Optical clearing of in vivo human skin: Implications for light‐based diagnostic imaging and therapeutics

Khan, M. Ali; Choi, Bernard; Chess, Samuel; Kelly, Kristen M.; McCullough, Jerry L.; Nelson, J. Stuart

doi:10.1002/lsm.20014

Cited by 103 publications

(94 citation statements)

References 6 publications

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“…However, the similar improvement in blood vessels imaging using OCT could be not found at application of glycerol as an OCA [64][65][66]. The main reasons may be from the two aspects: firstly, thiazone, as a chemical penetration agent, was used in our work, could significantly enhance the skin optical clearing efficacy [39][40][41], as well as the penetration of glycerol into skin sample is relatively limited [29,67,68]. In addition, the thickness of mice skin in our study is thinner than human skin.…”

Section: Discussionmentioning

confidence: 82%

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Shi¹,

Chen²,

Tuchin³

et al. 2015

Biomed. Opt. Express

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Laser speckle contrast imaging (LSCI) shows a great potential for monitoring blood flow, but the spatial resolution suffers from the scattering of tissue. Here, we demonstrate the capability of a combination method of LSCI and skin optical clearing to describe in detail the dynamic response of cutaneous vasculature to vasoactive noradrenaline injection. Moreover, the superior resolution, contrast and sensitivity make it possible to rebuild arteries-veins separation and quantitatively assess the blood flow dynamical changes in terms of flow velocity and vascular diameter at single artery or vein level.

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Section: Discussionmentioning

confidence: 82%

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Shi¹,

Chen²,

Tuchin³

et al. 2015

Biomed. Opt. Express

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“…New original results obtained using the combination of optical clearing with the known optical visualisation methods, such as laser speckle-contrast imaging [34, 71,75,77,79], OCT [20,29,35,[59][60][61]65,69,80,82], microscopic imaging [23,24,83,84], ultra-microscopy [85][86][87], etc., demonstrated high potentiality of their mutual use not only for getting high-resolution structure and functional tissue images in vitro [72,73,[83][84][85][86][87], but also for optical imaging and diagnostics in vivo [25,34,45,50,59,61,65,68,70,71,[75][76][77][79][80][81]. Table 1 shows the increase of the light penetration depth, caused by clearing agents in some diagnostic methods.…”

Section: Immersion Clearingmentioning

confidence: 99%

“…These factors can essentially modify the kinetic characteristics and the magnitude of the clearing effect [25,34,45,59,61,68,70,76,77,79,80,82,97,98,108,113,118,119]. In living tissues the refractive index is a function of the physiological or pathological condition of the tissue.…”

Section: Immersion Clearingmentioning

confidence: 99%

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Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

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“…Our group has recently reported the use of topically applied hyperosmotic, optical clearing agents composed of a pre-polymer mixture of polypropylene glycol and polyethylene glycol, PPG:PEG that can penetrate intact human skin [5] and temporarily alter local optical properties in vivo. Epidermal and papillary dermal scattering events are reduced significantly and more light penetrates deeper into the skin.…”

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confidence: 99%

Can topically applied optical clearing agents increase the epidermal damage threshold and enhance therapeutic efficacy?

et al. 2004

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Optical clearing of in vivo human skin: Implications for light‐based diagnostic imaging and therapeutics

Cited by 103 publications

References 6 publications

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Accessing to arteriovenous blood flow dynamics response using combined laser speckle contrast imaging and skin optical clearing

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Can topically applied optical clearing agents increase the epidermal damage threshold and enhance therapeutic efficacy?

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