Optical cytosensors for the detection of circulating tumour cells

Vajhadin, Fereshteh; Mazloum, Mohammad; Sanati, Alireza; Haghniaz, Reyhaneh; Travaš-Sejdić, Jadranka

doi:10.1039/d1tb02370e

Cited by 18 publications

(5 citation statements)

References 96 publications

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“…Because CTCs are whole packages containing proteins and nucleic acids, avoiding cell rupture and gently retrieving of viable CTCs for downstream analysis are also important. In addition, the heterogeneity of CTCs and a lack of their specific surface marker should be considered in the development of sensing technologies [ 219 ]. Notably, CTCs are the largest biomarker in liquid biopsy.…”

Section: Optical Nanomaterial-based Detection Of Lb Biomarkersmentioning

confidence: 99%

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Kim,

Rho,

Park

et al. 2024

J Hematol Oncol

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Liquid biopsy, which is a minimally invasive procedure as an alternative to tissue biopsy, has been introduced as a new diagnostic/prognostic measure. By screening disease-related markers from the blood or other biofluids, it promises early diagnosis, timely prognostication, and effective treatment of the diseases. However, there will be a long way until its realization due to its conceptual and practical challenges. The biomarkers detected by liquid biopsy, such as circulating tumor cell (CTC) and circulating tumor DNA (ctDNA), are extraordinarily rare and often obscured by an abundance of normal cellular components, necessitating ultra-sensitive and accurate detection methods for the advancement of liquid biopsy techniques. Optical biosensors based on nanomaterials open an important opportunity in liquid biopsy because of their enhanced sensing performance with simple and practical properties. In this review article, we summarized recent innovations in optical nanomaterials to demonstrate the sensitive detection of protein, peptide, ctDNA, miRNA, exosome, and CTCs. Each study prepares the optical nanomaterials with a tailored design to enhance the sensing performance and to meet the requirements of each biomarker. The unique optical characteristics of metallic nanoparticles (NPs), quantum dots, upconversion NPs, silica NPs, polymeric NPs, and carbon nanomaterials are exploited for sensitive detection mechanisms. These recent advances in liquid biopsy using optical nanomaterials give us an opportunity to overcome challenging issues and provide a resource for understanding the unknown characteristics of the biomarkers as well as the mechanism of the disease. Graphical abstract

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Section: Optical Nanomaterial-based Detection Of Lb Biomarkersmentioning

confidence: 99%

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Kim,

Rho,

Park

et al. 2024

J Hematol Oncol

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“…Fluorescence-based assays for the analysis of tumor cell biomarkers are more responsive than electrochemical assays 74 . In addition to the detection of extracellular tumor markers in body fluids using fluorescent biosensors, the in situ detection of intracellular tumor biomarkers in living cells by combining fluorescence analysis with nanodevices is also gaining attention 75 .…”

Section: Fluorescence Sensingmentioning

confidence: 99%

Current research status of tumor cell biomarker detection

Jiang,

Lin,

Chen

et al. 2023

Microsyst Nanoeng

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With the annual increases in the morbidity and mortality rates of tumors, the use of biomarkers for early diagnosis and real-time monitoring of tumor cells is of great importance. Biomarkers used for tumor cell detection in body fluids include circulating tumor cells, nucleic acids, protein markers, and extracellular vesicles. Among them, circulating tumor cells, circulating tumor DNA, and exosomes have high potential for the prediction, diagnosis, and prognosis of tumor diseases due to the large amount of valuable information on tumor characteristics and evolution; in addition, in situ monitoring of telomerase and miRNA in living cells has been the topic of extensive research to understand tumor development in real time. Various techniques, such as enzyme-linked immunosorbent assays, immunoblotting, and mass spectrometry, have been widely used for the detection of these markers. Among them, the detection of tumor cell markers in body fluids based on electrochemical biosensors and fluorescence signal analysis is highly preferred because of its high sensitivity, rapid detection and portable operation. Herein, we summarize recent research progress in the detection of tumor cell biomarkers in body fluids using electrochemical and fluorescence biosensors, outline the current research status of in situ fluorescence monitoring and the analysis of tumor markers in living cells, and discuss the technical challenges for their practical clinical application to provide a reference for the development of new tumor marker detection methods.

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“…1,2 Fluorescence detection can not only circumvent the danger of handling radioactive materials but also detect multiple signals and track individual molecules, and describe the physiological processes of many diseases, which has found extensive utilization within the biomedical domain. 3,4 Up until now, a variety of fluorescent sensing system, including fluorescent proteins, 5,6 quantum dots, 7 and organic dyes, 8 have been developed and widely applied as biosensors. 9,10 Recently, there has been a growing attention devoted to the study of polymerbased fluorescent sensing system or probes.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Fluorescence detection analysis has become a cornerstone of bioanalytical and diagnostic applications, praised for its simplicity, high sensitivity, and anti-interference properties. , Fluorescence detection can not only circumvent the danger of handling radioactive materials but also detect multiple signals and track individual molecules, and describe the physiological processes of many diseases, which has found extensive utilization within the biomedical domain. , Up until now, a variety of fluorescent sensing system, including fluorescent proteins, , quantum dots, and organic dyes, have been developed and widely applied as biosensors. , Recently, there has been a growing attention devoted to the study of polymer-based fluorescent sensing system or probes. Polymer fluorescent probes not only maintain the properties of small monomers but also exhibit unique properties due to the polymeric synergistic effect .…”

Section: Introductionmentioning

confidence: 99%

Preparation of a Fluorescent Polymer Sensing System with an Oxidation-Electric Dual-Mode Response Based on the Supramolecular Host–Guest Interaction

Shen,

Lu,

Ren

et al. 2024

ACS Appl. Polym. Mater.

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Nowadays, stimuli-responsive macromolecular self-assembly systems have significant potential in biomedicine and molecular regulation. Their combination with fluorescent molecules is particularly important in the design of a sensing system. In this work, we designed a stimuli-responsive fluorescent sensing system (PF-b -PFMMA-b-PPEGMA@CD) based on the self-assembly of cyclodextrins (CD) with ferrocene (Fc), which consisted of a water-soluble conjugated polymer composed of a polyfluorene backbone with fluorescent properties, a segment providing the self-assembled guest groups, and a segment improving water solubility, together with the modified cyclodextrin. The sensing system exhibited good sensitivity to oxidizing substances and electrical stimulation. Under self-assembly, the fluorescence of the aggregates was quenched. Due to the inclusion of ferrocene, Fc became hydrophilic and is oxidized into charged Fc+, leading to disassembly and fluorescence recovery, which can be used to construct the fluorescent sensing system for detecting biomolecular substrates. Furthermore, based on its high sensitivity to oxidizing molecules and electrical stimulation, we evaluated its application potential as a drug delivery system and believe that this system has significance for advancing the development of fluorescent polymers in the field of medicine.

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Optical cytosensors for the detection of circulating tumour cells

Abstract: Blood analysis is an established approach to monitor various diseases, ranging from heart defects and diabetes to cancer. Among various tumor markers in the blood, circulating tumor cells (CTCs) have...

Cited by 18 publications

References 96 publications

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Optical nanomaterial-based detection of biomarkers in liquid biopsy

Current research status of tumor cell biomarker detection

Preparation of a Fluorescent Polymer Sensing System with an Oxidation-Electric Dual-Mode Response Based on the Supramolecular Host–Guest Interaction

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