Optical detection of antibody using silica–silver core–shell particles

Kalele, Suchita; Ashtaputre, Shriwas; Hebalkar, Neha; Gosavi, Suresh; Deobagkar, Dileep N.; Kulkarni, Sulabha K.

doi:10.1016/j.cplett.2005.01.064

Cited by 72 publications

(40 citation statements)

References 19 publications

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“…As an alternative to the drastic optical changes due to nanoparticle aggregation, one can monitor the more subtle effect of the binding of a target molecule to the nanoparticle surface [62][63][64][65][66]. The presence of the target molecule alters the local dielectric environment of the nanoparticle, which shifts the LSPR peak wavelength.…”

Section: Biosensing Applicationsmentioning

confidence: 99%

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

2006

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The strong optical absorption and scattering of noble metal nanoparticles is due to an effect called localized surface plasmon resonance, which enables the development of novel biomedical applications. The resonant extinction, which can be tuned to the near-infrared, allows the nanoparticles to act as molecular contrast agents in a spectral region where tissue is relatively transparent. The localized heating due to resonant absorption, also tunable into the near-infared, enables new thermal ablation therapies and drug delivery mechanisms. The sensitivity of these resonances to their environment leads to simple affinity sensors for the detection of low-level molecular analytes. Coupled with their general lack of toxicity, these applications suggest that noble metal nanoparticles are a highly promising class of nanomaterials for new biomedical applications.

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Section: Biosensing Applicationsmentioning

confidence: 99%

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

2006

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“…Hollow spheres represent a fascinating class of materials, which has attracted an immense interest of the researchers because of their potential applications in bioanalytical area catalysis, magnetics, ceramics, pigments and biosensor [12][13][14][15]. Research has demonstrated a clear ZnO size and shape dependence of its electromagnetic, optical, and catalytic properties [16].…”

Section: Introductionmentioning

confidence: 99%

Direct electron transfer and electrochemical study of hemoglobin immobilized in ZnO hollow spheres

Liu

Zhang

2011

Bioprocess Biosyst Eng

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ZnO hollow spheres were firstly prepared. A new type of amperometric hydrogen peroxide biosensor was fabricated by entrapping Hemoglobin (Hb) through the ZnO hollow spheres (ZHS) nanoparticles. The composition morphology and size were studied by transmission electron microscopy. The surface topography of the prepared films was imaged by atomic force microscope (AFM). Several techniques, including UV-vis absorption spectroscopy, cyclic voltammetry, chronoamperometry were employed to characterize the performance of the biosensor. The results indicated that the ZHS nanoparticles had enhanced the performance of the hydrogen peroxide sensors. The electrochemical parameters of Hb in the ZHS were calculated by the results of the electron-transfer coefficient (α) and the apparent heterogeneous electron-transfer rate constant K (s) as 0.5 and 3.1 s(-1), respectively. The resulting biosensors showed a wide linear range from 2.1 × 10(-6) to 5.18 × 10(-3) M, with a low detection limit of 7.0 × 10(-7) M (S/N = 3) under optimized experimental conditions. The results demonstrated that the ZHS matrix may improve the protein loading with the retention of bioactivity and greatly promote the direct electron transfer, which can be attributed to its unique morphology, high specific surface area, and biocompatibility. The biosensor obtained from this study possesses high sensitivity, good reproducibility, and long-term stability.

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“…1 AgNPs have been exploited in designing silica-silver core-shell nanoparticles and utilized for rapid detection of microbes, proteins, and antibodies. 2,3 AgNPs have also been used in bone cements in artificial joint replacements, since their presence drastically reduced the wear and tear of the polymer. 4,5 Although AgNPs have promising role in biomedical applications, human toxicity and environmental hazards posed by them cannot be neglected.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

Kulkarni¹,

Shaiwale²,

Deobagkar³

et al. 2015

IJN

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There has been rapid progress in exploring microorganisms for green synthesis of nanoparticles since microbes show extraordinary diversity in terms of species richness and niche localization. Microorganisms are easy to culture using relatively inexpensive and simple nutrients under varied conditions of temperature, pressure, pH, etc. In this work, Deinococcus radiodurans that possesses the ability to withstand extremely high radiation and desiccation stress has been employed for the synthesis of silver nanoparticles (AgNPs). D. radiodurans was able to accumulate AgNPs in medium under various conditions, and process optimization was carried out with respect to time, temperature, pH, and concentration of silver salt. AgNPs were characterized using UV/vis spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The microbially synthesized AgNPs exhibited good antimicrobial activity against both Gram-negative and Gram-positive organisms and anti-biofouling activity. Their ability to inhibit growth and proliferation of cancer cell line was also examined, and it could be seen that AgNPs synthesized using D. radiodurans exhibited excellent anticancer activity.

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Optical detection of antibody using silica–silver core–shell particles

Cited by 72 publications

References 19 publications

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

Biomedical Applications of Plasmon Resonant Metal Nanoparticles

Direct electron transfer and electrochemical study of hemoglobin immobilized in ZnO hollow spheres

Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

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