Optical and morphological properties of infrared emitting functionalized silica nanoparticles

Iovino, G.; Malvindi, Maria Ada; Agnello, S.; Buscarino, Gianpiero; Alessi, A.; Pompa, Pier Paolo; Gelardi, F. M.

doi:10.1016/j.matchemphys.2013.08.041

Cited by 6 publications

(4 citation statements)

References 40 publications

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“…First, triethoxysilylated fluorescein derivative 1 was prepared by reaction of fluorescein isothiocyanate with 3-aminopropyl triethoxysilane (see the Supporting Information). Fluorescent nanoparticles were synthesized by the water/oil microemulsion-mediated alkoxide hydrolysis method , but using co-condensation between tetraethylorthosilicate (TEOS) and compound 1 (0.064% molar). Particles were characterized by DLS and TEM (see the Supporting Information) showing a monodisperse distribution [Dh = 79 nm; pdi (0.124); and diameter = 40 nm].…”

Section: Resultsmentioning

confidence: 99%

Unambiguous and Controlled One-Pot Synthesis of Multifunctional Silica Nanoparticles

et al. 2016

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Existing strategies for the synthesis of functional silica nanoparticles (SiNPs) rely on surface modifications followed by grafting of the organic moiety via chemoselective chemistry. Under these conditions, the introduction of several elements (e.g., ligands, drugs, fluorophores, vectors, etc.) on the same particle in a desired ratio and density is particularly difficult to achieve. We developed a method for obtaining in a single step well-defined tunable multifunctional fluorescent particles, presenting at their surface multiple ligands, covalently linked to their surface. Our strategy relies on the synthesis of hybrid bioorganic−inorganic peptide ligands, greatly simplifying the design of multifunctional nanoparticles. We demonstrated that it was possible to tune the ratio of two grafted ligands on the surface of the SiNPs simply by adjusting the relative concentration of hybrid species in the starting solution. An original fluorine nuclear magnetic resonance method was applied to the dissolved SiNPs to demonstrate our hypothesis.

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Section: Resultsmentioning

confidence: 99%

Unambiguous and Controlled One-Pot Synthesis of Multifunctional Silica Nanoparticles

et al. 2016

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“…Our first attempts to prepare Fluorescent SiNPs were based on reverse water-in-oil microemulsion-mediated metallic alkoxide hydrolysis method [ 19 , 20 ], using cyclohexane, ammonia, hexane, and Triton X-100, with co-condensation between TEOS and our hybrid fluorophore 1 (0.2% molar). First, the triethoxysilylated fluorophore was prepared by reaction of commercially available Sulfo Cyanine 5.5 NHS ester with 3-aminopropyltriethoxysilane (APTES) to yield hybrid cyanine 1 in DMSO ( Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

Design of PEGylated Three Ligands Silica Nanoparticles for Multi-Receptor Targeting

et al. 2021

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The synthesis of silica nanoparticles (SiNPs) decorated on their surface with a range of various elements (e.g., ligands, drugs, fluorophores, vectors, etc.) in a controlled ratio remains a big challenge. We have previously developed an efficient strategy to obtain in one-step, well-defined multifunctional fluorescent SiNPs displaying fluorophores and two peptides ligands as targeting elements, allowing selective detection of cancer cells. In this paper, we demonstrate that additional level of controlled multifunctionality can be achieved, getting even closer to the original concept of “magic bullet”, using solely sol–gel chemistry to achieve conjugation of PEG chains for stealth, along with three different ligands. In addition, we have answered the recurrent question of the surface ungrafting by investigating the stability of different siloxane linkages with the ERETIC Method (Electronic Reference to Access In Vivo Concentrations) by 19F NMR quantification. We also compared the efficiency of the hybrid silylated fluorophore covalent linkage in the core of the SiNP to conventional methods. Finally, the tumor-cell-targeting efficiency of these multi-ligand NPs on human endothelial cells (HUVEC or HDMEC) and mixed spheroids of human melanoma cells and HUVEC displaying different types of receptors were evaluated in vitro.

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“…1 Due to their anti-caking properties, SiO 2 NP have found application in food production, 2 and they are also used in paints and coatings, 3 microelectronic devices, 4 as well as imaging probes in biomedical sectors. 5,6 Such a widespread use of nanomaterials (NM) has raised safety concerns, arising from a possibility of their uncontrolled release from the commercial product and subsequent impact on humans and the environment. 7 Once ingested, inhaled or absorbed through the skin, NP can interact with individual cells within the body and trigger various biological and toxicological responses.…”

Section: Introductionmentioning

confidence: 99%

Changes in silica nanoparticles upon internalisation by cells: size, aggregation/agglomeration state, mass- and number-based concentrations

et al. 2018

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Monitoring the physicochemical characteristics of nanoparticles following internalisation by cells is a vital step in understanding their biological impact and toxicity. Here, the feasibility of a methodology utilising gentle enzymatic lysis of cells containing internalised particles and direct analysis of the lysates for the particle size, agglomeration state and concentration, is investigated. It is demonstrated that following internalisation, all types of studied silica particles partially agglomerate/aggregate, with the degree and rate of the observed transformation closely correlated with the initial particle surface chemistry. Several different particle populations are noted and characterised in terms of their size and concentration. Good agreement between different complementary techniques is reached in terms of the average particle diameter. Particle concentration is determined here with techniques capable of mass and number-based measurements, with limitations of approaches utilising signal conversion to equivalent particle numbers identified and discussed.

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Optical and morphological properties of infrared emitting functionalized silica nanoparticles

Cited by 6 publications

References 40 publications

Unambiguous and Controlled One-Pot Synthesis of Multifunctional Silica Nanoparticles

Unambiguous and Controlled One-Pot Synthesis of Multifunctional Silica Nanoparticles

Design of PEGylated Three Ligands Silica Nanoparticles for Multi-Receptor Targeting

Changes in silica nanoparticles upon internalisation by cells: size, aggregation/agglomeration state, mass- and number-based concentrations

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