Optical and X-Ray Diffraction Studies of Certain Calcium Phosphates

Bale, William F.; Bonner, John F.; Hodge, Harold C.; Adler, Howard; Wreath, A.R.; Bell, R.

doi:10.1021/i560144a010

Cited by 32 publications

(23 citation statements)

References 8 publications

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“…It is noteworthy that in all of these preparations the temperature of apparent fusion of the crystals was far below their bulk melting point (1 720C for tricalcium phosphate, according to Bale et al, 1945) but in the same temperature range as for the mitochondrial dense granule ash.…”

Section: Multimilligram Samples Of a Calcium-deficientmentioning

confidence: 85%

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Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Thomas

Greenawalt

1968

The Journal of Cell Biology

106

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Isolated rat liver mitochondria were incubated in vitro under conditions supporting the massive accumulation of calcium and phosphate. Samples were embedded, thin sectioned, and examined in the electron microscope. The intramitochondrial distribution of insoluble or structure-bound mineral substances was studied by electron microscopy coupled with recently developed techniques of high resolution microincineration. As shown previously, the ion-loaded mitochondria acquire large, internal granules which have inherent electron opacity indicative of high mineral content. Study of ash patterns in preselected areas of sections directly confirmed the high mineral content of the granules, and the appearance of the residues was consistent with the copresence in the granules of some organic material. Other mitochondrial structures were almost devoid of mineral. Thin sections of unincubated control mitochondria also were incinerated. They were found to contain appreciable amounts of intrinsic mineral, seemingly associated with membranes. The normal, dense matrix granules commonly seen in unaltered mitochondria could be seen in intact sections of these control preparations, but after burning no definite correspondence of any ash to the granules could be demonstrated. The normal granules perhaps do not contain mineral. Heating experiments on ash patterns of all the preparations demonstrated the thermal stability and crystallizability of the ash. The crystallized ash of the in vitro-produced dense granules was tentatively shown by electron diffraction to be 3-tricalcium phosphate (whitlockite). This, together with evidence from the literature, suggests that the original, noncrystalline mineral may be a colloidal, subcrystalline precursor of calcium-deficient hydroxyapatite. Experiments were performed on synthetic calcium phosphates for comparison. Other possible applications of the microincineration techniques are briefly discussed.

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Section: Multimilligram Samples Of a Calcium-deficientmentioning

confidence: 85%

“…The bulk melting point of tricalcium phosphate is 1720 0 C (Bale et al, 1945). In defense of the identification, electron microscopic preparations of verified calcium phosphates showed similar behavior.…”

mentioning

confidence: 90%

Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Thomas

Greenawalt

1968

The Journal of Cell Biology

106

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“…The image in Fig. 1 shows a needle morphology with an average length of 55 ± 9 nm and average diameter equal to 10 ± 1 nm [21,22]. Characterization by FTIR showed the characteristic functional groups corresponding to HA; vibration of the hydroxyl group was observed around 3700 cm -1 , while the bands at 1429, 1089-1035, 962, 603, 565, and 477 cm -1 correspond to the vibration modes of the phosphate group (PO 4 -3 ) (Fig.…”

Section: Synthesis and Characterization Of Ha And Hdpe-ha Compositesmentioning

confidence: 99%

Biocompatibility studies of HDPE–HA composites with different HA content

Hermán

González²,

Noris-Suárez

et al. 2015

Polym. Bull.

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High-density polyethylene-hydroxyapatite (HDPE-HA) composites with different filler content (5-20 %) were synthesized by in situ ethylene polymerization. Good filler dispersion was observed without formation of agglomerates. Osteoblast cell behavior in HDPE-HA composites was evaluated in terms of adhesion, alkaline phosphatase activity and proliferation. Fluorescence and scanning electron microscopy results showed good cell adhesion and proliferation, and extensive filopodium-like protrusion connected to hydroxyapatite particles.

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“…For the Ti-6Al-7Nb titanium alloy, two phases were found: the phase of titanium and that of aluminum alloy (PDF #04-004-9156), where the main peak positions on the XRD pattern were detected at 35 No signals for the crystalline Ca-based phase were identified. The calcium phosphate was determined where the samples were anodized in the 0.1 M Ca(H2PO2)2 solution with ceramic particles of calcium phosphate (Ca3(PO4)2 (PDF #00-003-0348), and the XRD pattern is presented in Figure 7 [33].…”

Section: Resultsmentioning

confidence: 99%

Analysis of the Calcium Phosphate-Based Hybrid Layer Formed on a Ti-6Al-7Nb Alloy to Enhance the Ossseointegration Process

et al. 2020

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This paper reports on hybrid, bioactive ceramic Ca-P-based coating formation on a Ti-6Al-7Nb alloy substrate to enhance the osseointegration process. The Ti alloy was anodized in a Ca3(PO4)2 suspension and then the additional layer was formed by the sol-gel technique to obtain a mixture of the calcium phosphate compounds. The oxide layer was porous and additional ceramic particles were formed after sol-gel treatment (scanning electron microscopy analysis coupled with energy-dispersive x-ray spectroscopy). The ceramic particles were formed on some parts of the oxide layer and did not completely fill the pores. The layer thickness of the anodized Ti alloy was comprised between 3.01 and 5.03 µm and increased to 7.52–12.30 µm after the formation of an additional layer. Post-treatment of the anodized Ti alloys caused a decrease in surface roughness, and the layer became strongly hydrophilic. Crystalline phase analysis (X-ray diffraction, XRD) showed that the hybrid layer was composed of TiO2 (anatase), Ca3(PO4)2, Ca10(PO4)6(OH)2 and a partially amorphous phase; thus, the layer was also analyzed by Raman spectroscopy. The hybrid layer showed worse adhesion to the substrate than the anodized layer only; however, the coating was not brittle, and the first delamination of the layer was determined at 1.84 ± 0.11 N during scratch-test measurement. The hybrid coating was favorable for collagen type I and lactoferrin adsorption, strongly influencing the proliferation of osteoblast-like MG-63 cells. The coatings were cytocompatible and may find applications in formation of the functional layers on long-term implants’ surface after.

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Optical and X-Ray Diffraction Studies of Certain Calcium Phosphates

Cited by 32 publications

References 8 publications

Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Microincineration, Electron Microscopy, and Electron Diffraction of Calcium Phosphate-Loaded Mitochondria

Biocompatibility studies of HDPE–HA composites with different HA content

Analysis of the Calcium Phosphate-Based Hybrid Layer Formed on a Ti-6Al-7Nb Alloy to Enhance the Ossseointegration Process

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