Optical clearing for multiscale biological tissues

Yu, Tingting; Qi, Yisong; Gong, Hui; Luo, Qingming; Zhu, Dan

doi:10.1002/jbio.201700187

Cited by 71 publications

(56 citation statements)

References 124 publications

(245 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to increase the probing depth, light‐focusing ability, spatial resolution of optical systems and image contrast, the optical clearing (OC) technique, allowing for control of tissue optical properties, has been proposed. Fundamentals and advances of OC technologies for in vitro and in vivo applications were recently reviewed in a number of publications . The influence of different OC agents (OCAs), for example, glycerol , glucose , dimethyl sulfoxide (DMSO) , scale , Sca/eS , PEG 400 and thiazone , was studied by various optical techniques such as OCT , MPT , RS , CRM and so on .…”

Section: Introductionmentioning

confidence: 99%

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

Confocal Raman microscopy has been used to measure depth‐dependent profiles of porcine skin ex vivo in the high wavenumber region after application of molecular optical clearing agents (OCAs). Glycerol (70%) and iohexol (100% Omnipaque [300]) water solutions were used as OCAs and topically applied to porcine ear skin for 30 and 60 minutes. Using Gaussian function–based deconvolution, the changes of hydrogen bound water molecule types have been microscopically analyzed down to the depth of 200 μm. Results show that both OCAs induced skin dehydration (reduction of total water), which is 51.3% for glycerol (60 minutes), 33.1% for glycerol (30 minutes), 8.3% for Omnipaque (60 minutes) and 4.4% for Omnipaque (30 minutes), on average for the 40 to 200 μm depths. Among the water types in the skin, the following reduction was observed in concentration of weakly bound (51.1%, 33.2%, 7.5% and 4.6%), strongly bound (50.4%, 33.0%, 7.9% and 3.4%), tightly bound (63.6%, 42.3%, 26.1% and 12.9%) and unbound (55.4%, 28.7%, 10.1% and 5.9%) water types on average for the 40 to 200 μm depths, post application of glycerol (60 minutes), glycerol (30 minutes), Omnipaque (60 minutes) and Omnipaque (30 minutes), respectively. As most concentrated in the skin, weakly and strongly bound water types are preferentially involved in the OCA‐induced water flux in the skin, and thus, are responsible for optical clearing efficiency.

show abstract

Section: Introductionmentioning

confidence: 99%

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Sdobnov

Darvin

Schleusener

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

show abstract

“…preserves the lipidic content of each sample, thus allowing the imaging of these structures using lipophilic tracer dyes (e.g., DiI and FM 1-43FX; Yu et al, 2018). preserves the lipidic content of each sample, thus allowing the imaging of these structures using lipophilic tracer dyes (e.g., DiI and FM 1-43FX; Yu et al, 2018).…”

Section: Clsmmentioning

confidence: 99%

“…Currently, the combination of the strengths of different methods has been supporting the development of procedures that allow to obtain better clearing and imaging without affecting the structure and the endogenous/exogenous fluorescence of the sample, as discussed by Yu et al (2018). Nevertheless, it is expected that future research will further result in increased clearing efficacies and improved imaging of thick samples.…”

Section: Outlook and Future Directionsmentioning

confidence: 99%

“…The clearing methods were originally developed for processing large samples obtained from animals (e.g., bone, brain, embryo, heart, intestine, kidney, lung, muscle, pancreas, spinal cord, spleen, testis, among others), as already reviewed in detail in (Feuchtinger et al, 2016;Genina et al, 2010;Lee, Kim, & Sun, 2016;Richardson & Lichtman, 2015;Seo et al, 2016;Silvestri, Costantini, Sacconi, & Pavone, 2016;Susaki & Ueda, 2016;Tainaka, Kuno, Kubota, Murakami, & Ueda, 2016;Tuchin, 2015;Yu, Qi, Gong, Luo, & Zhu, 2018;Zhu, Larin, Luo, & Tuchin, 2013 (1.4-1.5; Figure 2; Ariel, 2017;Seo et al, 2016). Due to the osmotic pressure, the water content in the sample (that has low RI ≈ 1.33) will be passively replaced by the clearing solution.…”

Section: Light Scattering and Tactics Of Optical Clearingmentioning

confidence: 99%

See 1 more Smart Citation

Optical clearing methods: An overview of the techniques used for the imaging of 3D spheroids

Costa

Silva

Moreira

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

Spheroids have emerged as in vitro models that reproduce in a great extent the architectural microenvironment found in human tissues. However, the imaging of 3D cell cultures is highly challenging due to its high thickness, which results in a lightscattering phenomenon that limits light penetration. Therefore, several optical clearing methods, widely used in the imaging of animal tissues, have been recently explored to render spheroids with enhanced transparency. These methods are aimed to homogenize the microtissue refractive index (RI) and can be grouped into four different categories, namely (a) simple immersion in an aqueous solution with high RI;(b) delipidation and dehydration followed by RI matching; (c) delipidation and hyperhydration followed by RI matching; and (d) hydrogel embedding followed by delipidation and RI matching. In this review, the main optical clearing methods, their mechanism of action, advantages, and disadvantages are described. Furthermore, the practical examples of the optical clearing methods application for the imaging of 3D spheroids are highlighted. K E Y W O R D Simaging, light scattering, optical clearing, optical microscopy, spheroids.

show abstract

“…Furthermore, new optical clearing techniques for large mammalian systems, such as Clarity, Scale, CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis), 3DISCO (3-dimensional imaging of solvent-cleared organs), SeeDB (see deep brain), ISDoT (in situ decellularization of tissues) or PACT (photoacoustic computed tomography), have improved the imaging resolution at depth by removing the cellular components of tissues that cause its opacity (Chung and Deisseroth, 2013;Fischer et al, 2017;Mayorca-Guiliani et al, 2017;Richardson and Lichtman, 2015;Tainaka et al, 2014;Tomer et al, 2014;Yu et al, 2017). Future combination of intravital studies with post-intravital optical clearing and subsequent high-resolution imaging, similar to CLEM, may provide new insight into disease aetiology and treatment.…”

Section: Future Applications and Combined Imaging Modesmentioning

confidence: 99%

Molecular mobility and activity in an intravital imaging setting – implications for cancer progression and targeting

Nobis

Warren

Lucas

et al. 2018

Journal of Cell Science

View full text Add to dashboard Cite

Molecular mobility, localisation and spatiotemporal activity are at the core of cell biological processes and deregulation of these dynamic events can underpin disease development and progression. Recent advances in intravital imaging techniques in mice are providing new avenues to study real-time molecular behaviour in intact tissues within a live organism and to gain exciting insights into the intricate regulation of live cell biology at the microscale level. The monitoring of fluorescently labelled proteins and agents can be combined with autofluorescent properties of the microenvironment to provide a comprehensive snapshot of in vivo cell biology. In this Review, we summarise recent intravital microscopy approaches in mice, in processes ranging from normal development and homeostasis to disease progression and treatment in cancer, where we emphasise the utility of intravital imaging to observe dynamic and transient events in vivo. We also highlight the recent integration of advanced subcellular imaging techniques into the intravital imaging pipeline, which can provide in-depth biological information beyond the singlecell level. We conclude with an outlook of ongoing developments in intravital microscopy towards imaging in humans, as well as provide an overview of the challenges the intravital imaging community currently faces and outline potential ways for overcoming these hurdles.

show abstract

Optical clearing for multiscale biological tissues

Cited by 71 publications

References 124 publications

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Hydrogen bound water profiles in the skin influenced by optical clearing molecular agents—Quantitative analysis using confocal Raman microscopy

Optical clearing methods: An overview of the techniques used for the imaging of 3D spheroids

Molecular mobility and activity in an intravital imaging setting – implications for cancer progression and targeting

Contact Info

Product

Resources

About