Optical DNA Biosensor Based on Square-Planar Ethyl Piperidine Substituted Nickel(II) Salphen Complex for Dengue Virus Detection

Abstract

A sensitive and selective optical DNA biosensor was developed for dengue virus detection based on novel square-planar piperidine side chain-functionalized N,N′-bis-4-(hydroxysalicylidene)-phenylenediamine-nickel(II), which was able to intercalate via nucleobase stacking within DNA and be functionalized as an optical DNA hybridization marker. 3-Aminopropyltriethoxysilane (APTS)-modified porous silica nanospheres (PSiNs), was synthesized with a facile mini-emulsion method to act as a high capacity DNA carrier matrix. The Schiff base salphen complexes-labelled probe to target nucleic acid on the PSiNs renders a colour change of the DNA biosensor to a yellow background colour, which could be quantified via a reflectance transduction method. The reflectometric DNA biosensor demonstrated a wide linear response range to target DNA over the concentration range of 1.0 × 10−16–1.0 × 10−10 M (R2 = 0.9879) with an ultralow limit of detection (LOD) at 0.2 aM. The optical DNA biosensor response was stable and maintainable at 92.8% of its initial response for up to seven days of storage duration with a response time of 90 min. The reflectance DNA biosensor obtained promising recovery values of close to 100% for the detection of spiked synthetic dengue virus serotypes 2 (DENV-2) DNA concentration in non-invasive human samples, indicating the high accuracy of the proposed DNA analytical method for early diagnosis of all potential infectious diseases or pathological genotypes.

A need to raise the bar — A systematic review of temporal trends in diagnostics for Japanese encephalitis virus infection, and perspectives for future research

“…Recently, there have been significant developments in highly sensitive molecular point-of-care tests for flaviviruses, such as reverse transcription loop-mediated isothermal amplification (RT-LAMP), reverse transcription recombinase polymerase amplification (RT-RPA), nucleic acid sequence-based amplification (NASBA), transcription-mediated amplification (TMA), helicase-dependent amplification (HDA), and nicking enzyme amplification reaction (NEAR) (Calvert et al, 2017;Ganguli et al, 2017;Kurosaki et al, 2017;Mauk et al, 2017;Priye et al, 2017;Yaren et al, 2018;Castro et al, 2018;Guo et al, 2018;Kim et al, 2018a;Kumar et al, 2018;Lopez-Jimena et al, 2018;Lamb et al, 2018;Sabalza et al, 2018;Song et al, 2018;Zhao and Feng, 2019;Abd et al, 2017;Chan et al, 2018;Tan et al, 2018;Vasileva Wand et al, 2018;Saa et al, 2018). Microfluidics, chips, paper-based devices, and biosensors are also being developed (Onyango et al, 2017;Adegoke et al, 2017;Afsahi et al, 2018;Ariffin et al, 2018;Wasik et al, 2017).…”

New Tools for Dengue Diagnostics

“…The fiber optic reflectance spectrophotometer was used to measure the reflectance intensity of hybridization of target DNA with probe DNA. The study demonstrated that genosensor was capable to detect dengue DNA with a LOD of 0.2 aM [35].…”

Challenges and Opportunities of Laboratory diagnosis of Dengue virus infection: a review

“…Biosensors utilize different mechanisms namely, electrochemical, optical or piezoelectric transduction and surface plasmon resonance (SPR) to detect viral pathogens [98][99][100]. Application of biosensors to detect dengue virus has been reported in several studies [101][102][103][104][105]. In those studies, a range of biosensor-based diagnostic technologies like surface plasmon resonance (SPR) to detect dengue viruses in the serum, saliva and urine samples using different biomarkers complementary DNA (cDNA), IgM antibody and non-structural protein 1 (NS1) antigen was demonstrated with the promising limit of detections as shown in Table 3.…”