2019
DOI: 10.1117/1.jbo.25.1.014506
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Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems

Abstract: Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems," J.Abstract. The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based m… Show more

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Cited by 4 publications
(8 citation statements)
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“…Lifetimes of 2.5 ns for a saturated solution of coumarin 6 (Sigma-Aldrich) in ethanol, and 2.2 ns for 10 µm YG fluorescent microspheres (Polysciences) were recovered, which agree with reference values. 43,44…”
Section: Resultsmentioning
confidence: 99%
“…Lifetimes of 2.5 ns for a saturated solution of coumarin 6 (Sigma-Aldrich) in ethanol, and 2.2 ns for 10 µm YG fluorescent microspheres (Polysciences) were recovered, which agree with reference values. 43,44…”
Section: Resultsmentioning
confidence: 99%
“…Moreover, controlling excitation polarization gives better control of the system. The emission photon’s spectral position is encoded as a delay using fiber optics [ 33 ]. The spectral resolution can be varied by changing the fiber length, and we found 30 m length suitable for our experiments.…”
Section: Resultsmentioning
confidence: 99%
“…The spectral contrast is derived using an optical fiber (Fujikura GRIN fiber 800 µm-diameter, 30 m-long, Fujikura, Tokyo, Japan) collection of light, which produces a dispersion of input wavelengths [ 33 ]. The dispersion was calibrated for this fiber in the previous publication as 10 nm wavelength for every unit 200 ps shift in time [ 33 ]. The spectral-dispersion has been described elsewhere [ 33 ].…”
Section: Methodsmentioning
confidence: 99%
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