Optical Cell with a Temperature-Control Unit for a Vacuum-Ultraviolet Circular Dichroism Spectrophotometer

Matsuo, Koichi; Sakai, Kenichi; Matsushima, Yosuke; Fukuyama, Takayuki; Gekko, Kunihiko

doi:10.2116/analsci.19.129

Cited by 65 publications

(73 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Details of the spectrophotometer and optical cell used are available elsewhere [21,28]. The path length of the CaF 2 optical cell was adjusted using a Teflon spacer to 10 μm for the measurements from 200 to 172 nm, while no spacer was used for measurements below 172 nm in order to reduce the absorption of light by water.…”

Section: Vuvcd Measurementsmentioning

confidence: 99%

“…Some previous studies have found that the VUVCD spectra are sensitive to the equilibrium structures of monosaccharides in aqueous solution, such as the gauche (G) and trans (T) conformations of the hydroxylmethyl group at C-5, and the α-and β-anomer configurations of the hydroxyl group at C-1 [3,17,24,25]. However, since the pairwise relationships between the structures and VUVCD of monosaccharides remained unclear from these experimental results, we have recently measured the VUVCD spectra of various methyl aldopyranosides including methyl α-D-glucopyranosides in aqueous solution [19,20] using a synchrotron-radiation (SR) VUVCD spectrophotometer at the Hiroshima Synchrotron Radiation Center [21,28]. Using the time-dependent density functional theory (TDDFT) and molecular dynamics (MD) simulations involving explicit water molecules [15,35,40], we theoretically revealed that the GT and GG rotamers exhibited negative and positive CD around 170 nm, respectively, while the α-and β-anomer configurations showed negative and positive CD around 160 nm, respectively [19,20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vacuum-ultraviolet circular dichroism study of oligosaccharides using a synchrotron-radiation spectrophotometer

Matsuo

2017

BSI

Self Cite

View full text Add to dashboard Cite

Abstract. Vacuum-ultraviolet circular dichroism (VUVCD) spectra of malto-, laminari-, isomalto-, and cello-oligosaccharide series and their corresponding polysaccharides (laminarin and dextran) were measured from 200 to 168 nm in aqueous solution at 25°C using a synchrotron-radiation VUVCD spectrophotometer. Disaccharides exhibited markedly different CD spectra depending on the types of glycosidic linkages, and the CD spectra of each oligosaccharide series (with the exception of the isomalto-oligosaccharide series) varied with the chain length below 190 nm while retaining the spectral shape of the constituent disaccharide. These results indicate that the basic structures of oligosaccharides were greatly affected by the configurations of their constituent disaccharides, which had unique torsion angles restricted by the intramolecular hydrogen bonds between glucose units. Based on comparisons between the experimental and theoretical data, we suggest that the chain-length dependence of CD above 180 nm reflects the backbone structure of oligosaccharides (e.g., helical structures), while those below 180 nm are influenced by other factors associated with higher-energy chromophores such as the hydroxyl groups. The reported comprehensive VUVCD spectra provide basic information for understanding the complicated structures of oligosaccharides in aqueous solution that can be used in their theoretical assignments.

show abstract

Section: Vuvcd Measurementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vacuum-ultraviolet circular dichroism study of oligosaccharides using a synchrotron-radiation spectrophotometer

Matsuo

2017

BSI

Self Cite

View full text Add to dashboard Cite

show abstract

“…VUVCD measurements were performed with a synchrotron-radiation VUVCD spectrophotometer at the Hiroshima Synchrotron Radiation Center (HiSOR). Details of the spectrophotometer and the optical cell with a temperature-control unit are available elsewhere [11,12]. The optical cell with CaF 2 windows was used, and no spacer was used in order to reduce the light absorption by water at lower wavelengths.…”

Section: Methodsmentioning

confidence: 99%

“…CD spectra in VUV region below 190 nm can be measured accurately by a VUVCD spectrophotometer using synchrotron radiation as an intense light source [11,12]. With the aid of the VUVCD spectroscopy, a contribution of gelatin to the CD spectra can be separated from that of sugars and χ was shown to be determined from ellipticity θ at a wavelength of 198 nm.…”

Section: Introductionmentioning

confidence: 99%

Effect of sugars on aging of gelatin gels by vacuum-ultraviolet circular dichroism and rheological measurements

et al. 2014

Self Cite

View full text Add to dashboard Cite

Many food products based on gelatin contain sugar. Although the effect of sugars on physical properties of gelatin gels has been intensively investigated, aging of gelatin gels containing sugars has not been studied in detail. In the present study, vacuum-ultraviolet circular dichroism (VUVCD) was applied to the study of the aging of gelatin gels with and without sugars and the aging process measured by VUVCD was compared with that measured by rheology and polarimetry. The storage modulus G' and reduced ellipticity θ/θ 0 in the aging process were correlated with each other, indicating VUVCD is a useful tool for detecting the conformation change of gelatin molecules. On the other hand, the behavior of optical rotation Δα was different with that of either G' or θ/θ 0 , suggesting that the optical rotation does not represent the conformation change accurately in the case of gelatin gels containing sugars. The relationship between G' and a fraction χ of helix amount estimated from VUVCD data showed that the critical helix fraction χ c for gelation is slightly increased by the addition of sugars, suggesting a change in the network structure.

show abstract

“…The details of the optical devices, sample cell, and data acquisition of the spectrophotometer were described previously. [30][31][32][33] The enzyme (141 μM) was prepared in buffer containing 10 mM sodium phosphate, pH 7.4. The secondary structure contents of ChiW-SLHd were estimated using the SELCON3 program 34) and the VUVCD spectra of the 31 reference proteins with known X-ray structures.…”

Section: Genetic Analysismentioning

confidence: 99%

Overexpression, purification, and characterization of Paenibacillus cell surface-expressed chitinase ChiW with two catalytic domains

Itoh

Sugimoto

Hibi

et al. 2014

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

Paenibacillus sp. strain FPU-7 produces several different chitinases and effectively hydrolyzes robust chitin. Among the P. FPU-7 chitinases, ChiW, a novel monomeric chitinase with a molecular mass of 150 kDa, is expressed as a cell surface molecule. Here, we report that active ChiW lacking the anchoring domains in the N-terminus was successfully overproduced in Escherichia coli and purified to homogeneity. The two catalytic domains at the C-terminal region were classified as typical glycoside hydrolase family 18 chitinases, whereas the N-terminal region showed no sequence similarity to other known proteins. The vacuum-ultraviolet circular dichroism spectrum of the enzyme strongly suggested the presence of a β-stranded-rich structure in the N-terminus. Its biochemical properties were also characterized. Various insoluble chitins were hydrolyzed to N,N’-diacetyl-D-chitobiose as the final product. Based on amino acid sequence similarities and site-directed mutagenesis, Glu691 and Glu1177 in the two GH-18 domains were identified as catalytic residues.

show abstract

Optical Cell with a Temperature-Control Unit for a Vacuum-Ultraviolet Circular Dichroism Spectrophotometer

Cited by 65 publications

References 10 publications

Vacuum-ultraviolet circular dichroism study of oligosaccharides using a synchrotron-radiation spectrophotometer

Vacuum-ultraviolet circular dichroism study of oligosaccharides using a synchrotron-radiation spectrophotometer

Effect of sugars on aging of gelatin gels by vacuum-ultraviolet circular dichroism and rheological measurements

Overexpression, purification, and characterization of Paenibacillus cell surface-expressed chitinase ChiW with two catalytic domains

Contact Info

Product

Resources

About