Optical Imaging Fiber-Based Single Live Cell Arrays:  A High-Density Cell Assay Platform

Biran, Iftah; Walt, David R.

doi:10.1021/ac020009e

Cited by 127 publications

(103 citation statements)

References 45 publications

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“…The microarray with multiple microspheres increases the signal to noise ratio and enhances the detection capabilities. In a whole cell biosensor, the fiber optic array enabled the simultaneous monitoring and spatially resolving the fluorescence signals obtained from individual cells [89]. Cellular responses of the cells were monitored by reporter genes or fluorescent indicators.…”

Section: Sensing Schemesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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(ABSTRACT)Fiber-optic Fabry-Perot interferometric (FFPI) sensors have been widely used due to their high sensitivity, ease of fabrication, miniature size, and capability for multiplexing. However, direct measurement of self-assembled thin films, receptor immobilization process or biological reaction is limited in the FFPI technique due to the difficulty of forming Fabry-Perot cavities by the thin film itself. Novel methods are needed to provide an accurate and reliable measurement for monitoring the thin-film growth in the nanometer range and under various conditions. In this work, two types of fiber-optic multicavity Fabry-Perot interferometric (MFPI) sensors with built-in temperature compensation were designed and fabricated for thin-film measurement, with applications in chemical and biological sensing. Both the tubing-based MFPI sensor and microgap MFPI sensor provide simple, yet high performance solutions for thin-film sensing. The temperature dependence of the sensing cavity is compensated by extracting the temperature information from a second multiplexed cavity. This provides the opportunity to examine the thin-film characteristics under different environment temperatures.To demonstrate the potential of this structure for practical applications, immunosensors were fabricated and tested using these structures. Self-assembled polyelectrolytes served as a precursor film for immobilization of antibodies to ensure they retain their biological activity. This not only provides a convenient method for protein immobilization but also presents the possibility of increasing the binding capacity

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Section: Sensing Schemesmentioning

confidence: 99%

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

et al. 2005

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“…Microfabrication techniques commonly used for the construction of BioMEMS devices include silicon micromachining and lithography, chemical etching, laser ablation, photopolymerization, micromolding, and embossing (29,33,56,84,180,245). These processes can be used to create the valves, channels, reservoirs, and other discrete microstructures critical to the function of BioMEMS devices and may also allow the incorporation of sensing or control elements such as microelectrodes or ion-selective field-effect transistors (59).…”

Section: Biological Microelectromechanical Systemsmentioning

confidence: 99%

Single-Cell Microbiology: Tools, Technologies, and Applications

Brehm-Stecher

Johnson

2004

Microbiol Mol Biol Rev

451

316

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The field of microbiology has traditionally been concerned with and focused on studies at the population level. Information on how cells respond to their environment, interact with each other, or undergo complex processes such as cellular differentiation or gene expression has been obtained mostly by inference from population-level data. Individual microorganisms, even those in supposedly “clonal” populations, may differ widely from each other in terms of their genetic composition, physiology, biochemistry, or behavior. This genetic and phenotypic heterogeneity has important practical consequences for a number of human interests, including antibiotic or biocide resistance, the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the potential of pathogens to cause disease. New appreciation of the importance of cellular heterogeneity, coupled with recent advances in technology, has driven the development of new tools and techniques for the study of individual microbial cells. Because observations made at the single-cell level are not subject to the “averaging” effects characteristic of bulk-phase, population-level methods, they offer the unique capacity to observe discrete microbiological phenomena unavailable using traditional approaches. As a result, scientists have been able to characterize microorganisms, their activities, and their interactions at unprecedented levels of detail

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“…It seems currently unrealistic to combine the accuracy and dynamic range of alternative but sequential real-time PCR with the wide parallelism of hybridisation arrays. The combination of the advantageous features of microarrays and RT-PCR might be achieved by transferring RT-PCR in array-like 3-D formats such as nanowell plates with miniaturised wells having diameters between ~ 100 and ~ 500 micrometers [60]. However, novel technologies to measure gene expression with the ability to accurately analyse allele-specific and splice-variant-specific expression profiles at a dramatic cost reduction, and with a throughput in the range of at least 100,000 measurements per day, are essential for basic studies on RNA expression profiling.…”

Section: Transcriptome Analysismentioning

confidence: 99%

Genome Projects and the Functional-Genomic Era

Sauer

Konthur

Lehrach

2005

CCHTS

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The problems we face today in public health as a result of the -fortunately -increasing age of people and the requirements of developing countries create an urgent need for new and innovative approaches in medicine and in agronomics. Genomic and functional genomic approaches have a great potential to at least partially solve these problems in the future. Important progress has been made by procedures to decode genomic information of humans, but also of other key organisms. The basic comprehension of genomic information (and its transfer) should now give us the possibility to pursue the next important step in life science eventually leading to a basic understanding of biological information flow; the elucidation of the function of all genes and correlative products encoded in the genome, as well as the discovery of their interactions in a molecular context and the response to environmental factors. As a result of the sequencing projects, we are now able to ask important questions about sequence variation and can start to comprehensively study the function of expressed genes on different levels such as RNA, protein or the cell in a systematic context including underlying networks. In this article we review and comment on current trends in large-scale systematic biological research. A particular emphasis is put on technology developments that can provide means to accomplish the tasks of future lines of functional genomics.

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Optical Imaging Fiber-Based Single Live Cell Arrays: A High-Density Cell Assay Platform

Cited by 127 publications

References 45 publications

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor

Single-Cell Microbiology: Tools, Technologies, and Applications

Genome Projects and the Functional-Genomic Era

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