Optical diagnostics of vascular reactions triggered by weak allergens using laser speckle-contrast imaging technique

Kuznetsov, Yu. L.; Kalchenko, Vyacheslav; Astaf'eva, N G; Meglinski, Igor

doi:10.1070/qe2014v044n08abeh015489

Cited by 7 publications

(5 citation statements)

References 33 publications

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“…The computational analysis of spatial localisation of the LSCI and FS detected signals [18,19] at 785 nm shows that the effective penetration depth in rodents tissues lies in a range $ 0.5-0.7 mm). Since the thickness of skin and skull in rodents is about $ 0.5 mm, the young rats have been selected for noninvasive transcranial cerebral imaging.…”

Section: Methodsmentioning

confidence: 99%

Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

Piavchenko

Kozlov

Dremin

et al. 2021

Journal of Biophotonics

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The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi‐modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy and diffuse reflectance spectroscopy. The results of laser speckle contrast imaging show a notable reduction of cerebral blood flow in small and medium size vessels during a few minutes of respiratory arrest, while the same effect was observed in large sinuses and their branches during the circulatory cessation. Concurrently, the redox ratio assessed with fluorescence spectroscopy indicates progressing hypoxia, NADH accumulation and increase of FAD consumption. The results of diffuse reflectance spectra measurements display a more rapid grow of the perfusion of deoxygenated blood in case of circulatory impairment. In addition, consequent histopathological analysis performed by using new tissue staining procedure developed in‐house. It shows notably higher reduction of size of the neurons due to their wrinkling within brain tissues influenced by circulation impair. Whereas, the brain tissues altered with the respiratory arrest demonstrate focal perivascular oedema and mild hypoxic changes of neuronal morphology. Thus, the study suggests that consequences of a cessation of cerebral blood flow become more dramatic and dangerous compare to respiratory arrest.

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Section: Methodsmentioning

confidence: 99%

Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

Piavchenko

Kozlov

Dremin

et al. 2021

Journal of Biophotonics

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“…This approach has been also used for observation of blood and lymph micro-flows in tumors [12][13][14], and for visualization of skin vascular network [15] and tumor surroundings [16]. Moreover, it was demonstrated that this approach allows visualization and quantitative assessment of vascular reaction in an immediate response to the optical clearing agents, chemical agents, and potential allergens interaction with the skin [17]. The LSI-FIM system has been extensively used in the routine mouse ear swelling test (MEST) screenings [18].…”

Section: Introductionmentioning

confidence: 99%

A Robust Method for Adjustment of Laser Speckle Contrast Imaging during Transcranial Mouse Brain Visualization

et al. 2019

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Laser speckle imaging (LSI) is a well-known and useful approach for the non-invasive visualization of flows and microcirculation localized in turbid scattering media, including biological tissues (such as brain vasculature, skin capillaries etc.). Despite an extensive use of LSI for brain imaging, the LSI technique has several critical limitations. One of them is associated with inability to resolve a functionality of vessels. This limitation also leads to the systematic error in the quantitative interpretation of values of speckle contrast obtained for different vessel types, such as sagittal sinus, arteries, and veins. Here, utilizing a combined use of LSI and fluorescent intravital microscopy (FIM), we present a simple and robust method to overcome the limitations mentioned above for the LSI approach. The proposed technique provides more relevant, abundant, and valuable information regarding perfusion rate ration between different types of vessels that makes this method highly useful for in vivo brain surgical operations.

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“…Laser speckle contrast imaging (LSCI) is a powerful method mostly used for the non-invasive real-time mapping/diagnosis of flow [13]. LSCI has been successfully implemented for different biomedical applications such as cerebral blood flow monitoring [14,15], vascular biology [16,17], tumor screening [18], the monitoring of weak allergen reactions [19][20][21], clinical use on skin [22], and others. In addition, LSCI is effectively used in industry for paint drying monitoring and roughness measurements [23,24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of handwriting peculiarities utilizing laser speckle contrast imaging

Kuznetsov¹,

Sdobnov²,

Meglinski³

et al. 2019

Laser Phys. Lett.

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Functional handwriting is a process involving various complex interactions between physical, cognitive and sensory systems. Since muscular motion is of a peculiar nature for each person, handwriting properties, such as pencil pressure and speed of writing, can be considered as a unique marker of identity. Moreover, impairments of handwriting in many cases are connected to neurodevelopmental disorders such as attention deficit hyperactivity disorder, developmental coordination disorder, autism spectrum disorders, Parkinson's disease, etc. From this point of view, investigations of handwriting kinematics and pressure can be highly important for both forensic science and medicine. Commonly, the kinematic and pressure features of handwriting are evaluated using a graphics tablet with a stylus or electronic pens. The production of such devices is quite expensive. Therefore, the development of new methods for individual handwriting analysis is an important and current goal. Laser speckle contrast imaging (LSCI) is a powerful method, which is sensitive to both motion and pressure. Since the developed technique requires the use of only a simple laser diode and camera for image acquisition, LSCI is a cost-effective and practical tool for handwriting analysis. In the current letter we present a robust LSCI-based method for handwriting pressure and kinematics evaluation. The introduced approach was validated by an Archimedean spiral writing task.

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Optical diagnostics of vascular reactions triggered by weak allergens using laser speckle-contrast imaging technique

Cited by 7 publications

References 33 publications

Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

Impairments of cerebral blood flow microcirculation in rats brought on by cardiac cessation and respiratory arrest

A Robust Method for Adjustment of Laser Speckle Contrast Imaging during Transcranial Mouse Brain Visualization

Evaluation of handwriting peculiarities utilizing laser speckle contrast imaging

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