning electron microscopy demonstrated a low-profile film densely adherent to the bovine cornea. Finally, the adhesion of the pectin films facilitated the en face harvest of the corneal epithelium without physical dissection or enzymatic digestion. Conclusions We conclude that pectin films strongly adhere to the corneal glycocalyx. Translational Relevance The plant-derived pectin biopolymer provides potential utility for corneal wound healing as well as targeted drug delivery.

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Section: Discussionmentioning

confidence: 74%

Biomechanics of a Plant-Derived Sealant for Corneal Injuries

Liu

Liao

Wagner

et al. 2023

Trans. Vis. Sci. Tech.

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“…Finally, our studies highlight the multi-functional potential of the pectin biopolymer. In previous work, we have shown that pectin films strongly bind to the surface glycocalyx of visceral organs with a mechanism of branched chain entanglement [ 14 , 41 , 42 ]. The strong adhesivity has led to a proposed function of pectin films as a sealant for visceral organ injuries.…”

Section: Discussionmentioning

confidence: 99%

Active Loading of Pectin Hydrogels for Targeted Drug Delivery

et al. 2022

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Hydrogels provide a promising method for the targeted delivery of protein drugs. Loading the protein drug into the hydrogel free volume can be challenging due to limited quantities of the drug (e.g., growth factor) and complex physicochemical properties of the hydrogel. Here, we investigated both passive and active loading of the heteropolysaccharide hydrogel pectin. Passive loading of glass phase pectin films was evaluated by contact angles and fractional thickness of the pectin films. Four pectin sources demonstrated mean contact angles of 88° with water and 122° with pleural fluid (p < 0.05). Slow kinetics and evaporative losses precluded passive loading. In contrast, active loading of the translucent pectin films was evaluated with the colorimetric tracer methylene blue. Active loading parameters were systematically varied and recorded at 500 points/s. The distribution of the tracer was evaluated by image morphometry. Active loading of the tracer into the pectin films required the optimization of probe velocity, compression force, and contact time. We conclude that active loading using pectin-specific conditions is required for the efficient embedding of low viscosity liquids into pectin hydrogels.

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“…Pectin is among the most complex polysaccharides [64], being known for its unique chemical and functional characteristics [65]. It comprises approximately one-third of the dry substance in the cell walls of higher plants [66], particularly around actively dividing cells, soft plant tissues, cell corners, and middle lamella [64], and is responsible for intercellular adhesion and contributing to the overall plant structure and firmness [67].…”

Section: Pectin-based Vmasmentioning

confidence: 99%

A Comprehensive Review of Plant-Based Biopolymers as Viscosity-Modifying Admixtures in Cement-Based Materials

Boutouam,

Hayek,

Bouarab

et al. 2024

Applied Sciences

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As the construction industry is facing the challenge of meeting the ever-increasing demand for environmentally friendly and durable concrete, the role of viscosity-modifying admixtures (VMAs) has become increasingly essential to improve the rheological properties, stability, and mechanical properties of concrete. Additionally, natural polymers are ever evolving, offering multiple opportunities for innovative applications and sustainable solutions. This comprehensive review delves into the historical context and classifications of VMAs, accentuating their impact in enhancing the rheological properties, stability, and mechanical properties of concrete. Emphasis is placed on the environmental impact of synthetic VMAs, promoting the exploration of sustainable alternatives derived from plant-based biopolymers. Indeed, biopolymers, such as cellulose, starch, alginate, pectin, and carrageenan are considered in this paper, focusing on understanding their efficacy in improving concrete properties while enhancing the environmental sustainability within the concrete.

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Optical and Mechanical Properties of Self-Repairing Pectin Biopolymers

Cited by 6 publications

References 43 publications

Biomechanics of a Plant-Derived Sealant for Corneal Injuries

Biomechanics of a Plant-Derived Sealant for Corneal Injuries

Active Loading of Pectin Hydrogels for Targeted Drug Delivery

A Comprehensive Review of Plant-Based Biopolymers as Viscosity-Modifying Admixtures in Cement-Based Materials

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