Optical Determination of Glucose with Glucose Oxidase Immobilized in PVC together with Fluorescent Oxazol‐5‐One Derivatives

Abstract

Fully reversible biosensors for glucose monitoring based on solid polyvinylchloride (PVC) films where the enzyme glucose oxidase (GOx) was incorporated together with 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadec yl)benzylidene]-5-oxazolone (CPO-1), 2-(3,5-dinitrophenyl)-4-[4-(1,4,7,10-tetra oxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone (CPO-2), 2-(4-nitrophenyl) -4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone (CPO-3) and 2-(4-tolyl)-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone (CPO-4) derivatives have been developed. The limit of detection (LOD) values for glucose were found to be 1.47 Â 10 25 M, 2.01 Â 10 25 M, 0.89 Â 10 25 M, and 0.12 Â 10 25 M for CPO-1, CPO-2, CPO-3, and CPO-4, respectively (n ¼ 7). Sensor films were found to have excellent photostability.

Simultaneous and Sequential Synthesis of Polyaniline-g-poly(ethylene glycol) by Combination of Oxidative Polymerization and CuAAC Click Chemistry: A Water-Soluble Instant Response Glucose Biosensor Material

“…In addition, glucose monitoring is important for both chronic diabetes as well as nondiabetic acute care patients as large variations in blood glucose level may cause complications resulting from surgery or acute illness. , Thus, intensive research activity has focused on the design of various sensing materials and methodologies for accurate, comparatively noninvasive and continuous glucose detection . Various analytical methods utilizing glucose oxidase (GOx) have been developed. − The usually applied methodology involves their immobilization into various stable polymer matrixes …”

Synthesis of a Biologically Active Oxazol-5-(4H)-one via an Erlenmeyer–Plöchl Reaction

“…The exocyclic double bond in position 4 (Figure b) of the oxazolone ring forms a highly conjugated system with strong absorbance and tunable fluorescence that enables their application in photochemistry and materials sciences . They have been reported as sensors (pH, Fe 3+ , glucose, among others), as photoswitches, and also as having nonlinear optical properties including two-photon absorption. , …”

Fluorescence Sensing of Glucose Using Glucose Oxidase Modified by PVA-Pyrene Prepared via “Click” Chemistry

“…In addition to the need for glucose monitoring in the case of patients with chronic diabetes, there is a critical need for monitoring glucose levels in nondiabetic acute care patients because large variations in blood glucose level have been observed also in nondiabetic patients as a common consequence of surgery or acute illness, mostly for those suffering from stress-induced hyperglycemia. , The crucial demand for continuous, accurate, and relatively noninvasive glucose sensing methods has motivated the design of various sensing materials as well as methodologies . Various analytical methods such as amperometry, potentiometry, spectrophotometry, or fluorometry are used for the enzymatic measurements of glucose concentration. − Among these methods, those utilizing the glucose oxidase (GOx) enzyme are the most widespread, especially in the fabrication of new glucose sensors . The sensing mechanism and the enzyme immobilization method are crucial aspects of the biosensor design.…”

“…11 Various analytical methods such as amperometry, potentiometry, spectrophotometry, or fluorometry are used for the enzymatic measurements of glucose concentration. [13][14][15][16] Among these methods, those utilizing the glucose oxidase (GOx) enzyme are the most widespread, especially in the fabrication of new glucose sensors. 11 The sensing mechanism and the enzyme immobilization method are crucial aspects of the biosensor design.…”