Optical Clearing and Tissue Imaging

Oliveira, Luís; Tuchin, Valery V.

doi:10.1007/978-3-030-33055-2_7

Cited by 5 publications

(4 citation statements)

References 110 publications

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“…However, their probing depth and image contrast in vivo are limited by the absorption and the strong scattering properties of the cutaneous tissues 1,4 . Biological tissue optical clearing (OC) methods 5,6 have been widely investigated to reduce skin scattering ex vivo and in vivo. Nowadays, there are many examples of using OC technique to improve the contrast of optical imaging and, in particular, its application for skin cancer characterization 7 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use

Blondel¹,

Amouroux²,

Tuchin³

et al. 2023

Dynamics and Fluctuations in Biomedical Photonics XX

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The clinical use of optical methods for in vivo skin imaging is limited by skin strong scattering properties, which reduce image contrast and probing depth. The efficiency of optical methods can be improved by optical clearing (OC). However, for the use of optical clearing agents (OCAs) in a clinical setting, compliance with acceptable non-toxic concentrations is required. Optical clearing of in vivo human skin, combined with physical and chemical methods to enhance skin permeability to OCAs, was performed to determine the clearing-effectiveness of biocompatible OCAs using Line-field Confocal Optical Coherence Tomography (LC-OCT) imaging. Nine types of OCAs mixtures were used in association with dermabrasion and sonophoresis for optical clearing protocol on three volunteers hand skin. From 3D images obtained every five minutes for 40 minutes, the intensity and contrast parameters were extracted to assess their changes during the clearing process and evaluate each OCAs mixture's clearing efficacy. LC-OCT images average intensity and contrast increased over the entire skin depth with all OCAs. The best image contrast and intensity improvement was observed using the Polyethylene Glycol, Oleic Acid and Propylene Glycol mixture. Complex OCAs featuring reduced component concentrations that meet drug regulation-established biocompatibility requirements were developed and proved to induce significant skin tissues clearing. By allowing deeper observations and higher contrast, such OCAs in combination with physical and chemical permeation enhancers may improve LC-OCT diagnostic efficacy.

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Section: Introductionmentioning

confidence: 99%

Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use

Blondel¹,

Amouroux²,

Tuchin³

et al. 2023

Dynamics and Fluctuations in Biomedical Photonics XX

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“…1 Now, a widely investigated method to increase the refractive index of the interstitial fluid and thus reduce scattering is tissue optical clearing (OC). 10,11 It is based on the use of chemical agents whose refractive indices are close to those of the structural components of biological tissue. Due to their immersion as a result of surface application or injection into biological tissue, they cause partial dehydration of the skin, and then replace the interstitial fluid.…”

Section: Introductionmentioning

confidence: 99%

“…The main reason for the strong scattering of biological tissues is the difference in the refractive indices (RI) of the tissue components and the interstitial fluid 1 . Now, a widely investigated method to increase the refractive index of the interstitial fluid and thus reduce scattering is tissue optical clearing (OC) 10 , 11 . It is based on the use of chemical agents whose refractive indices are close to those of the structural components of biological tissue.…”

Section: Introductionmentioning

confidence: 99%

“…At the moment, there are many examples of using an OC technique to improve the contrast of OCT skin images in vivo 10 , 11 , 14 – 16 and, in particular, its application for skin cancer characterization. 17 However, when translating these methods into clinical use, there is a need to comply with established regulations, including the use of chemicals on healthy and pathologically changed skin of patients.…”

Section: Introductionmentioning

confidence: 99%

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In vivo skin optical clearing efficacy quantification of clinically compatible agents using line-field confocal optical coherence tomography

et al. 2023

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Significance: The clinical use of optical methods for in vivo skin imaging is limited by skin strong scattering properties, which reduce image contrast and probing depth. The efficiency of optical methods can be improved by optical clearing (OC). However, for the use of OC agents (OCAs) in a clinical setting, compliance with acceptable non-toxic concentrations is required.Aim: OC of in vivo human skin, combined with physical and chemical methods to enhance skin permeability to OCAs, was performed to determine the clearing-effectiveness of biocompatible OCAs using line-field confocal optical coherence tomography (LC-OCT) imaging.Approach: Nine types of OCAs mixtures were used in association with dermabrasion and sonophoresis for OC protocol on three volunteers hand skin. From 3D images obtained every 5 min for 40 min, the intensity and contrast parameters were extracted to assess their changes during the clearing process and evaluate each OCAs mixture's clearing efficacy. Results:The LC-OCT images average intensity and contrast increased over the entire skin depth with all OCAs. The best image contrast and intensity improvement was observed using the polyethylene glycol, oleic acid, and propylene glycol mixture.Conclusions: Complex OCAs featuring reduced component concentrations that meet drug regulation-established biocompatibility requirements were developed and proved to induce significant skin tissues clearing. By allowing deeper observations and higher contrast, such OCAs in combination with physical and chemical permeation enhancers may improve LC-OCT diagnostic efficacy.

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Estimation of Scattering Properties Modifications Caused by In Vivo Human Skin Optical Clearing Using Line‐Field Confocal Optical Coherence Tomography

Zaytsev,

Waszczuk,

Ogien

et al. 2024

Journal of Biophotonics

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The image contrast and probing depth of optical methods applied to in vivo skin could be improved by reducing skin scattering using the optical clearing method. The aim of this study was to quantify, from line‐field confocal optical coherence tomography (LC‐OCT) 3D images, the modifications of skin scattering properties in vivo during optical clearing. Nine mixtures of optical clearing agents were used in combination with physical and chemical permeation enhancers on the human skin of three healthy volunteers. Scattering coefficient and anisotropy factor of the epidermis and the upper dermis were estimated from the 3D LC‐OCT images of skin using an exponential decay model of the in‐depth intensity profile. We were able to demonstrate a decrease in epidermal scattering (down to 33%) related to optical clearing, with the best results obtained by a mixture of polyethylene glycol, oleic acid, and propylene glycol.

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Optical Clearing and Tissue Imaging

Cited by 5 publications

References 110 publications

Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use

Characterization of human skin optical clearing based on confocal optical coherence tomography imaging and optical clearing agents for clinical use

In vivo skin optical clearing efficacy quantification of clinically compatible agents using line-field confocal optical coherence tomography

Estimation of Scattering Properties Modifications Caused by In Vivo Human Skin Optical Clearing Using Line‐Field Confocal Optical Coherence Tomography

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