Optical and Structural Properties of Biological Tissues under Simulated Diabetes Mellitus Conditions

Tuchina, Daria K.; Bucharskaya, Alla B.; Dyachenko, Polina A.; Дихт, Н. И.; Terentyuk, Georgy S.; Tuchin, Valery V.

doi:10.1201/9781003112099-1

Cited by 4 publications

(3 citation statements)

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“…Molecular models of iohexol, sucrose, ribose, glucose, fructose, and glycerol were used as OCAs. It should be noted that when collagen interacts with OCA for a long time, about several weeks [36], glycation of collagen protein may occur with the formation of covalent cross-links. During molecular modeling, we did not take this aspect into account, since in our case the interaction of OCA with collagen occurs within a relatively short time suitable in clinical practice.…”

Section: Molecular Modelingmentioning

confidence: 99%

Optical clearing of human skin: Molecular modeling and in vivo OCT study

Berezin,

Grabarchuk,

Lichter

et al. 2023

Journal of Biophotonics

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The results of in vivo immersion optical clearing of human skin under the action of two different optical clearing agents (OCAs), such as an aqueous sucrose solution and a radiographic contrast agent Omnipaque™ 300 (iohexol), were obtained with the use of optical coherence tomography (OCT) method. The rate of reduction of light scattering coefficient, obtained through an averaged A‐scan of the OCT image in the region of dermis within the depths from 350 to 700 μm, were determined to evaluate the efficiency of optical clearing (EOC). The correlations between the EOC and the energy of intermolecular interaction of OCAs with a fragment of collagen peptide have been established as a result of molecular modeling by quantum chemistry methods HF/STO3G/DFT/B3LYP/6—311G(d) of a number of OCAs (glycerol, iohexol, sucrose, ribose, fructose, glucose) with mimetic peptide of collagen (GPH)3.

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Section: Molecular Modelingmentioning

confidence: 99%

Optical clearing of human skin: Molecular modeling and in vivo OCT study

Berezin,

Grabarchuk,

Lichter

et al. 2023

Journal of Biophotonics

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“…Differences in skin tone which can affect the penetration of excitation wavelength and detection of fAGEs, and differences in individuals' sun exposure which may lead to fAGE photobleaching. In other studies, optical analysis of the effects of tissue glycation has been performed with techniques such as multiphoton microscopy, optoacoustic detection, and others [44][45][46][47][48][49][50][51][52][53][54]. Finally, an assay for diabetic complications based on fAGE can be complicated by differences in sugar consumption.…”

Section: Introductionmentioning

confidence: 99%

Multiphoton imaging of the monosachharide induced formation of fluorescent advanced glycation end products in tissues

Lin,

Mondal,

Lee

et al. 2023

Journal of Biophotonics

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We studied the in vitro rate of fluorescent AGEs (fAGEs) formation with multiphoton microscopy in different porcine tissues (aorta, cornea, kidney, dermis, and tendon). These tissues were treated with D‐glucose, D‐galactose, and D‐fructose, three primary monosaccharides found in human diets.We found that the use of D‐fructose resulted in the highest glycation rate, followed by D‐galactose and then D‐glucose. Moreover, compared to non‐collagen tissue constituents such as elastic fibers and cells, the rate of tissue glycation was consistently higher in collagen, suggesting that collagen is a more sensitive target for fAGE formation. However, we also found that collagen in different tissues exhibits different rates of fAGE formation, with slower rates observed in tightly packed tissues such as cornea and tendon.Our study suggests that for fAGE to be developed into a long‐term glycemic biomarker, loosely organized collagen tissues located in the proximity of vasculature may be the best targets.This article is protected by copyright. All rights reserved.

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“…Collagen is the main component of the extracellular matrix in mammals, including humans, so the structure and function of the skin depend primarily on its condition. As a person ages, the content of collagen in the dermis increases and its physical and chemical properties also change: 9 – 12 the number and strength of inter- and intramolecular cross-links increases, whereas their elasticity and ability to swell decreases, collagen fibers develop resistance to collagenase, etc. [see Figs.…”

Section: Introductionmentioning

confidence: 99%

Incremental residual polarization caused by aging in human skin

Dremin,

Zharkikh,

Lopushenko

et al. 2023

J. Biomed. Opt.

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The study of the effect of aging on the optical properties of biological tissues, in particular polarization, is important in the development of new diagnostic approaches.Aim: This work aims to provide a comprehensive analysis of the factors and mechanisms that contribute to the alteration of skin polarization properties caused by aging, using polarization-sensitive hyperspectral imaging measurements and Monte Carlo simulation.Approach: Our investigation involved both experimental studies of in vivo human skin of volunteers of different ages and computational modeling that accounted for changes in the absorption and scattering properties of the skin model. Specifically, we analyzed alterations in the degree of linear polarization (DOLP) to better understand the impact of aging on skin polarization properties.Results: A statistically significant increase in the DOLP was found for the elderly group. At the same time, there was no correlation between changes in polarization and the calculated blood volume fraction parameter for different ages. According to the simulation results, it was also found that a change in the scattering properties of biological tissues has a more significant effect on the change in polarizing light compared to the change in absorption. Conclusions:The results of the work prove that the sensitivity of polarization imaging to age-or pathological-related skin changes may be primarily due to changes in scattering, which in turn is associated with changes in the collagen fibers of the dermis. The proposed technique shows promise for in vivo non-invasive real-time assessment of age-associated skin changes and can also be extended to monitor changes associated with the development of age-related pathologies.

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Optical and Structural Properties of Biological Tissues under Simulated Diabetes Mellitus Conditions

Cited by 4 publications

References 0 publications

Optical clearing of human skin: Molecular modeling and in vivo OCT study

Optical clearing of human skin: Molecular modeling and in vivo OCT study

Multiphoton imaging of the monosachharide induced formation of fluorescent advanced glycation end products in tissues

Incremental residual polarization caused by aging in human skin

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