Optical approach to the salivary pellicle

Baek, Jae Ho; Krasieva, Tatiana B.; Tang, Shuo; Ahn, Yeh‐Chan; Kim, Chang Soo; Vu, Diana; Chen, Zhongping; Wilder‐Smith, Petra

doi:10.1117/1.3158994

Cited by 20 publications

(31 citation statements)

References 24 publications

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“…Although some research has been reported in ophthalmologic, dermatologic, gastrointestinal, gynecological, cardiac, and other OCT applications, (27) the little research that has been reported in the oral cavity has focused predominantly on oral malignancy, periodontal disease, and caries (28–32). Our preliminary data using OCT and a simulated GERD model in extracted teeth show early changes in enamel thickness due to chronic GERD that are potentially greater than 50 μm, whereas the variability of the technique between repeated measurements at maximum resolution levels is below 10% (33). In this study we used OCT to quantify tooth substance loss as well as changes in optical reflectance and absorbance of dental hard tissues as measures of demineralization before and after 3 weeks of esomeprazole (20 mg b.i.d.)…”

Section: Introductionmentioning

confidence: 83%

“…Two processes contribute to this phenomenon. First, back reflections occurring at the pore–enamel interfaces due to abrupt changes of the optical refractive index and, second, an effective reduction in scattering when light is traveling virtually without being deviated through the space inside the pores (33,40). This effect ensures that light coming from deeper regions within the sample reaches the OCT detection system.…”

Section: Discussionmentioning

confidence: 99%

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Quantification of Dental Erosions in Patients With GERD Using Optical Coherence Tomography Before and After Double-Blind, Randomized Treatment With Esomeprazole or Placebo

Wilder‐Smith¹,

et al. 2009

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Objectives Dental erosion, the chemical dissolution of enamel without bacterial involvement, is a rarely reported manifestation of gastroesophageal reflux disease (GERD), as well as of recurrent vomiting and dietary habits. It leads to loss of tooth substance, hypersensitivity, functional impairment, and even tooth fracture. To date, dental erosions have been assessed using only very basic visual methods, and no evidence-based guidelines or studies exist regarding the prevention or treatment of GERD-related dental erosions. Methods In this randomized, double-blind study, we used optical coherence tomography (OCT) to quantify dental tissue demineralization and enamel loss before and after 3 weeks of acid-suppressive treatment with esomeprazole 20 mg b.i.d. or placebo in 30 patients presenting to the Berne University Dental Clinic with advanced dental erosions and abnormal acid exposure by 24-h esophageal pH manometry (defined as >4% of the 24-h period with pH < 4). Enamel thickness, reflectivity, and absorbance as measures of demineralization were quantified by OCT before and after therapy at identical localizations on teeth with most severe visible erosions as well as several other predefined changes in teeth. Results The mean±s.e.m. decrease of enamel thickness of all teeth before and after treatment at the site of maximum exposure was 7.2±0.16 μm with esomeprazole and 15.25±0.17 μm with placebo (P =0.013), representing a loss of 0.3% and 0.8% of the total enamel thickness, respectively. The change in optical reflectivity to a depth of 25 μm after treatment was −1.122 ±0.769 dB with esomeprazole and +2.059±0.534 dB with placebo (P = 0.012), with increased reflectivity signifying demineralization. Conclusions OCT non-invasively detected and quantified significantly diminished progression of dental tissue demineralization and enamel loss after only 3 weeks of treatment with esomeprazole 20 mg b.i.d. vs. placebo. This suggests that esomeprazole may be useful in counteracting progression of GERD-related dental erosions. Further validation of preventative treatment regimens using this sensitive detection method is required, including longer follow-up and correlation with quantitative reflux measures.

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Section: Introductionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Quantification of Dental Erosions in Patients With GERD Using Optical Coherence Tomography Before and After Double-Blind, Randomized Treatment With Esomeprazole or Placebo

Wilder‐Smith¹,

et al. 2009

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“…In order to compare the results and to improve the resolution and specificity of images, an optical coherence microscope (OCM) was used. Salivary pellicle islands were visible in the samples incubated in saliva, which grow into complexes completely covering the enamel surface [ 43 ]. The aim of the next study was to evaluate the retention of the biofilm around orthodontic hooks depending on the ligaturing method using OCT and microbiological samples.…”

Section: The Short History Of Oct In Dentistrymentioning

confidence: 99%

The Use of Optical Coherence Tomography in Dental Diagnostics: A State-of-the-Art Review

Machoy

Seeliger

Szyszka-Sommerfeld

et al. 2017

Journal of Healthcare Engineering

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Optical coherence tomography provides sections of tissues in a noncontact and noninvasive manner. The device measures the time delay and intensity of the light scattered or reflected from biological tissues, which results in tomographic imaging of their internal structure. This is achieved by scanning tissues at a resolution ranging from 1 to 15 μm. OCT enables real-time in situ imaging of tissues without the need for biopsy, histological procedures, or the use of X-rays, so it can be used in many fields of medicine. Its properties are not only particularly used in ophthalmology, in the diagnosis of all layers of the retina, but also increasingly in cardiology, gastroenterology, pulmonology, oncology, and dermatology. The basic properties of OCT, that is, noninvasiveness and low wattage of the used light, have also been appreciated in analytical technology by conservators, who use it to identify the quality and age of paintings, ceramics, or glass. Recently, the OCT technique of visualization is being tested in different fields of dentistry, which is depicted in the article.

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“…Its primary mode of action is hypothesized to be through chemical desiccation or dehydration, leaving a denatured and destabilized biofilm which can then be removed by irrigation. In this pilot study, 3D Nonlinear Optical Microscopy imaging (NLOM) was used to generate non-contact high-resolution images of the biofilm and tooth surface [ 15 – 17 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring Mechanisms of Biofilm Removal

et al. 2016

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ObjectiveThe goal of this study was to evaluate the effects of a novel anti-plaque formulation on oral biofilm removal. Specific aim was to elucidate the role of 2 potentially complementary mechanisms on dental biofilm removal using EPIEN Dental Debriding Solution (EDDS) like desiccating action leading to denaturation and destabilization of plaque and mechanical removal of destabilized plaque through forceful rinsing actionMaterials and Methods25 extracted teeth, after routine debriding and cleaning, underwent standard biofilm incubation model over 4 days. Then samples were randomly divided into 5 groups of 5 teeth each, treated and stained with GUM®Red-Cote® plaque disclosing solution and imaged. Samples were subsequently treated with HYBENX® Oral Decontaminant. Group 1 samples were treated with a standardized “static” water dip exposure following biofilm incubation. Samples in Group 2 were given a standardized “dynamic” exposure to a dental high pressure air/water syringe for 20 s. Group 3 samples were exposed to a standardized “static” application of test agent (30 s dip rinse) followed by a standardized “static” water rinse (30 s dip rinse). Samples in Group 4 were given both the standardized “static” application of test formulation followed by the standardized “dynamic” exposure to a dental high pressure air/water syringe. Finally, samples in Group 5 were treated with a standardized “dynamic” application of test agent (20 s high pressure syringe at 10 ml/s) followed by the standardized “dynamic” exposure to a dental high pressure air/water syringe.ResultsThe MPM images demonstrated that the water dip treatment resulted in the persistence of an almost continuous thick layer of biofilm coverage on the tooth surface. Similarly, test agent dip treatment followed by water dip only removed a few patches of biofilm, with the majority of the tooth surface remaining covered by an otherwise continuous layer of biofilm. Samples exposed to air/water spray alone showed some disruption of the biofilm, leaving residual patches of biofilm that varied considerably in size. Test agent dip treatment followed by air/water spray broke up the continuous layer of biofilm leaving only very small, thin scattered islands of biofilm. Finally, the dynamic test agent spray followed by air/water spray removed the biofilm almost entirely, with evidence of only very few small, thin residual biofilm islands.ConclusionThese studies demonstrate that test agent desiccant effect alone causes some disruption of dental biofilm. Additional dynamic rinsing is needed to achieve complete removal of dental biofilm.

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Optical approach to the salivary pellicle

Cited by 20 publications

References 24 publications

Quantification of Dental Erosions in Patients With GERD Using Optical Coherence Tomography Before and After Double-Blind, Randomized Treatment With Esomeprazole or Placebo

Quantification of Dental Erosions in Patients With GERD Using Optical Coherence Tomography Before and After Double-Blind, Randomized Treatment With Esomeprazole or Placebo

The Use of Optical Coherence Tomography in Dental Diagnostics: A State-of-the-Art Review

Exploring Mechanisms of Biofilm Removal

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