“…MATERIALS AND METHODSSurgical specimens of formalin-fixed tissue of HSCR patients were subjected to this study. First, the Raman spectral data of the mucosa were comprehensively collected by using a home-built Raman microscopy system6 . In brief, the Raman microscopy system consists of a laser source (785 nm, 08-NLD, Cobolt, Solna, Sweden), a spectrometer (M10-TP, 1200/mm, 850 nm blazed, Bunkoukeiki, Tokyo, Japan), an EM-CCD (iXon3-888, ANDOR TECHNOLOGY, Belfast, UK).…”