Optical diagnostics of bile duct tissues state with tumor compression

Abstract

The paper presents the results of in vivo experimental measurements of the endogenous fluorescence and blood perfusion in common bile duct tissues in obstructive jaundice patients during minimally invasive surgical operations. Nowadays the type of interventions become more widespread for treating hepatopancreatobiliary pathologies. An effective feedback which can be established by one or more optical diagnostic channels integrated into the standard instruments for minimally invasive manipulations would be of particular value to practical surgeons. To collect reference data, an experimental study has been conducted using a fiber optical probe with fluorescence spectroscopy and laser Doppler flowmetry channels. For every measurement, the areas of interest were different areas of common bile duct obstructed by a malignant tumor. Obtained results demonstrated significant variety registered intensities of fluorescence spectra and values of blood perfusion. Tested fiber optical endoscopic system showed sensitivity to the tissue state in the investigated area, so the two modal approach seems to be promising for further research and implementation in clinical practice.

Optical spectroscopy for in vivo medical diagnosis—a review of the state of the art and future perspectives

“…The most common multimodal optical diagnosis type is FS combined with DRS (FS + DRS) [153,175,191,. The other types are; FS plus RS plus DRS (FS + RS + DRS) [292][293][294], FS plus RS (FS + RS) [295,296], FS plus DRS plus EOSS (FS + DRS + EOSS) [297][298][299][300] and FS combined with Doppler flowmetry (FS + Doppler flowmetry) [301][302][303]. These multimodal spectroscopy systems have been used on many different parts of the anatomy, including the alimentary tract, brain, cervix, endocrine system, head & neck (including oral and nasopharynx), heart and the liver.…”

Testing a Fine-Needle Optical Probe for Recording Changes in the Fluorescence of Coenzymes of Cellular Respiration

“…It combines the advantages of conventional biopsy and compensates its main drawback, that is, the need to remove a tissue sample and spend significant time to analyze it. The optical biopsy includes several spectroscopic and visualization methods [15,16], which yield additional diagnostic in vivo information about various parameters of the morphology and metabolism of biological tissues [17,18]. In optical fine-needle aspiration biopsy using small outer-diameter needles (14-22 G or 0.7-2.1 mm), the development of fine-needle optical probes for implementing various spectroscopic diagnostic methods is the most common field of preclinical studies [19][20][21][22][23][24].…”