Optical device and methodology for optical sensing of hemolysis in hypotonic media

Márquez-Islas, R.; Pérez-Pacheco, Argelia; Nieva, Laura Beatriz Salazar; Acevedo-Barrera, Anays; Mendoza-García, Emma; Garcı́a-Valenzuela, Augusto

doi:10.1088/1361-6501/ab8430

Cited by 10 publications

(6 citation statements)

References 41 publications

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“…In summary, we illustrate in this work the viability of simple and rapid procedure to determine the standard OF curve of blood samples and provides information related to the integrity of the erythrocyte membrane, which can be an indicator of the health status of people associated with different pathologies such as: thalassemia 15,16 , macro or micro spherocytosis 17,18 , and paroxysmal nocturnal hemoglobinuria 19,20 , among others 21 . One of its main advantages over other techniques such as flow cytometry is that it is a rapid and economical test so that it does not require large material resources and can open the way to develop new robust instruments for the early detection of different blood pathologies.…”

Section: Discussionmentioning

confidence: 99%

ErythrocytesÓ? osmotic fragility test with a standard Abbe refractometer

Pérez-Pacheco¹,

Salazar-Nieva²,

Garcı́a-Valenzuela³

et al. 2020

HGMX

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Background:The osmotic fragility (OF) test is one of the most suitable procedures to determine the resistance of the erythrocyte membrane under conditions of osmotic stress. One of the most frequent clinical uses of the FO test is the diagnosis of hereditary conditions that affect erythrocytes: thalassemia and spherocytosis. Both conditions induce red blood cells (RBCs) to be prone to swelling and then lyse under osmotic stress faster than those RBCs without any pathology. Since the standard OF test is time consuming and needs specialized equipment, a fast, accessible, and reliable technique is necessary to determine the OF. Objective: The objective of the study was to evaluate an alternative technique to determine the OF of erythrocytes by refractive index (RI) measurements. The method consists of measuring the real part of the RI of dilutions of whole blood samples with hypotonic solutions at different NaCl concentrations, ranging from 0% to 0.9% using a standard Abbe refractometer. Materials and methods: An experimental study was carried out with volunteers who went to the General Hospital of Mexico "Eduardo Liceaga. " Peripheral blood samples from 12 healthy volunteers were used through the standard blood draw procedure and stored in a heparinized vacutainer tube. Results and conclusions: The proposed technique that uses an Abbe refractometer to measure the infrared of different solutions of whole blood and hypotonic solutions to determine the OF curve has proven to be simpler and more robust than the standard method based on the measurement of light absorption in dilutions of hemolyzed blood. The determination of the OF of erythrocytes rapidly and straightforwardly can be useful in the detection of specific pathologies.

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Section: Discussionmentioning

confidence: 99%

ErythrocytesÓ? osmotic fragility test with a standard Abbe refractometer

Pérez-Pacheco¹,

Salazar-Nieva²,

Garcı́a-Valenzuela³

et al. 2020

HGMX

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“…From the critical angle, the RI of the external medium is derived. Critical angle refractometry by internal reflection of light is more suitable than other techniques for measuring and sensing the refractive index of turbid and/or absorbing media such as colloids and biological tissue (Meeten and North, 1995; Hui and Shusen, 1996; Meeten, 1997; Yoshida et al , 2013; Ding et al , 2005; Ding et al , 2006; García-Valenzuela et al , 2002; Morales-Luna and García-Valenzuela, 2017; Márquez-Islas et al , 2020). Nevertheless, measurements of an RI from transmittance near the critical angle are also possible in some cases (Contreras-Tello and García-Valenzuela, 2014; Meeten and North, 1991).…”

Section: Introductionmentioning

confidence: 99%

Functional refractive-index sensor by internal reflection of diffuse light

Pineda-Vázquez

Garcı́a-Valenzuela²

2022

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Purpose This study aims to propose and study a refractive index sensor based on measuring variations of the internal diffuse reflectance from a glass interface in a functional design. The device is uncomplicated to assemble with simple optical elements and it can be built as a robust and stable sensor. Design/methodology/approach This study presents a simplified theoretical model of the signal obtained with the proposed device and perform a detailed analysis of its potential resolution and merits. Findings The authors report proof-of-principle experiments with a home-made device to evaluate its performance as a refractometer and index of refraction sensor. Originality/value The main novelty of the device is the use of a diffusing surface to couple light into a glass plate with a wide range of angles of refraction, including angles larger than the critical angle with the external medium, and using the same diffusing surface to couple reflected light out of the glass plate, including light that suffered total internal reflection.

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“…Likewise, the reflectivity of a well-collimated beam (e.g. a laser beam) near the critical angle in an internal-reflection configuration at a glass interface can be used as a simple and sensitive means of measuring and monitoring the refractive index of the external medium [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, measurement of the cell's refractive index requires simultaneously measuring the thickness and the refractive index of the intervening thin layer. Some of these works [6,12] demonstrate the practicality and high sensitivity of near-critical-angle reflectometry, leading to exciting new applications.…”

Section: Introductionmentioning

confidence: 99%

Analysis of wavelength-scale 1D depth-dependent refractive-index gradients at an interface by their effects on the internal reflectance near the critical angle

et al. 2021

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Light's internal reflectivity near a critical angle is very sensitive to the angle of incidence and the optical properties of the external medium near the interface. Novel applications in biology and medicine of subcritical internal reflection are being pursued. In many practical situations the refractive index of the external medium may vary with respect to its bulk value due to different physical phenomena at surfaces. Thus, there is a pressing need to understand the effects of a refractiveindex gradient at a surface for near-critical-angle reflection. In this work we investigate theoretically the reflectivity near the critical angle at an interface with glass assuming the external medium has a continuous depthdependent refractive index. We present graphs of the internal reflectivity as a function of the angle of incidence, which exhibit the effects of a refractive-index gradient at the interface. We analyse the behaviour of the reflectivity curves before total internal reflection is achieved. Our results provide insight into how one can recognise the existence of a refractive-index gradient at the interface and shed light on the viability of characterising it.

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Optical device and methodology for optical sensing of hemolysis in hypotonic media

Cited by 10 publications

References 41 publications

ErythrocytesÓ? osmotic fragility test with a standard Abbe refractometer

ErythrocytesÓ? osmotic fragility test with a standard Abbe refractometer

Functional refractive-index sensor by internal reflection of diffuse light

Analysis of wavelength-scale 1D depth-dependent refractive-index gradients at an interface by their effects on the internal reflectance near the critical angle

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