Optical coherence tomography angiography for noninvasive evaluation of angiogenesis in a limb ischemia mouse model

Wang, Liwei; Chen, Zuoguan; Li, Yongjun; Yang, Jing; Li, Yuejie

doi:10.1038/s41598-019-42520-3

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…The animal model of hindlimb ischemia was established according to our previously described methods [ 26 ]. Briefly, animals were anesthetized by intraperitoneal administration of chloral hydrate (400 mg/kg), and the hair on their limbs was completely removed with depilatory cream and wiped with ethanol.…”

Section: Methodsmentioning

confidence: 99%

ROS-responsive capsules engineered from EGCG-Zinc networks improve therapeutic angiogenesis in mouse limb ischemia

Chen

Duan

Diao

et al. 2021

Bioactive Materials

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The successful treatment of limb ischemia requires that promote angiogenesis along with microenvironment improvement. Zinc ions have been reported to stimulate angiogenesis, but application was limited to the toxicity concerns. We hypothesized that zinc based metal-EGCG capsule (EGCG/Zn Ps) can achieve sustained release Zn 2+ resulting in reduced toxicity and improve angiogenesis as well as the improvement of microenvironment by ROS scavenging of EGCG. The surface morphology, zeta potential, infrared absorbance peaks and zinc ion release profile of the EGCG/Zn Ps were measured. In vitro, EGCG/Zn showed significantly antioxidant, anti-inflammatory and induced cell migration effect. In addition, EGCG/Zn Ps enabled the sustained release of zinc ions, which reduced cytotoxicity and enhanced the secretion of vascular endothelial growth factor (VEGF) in vitro and in vivo. In mouse models of limb ischemia, EGCG/Zn Ps promoted angiogenesis and cell proliferation in ischemic tissues. Moreover, EGCG/Zn Ps group exhibited the most significant recovery of limb ischemic score, limb temperature and blood flow than other groups. In conclusion, EGCG/Zn Ps is a safe and promising approach to combine the merit of Zn 2+ and EGCG, thus enabling the direct application to limb ischemia.

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Section: Methodsmentioning

confidence: 99%

ROS-responsive capsules engineered from EGCG-Zinc networks improve therapeutic angiogenesis in mouse limb ischemia

Chen

Duan

Diao

et al. 2021

Bioactive Materials

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“…In the ISC algorithm, the changes in the OCT signal intensity between subsequent B-scans caused by the moving particles are calculated. 12,29 To calculate the OCTA signal, the amplitude of the OCT signals from each of the 4 B-scans was used to calculate the speckle contrast image k s…”

Section: Isc Algorithmmentioning

confidence: 99%

Comparative study of optical coherence tomography angiography algorithms for rodent retinal imaging

Dadkhah

Paudel

Jiao

2021

Exp Biol Med (Maywood)

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Optical coherence tomography angiography (OCTA) is a functional extension of optical coherence tomography for non-invasive in vivo three-dimensional imaging of the microvasculature of biological tissues. Several algorithms have been developed to construct OCTA images from the measured optical coherence tomography signals. In this study, we compared the performance of three OCTA algorithms that are based on the variance of phase, amplitude, and the complex representations of the optical coherence tomography signals for rodent retinal imaging, namely the phase variance, improved speckle contrast, and optical microangiography. The performance of the different algorithms was evaluated by comparing the quality of the OCTA images regarding how well the vasculature network can be resolved. Quantities that are widely used in ophthalmic studies including blood vessel density, vessel diameter index, vessel perimeter index, vessel complexity index were also compared. Results showed that both the improved speckle contrast and optical microangiography algorithms are more robust than phase variance, and they can reveal similar vasculature features while there are statistical differences in the calculated quantities.

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“…In principle, OCT has a submillimeter to micrometer resolution, which might suffice to visualize networks. Additionally, OCT allows repetitive measurements of 3D cultures over time so as to follow the formation of networks [ 20 ]. Thus, fluorescence inverted microscopy, stacked confocal laser scanning microscopy, and OCT allow for repeated measurements, while destructive methods that rely on embedding, sectioning, and staining do not.…”

Section: Introductionmentioning

confidence: 99%

From Macro to Micro: Comparison of Imaging Techniques to Detect Vascular Network Formation in Left Ventricle Decellularized Extracellular Matrix Hydrogels

Zhang

Getova

Martínez-García

et al. 2022

Gels

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Background: Angiogenesis is a crucial process in physiological maintenance and tissue regeneration. To understand the contribution of angiogenesis, it is essential to replicate this process in an environment that reproduces the biochemical and physical properties which are largely governed by the extracellular matrix (ECM). We investigated vascularization in cardiac left ventricular ECM hydrogels to mimic post-myocardial repair. We set out to assess and compare different destructive and non-destructive methods, optical as well as non-optical, to visualize angiogenesis and associated matrix remodeling in myocardial ECM hydrogels. Methods: A total of 100,000, 300,000, and 600,000 Human Pulmonary Microvascular Endothelial Cells (HPMEC) were seeded in left ventricular cardiac ECM hydrogel in 48-well plates. After 1, 7, and 14 days of culture, the HPMEC were imaged by inverted fluorescence microscopy and 3D confocal laser scanning microscopy (Zeiss Cell Discoverer 7). In addition, cell-seeded ECM hydrogels were scanned by optical coherence tomography (OCT). Fixed and paraffin-embedded gels were thin-sectioned and assessed for ECM components via H&E, picrosirius red histochemical staining, and immunostaining for collagen type I. ImageJ-based densitometry was used to quantify vascular-like networks and GraphPad was used for statistical analyses. Results: Qualitative analyses were realized through fluoromicrographs obtained by the confocal laser scanning microscope which allowed us to visualize the extensive vascular-like networks that readily appeared at all seeding densities. Quantification of networks was only possible using fluoromicrographs from inverted microscopy. These showed that, after three days, the number of master junctions was seeding density-dependent. The resolution of optical coherence tomography was too low to distinguish between signals caused by the ECM and cells or networks, yet it did show that gels, irrespective of cells, were heterogeneous. Interestingly, (immuno)histochemistry could clearly distinguish between the cast cardiac-derived matrix and newly deposited ECM in the hydrogels. The H&E staining corroborated the presence of vascular-like network structures, albeit that sectioning inevitably led to the loss of 3D structure. Conclusions: Except for OCT, all methods had complementary merit and generated qualitative and quantitative data that allowed us to understand vascular network formation in organ-derived ECM hydrogels.

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Optical coherence tomography angiography for noninvasive evaluation of angiogenesis in a limb ischemia mouse model

Cited by 6 publications

References 31 publications

ROS-responsive capsules engineered from EGCG-Zinc networks improve therapeutic angiogenesis in mouse limb ischemia

ROS-responsive capsules engineered from EGCG-Zinc networks improve therapeutic angiogenesis in mouse limb ischemia

Comparative study of optical coherence tomography angiography algorithms for rodent retinal imaging

From Macro to Micro: Comparison of Imaging Techniques to Detect Vascular Network Formation in Left Ventricle Decellularized Extracellular Matrix Hydrogels

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