Optical Coherence Tomography Is a Promising Tool for Zebrafish-Based Research—A Review

Lichtenegger, Antonia; Baumann, Bernhard; Yasuno, Yoshiaki

doi:10.3390/bioengineering10010005

Cited by 15 publications

(6 citation statements)

References 127 publications

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“…With this PIC-based system, we can acquire in vivo tomograms that provide relevant morphological information in human fingertips and zebrafish larvae. Zebrafish are widely used as a model for human pathogenesis [36], including heart failure models [37] or cancer models [38][39][40]. Figure 4a displays the 3D-tomogram of a whole zebrafish larva at an age of 4.5 days post fertilisation (video in the extended data).…”

Section: In Vivo Oct Measurementsmentioning

confidence: 99%

CMOS Optoelectronic Spectrometer based on Photonic Integrated Circuits for In Vivo 3D Optical Coherence Tomography

Drexler,

Agneter,

Müllner

et al. 2024

Preprint

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Photonics integrated circuits (PICs) represent a promising technology for the much-needed medical devices of today. Their primary advantage lies in their ability to integrate multiple functions onto a single chip, thereby reducing the complexity, size, maintenance requirements, and costs. When applied to optical coherence tomography (OCT), the leading tool for state-of-the-art ophthalmic diagnosis, PICs have the potential to increase accessibility, especially in scenarios, where size, weight, or costs are limiting factors. In this paper, we introduce an unprecedented fully integrated CMOS-compatible spectrometer, an optoelectronic system based on PIC for spectral domain OCT. To achieve this, we co-integrated a 512-channel arrayed waveguide grating with electronics. We successfully addressed the challenge of establishing a connection with minimal losses between the optical components and photodiodes co-integrated on a chip achieving a transmission efficiency of 70%. With this fully integrated PIC-based spectrometer interfaced to a spectral domain OCT system, we achieved a sensitivity of 92dB at an imaging speed of 55kHz, with a 6dB signal roll-off occurring at 2mm. We applied this innovative technology successfully to obtain 3D in vivo tomograms of zebrafish larvae and human skin. This ground-breaking fully integrated spectrometer represents a significant step towards a miniaturised, cost-effective, and maintenance-free OCT system.

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Section: In Vivo Oct Measurementsmentioning

confidence: 99%

CMOS Optoelectronic Spectrometer based on Photonic Integrated Circuits for In Vivo 3D Optical Coherence Tomography

Drexler,

Agneter,

Müllner

et al. 2024

Preprint

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“…OCT is well-suited for live imaging of small animal embryos because of its micrometer-scale resolution, noninvasive nature, imaging depth of a few millimeters in scattering tissues, and relatively high speed. 10,11,13 . However, OCT relies on backscattered light from intrinsic fluctuations in tissue optical properties.…”

Section: Introductionmentioning

confidence: 99%

Imaging zebrafish embryonic development with multimodal optical coherence tomography and light-sheet fluorescence microscopy

Karim,

Sun,

Ruiz

et al. 2024

Multimodal Biomedical Imaging XIX

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Zebrafish are a widely used developmental model because of their transparent embryos and external development. These distinctive characteristics provide valuable insights into embryonic development. Optical coherence tomography (OCT) offers label-free structural imaging and has emerged as a preferred tool for embryonic imaging. On the other hand, light sheet fluorescence microscopy (LSFM) enables time-lapse molecular imaging of multi-hour to multi-day developmental processes due to its low phototoxicity and photobleaching compared to traditional confocal fluorescence microscopy. We developed a multimodal imaging system to obtain concurrent structural and molecular information by combining OCT and LSFM for embryonic imaging. A Michelson-type swept-source OCT system with a central wavelength of 1050 nm, the bandwidth of 100 nm, and sweep rate of 100 kHz captured the structural information with a lateral resolution of ~15 µm and an axial resolution of ~7 µm. The LSFM system captured the molecular information with a transverse resolution of ~2.1 µm and an axial resolution of ~13 µm. The optically co-aligned OCT and LSFM beams were scanned through the same scan head for trivial co-registration of the multimodal images. We imaged 1-5 µm green fluorescence microbeads to show the capability of this system. We then conducted imaging of zebrafish vasculature development with a transgenic line, Tg(kdrl:EGFP), where the erythroblasts express GFP. The results show that the multimodal system enables us to provide co-registered zebrafish structural and functional imaging.

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“…OCM, which uses a higher numerical aperture (NA), can achieve high lateral resolution but usually is limited in penetration depth. OCT/OCM has emerged as a valuable imaging modality for living tissues and model organisms 26 – 28 and more recently for organoids and spheroids 29 – 31 , providing high-resolution information on the internal structural organization inside the organoids non-invasively and label-free.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-induced reorientation for multi-angle optical coherence tomography

Kvåle Løvmo,

Deng,

Moser

et al. 2024

Nat Commun

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Organoid and spheroid technology provide valuable insights into developmental biology and oncology. Optical coherence tomography (OCT) is a label-free technique that has emerged as an excellent tool for monitoring the structure and function of these samples. However, mature organoids are often too opaque for OCT. Access to multi-angle views is highly desirable to overcome this limitation, preferably with non-contact sample handling. To fulfil these requirements, we present an ultrasound-induced reorientation method for multi-angle-OCT, which employs a 3D-printed acoustic trap inserted into an OCT imaging system, to levitate and reorient zebrafish larvae and tumor spheroids in a controlled and reproducible manner. A model-based algorithm was developed for the physically consistent fusion of multi-angle data from a priori unknown angles. We demonstrate enhanced penetration depth in the joint 3D-recovery of reflectivity, attenuation, refractive index, and position registration for zebrafish larvae, creating an enabling tool for future applications in volumetric imaging.

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Optical Coherence Tomography Is a Promising Tool for Zebrafish-Based Research—A Review

Cited by 15 publications

References 127 publications

CMOS Optoelectronic Spectrometer based on Photonic Integrated Circuits for In Vivo 3D Optical Coherence Tomography

CMOS Optoelectronic Spectrometer based on Photonic Integrated Circuits for In Vivo 3D Optical Coherence Tomography

Imaging zebrafish embryonic development with multimodal optical coherence tomography and light-sheet fluorescence microscopy

Ultrasound-induced reorientation for multi-angle optical coherence tomography

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