Optical immunoassay for snake venom detection

Dong, Le Van; Eng, Khoo Hoon; Quyen, Le Khac; Gopalakrishnakone, P.

doi:10.1016/j.bios.2003.11.020

Cited by 30 publications

(20 citation statements)

References 25 publications

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“…Van Dong et al used horse-peroxidase-induced precipitation of tetramethylbenzidine to detect the presence of beta-bungarotoxin (Van Dong et al, 2002). They also exploited optical changes of gold-coated silicon chips for detecting the same toxin (Van Dong et al, 2004). Selvanayagam et al used changes in the electrical current of field-effect transistor to detect beta-bungarotoxin .…”

Section: Introductionmentioning

confidence: 99%

A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors

Hartono

Lai

Yang

et al. 2009

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors

Hartono

Lai

Yang

et al. 2009

Biosensors and Bioelectronics

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“…More recently a specific and sensitive optical immunoassay (OIA) for venom detection was developed; this was similar to EIA but based on the principle of the detection of physical changes in the thickness of a molecular film resulting from specific binding events on an optical silicon chip [26]. The reflection of white light through the thin film results in destructive interference of the light from gold to purple-blue depending on the thickness of the thin film formed or the amount of venom in the test sample.…”

Section: Biodetection Methods Considered For Use In Venom Researchmentioning

confidence: 99%

Diagnosis of Snakebite and the Importance of Immunological Tests in Venom Research

Theakston

Laing

2014

Toxins

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In many cases of envenoming following snake bite, the snake responsible for the accident remains unidentified; this frequently results in difficulty deciding which antivenom to administer to the systemically-envenomed victim, especially when only monospecific antivenoms are available. Normally the specific diagnosis of snake bite can be conveniently made using clinical and laboratory methods. Where clinical diagnosis depends upon the recognition of specific signs of envenoming in the patient, laboratory diagnosis is based on the changes which occur in envenomed victims including the detection of abnormalities in blood parameters, presence/absence of myoglobinuria, changes in certain enzyme levels, presence/absence of neurotoxic signs and the detection in the blood of specific venom antigens using immunologically-based techniques, such as enzyme immunoassay. It is the latter which is the main subject of this review, together with the application of techniques currently used to objectively assess the effectiveness of new and existing antivenoms, to assess first aid measures, to investigate the possible use of such methods in epidemiological studies, and to detect individual venom components. With this in mind, we have discussed in some detail how such techniques were developed and how they have helped in the treatment of envenoming particularly and in venom research in general.

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“…Enzyme-linked immunosorbent assay (ELISA) is a successful technique used in a number of medical applications (22). ELISA performed on a sample from the bite site is considered the most reliable and effective method for species identification because there are vast variations in venom constituents among different species.…”

Section: Remote Species Identificationmentioning

confidence: 99%

“…Research has indicated that specific monovalent anti-snake venom (ASV) serum is the method of choice (22), but due to the non-availability of standard techniques for species identification, administration of quadrivalent ASV serum catering to all venomous snakes is the only method currently practiced.…”

Section: Venom Interactions and Physiological Manifestations In Indiamentioning

confidence: 99%

Neural network-based species identification in venom-interacted cases in India

Maheshwari¹,

Kumar²,

Verma³

2007

J. Venom. Anim. Toxins incl. Trop. Dis

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ABSTRACT:India is home to a number of venomous species. Every year in harvesting season, a large number of productive citizens are envenomed by such species. For efficient medical management of the victims, identification of the aggressor species as well as assessment of the envenomation degree is necessary.Species identification is generally based on the visual description by the victim or a witness and is therefore quite likely to be erroneous. Symptomatic identification remains the only available method. In a previous published work, the authors proposed a classification table for snake species based on manifested symptoms applicable in Indian subcontinent. The classification table serves the purpose to a great deal but as a manual method it demands human expertise. The current paper presents a neural network-based symptomatic species identification system. A symptom vector is fed as input to the neural network and the system yields the most probable species as well as the envenomation severity as the output. The severity status can be very helpful in calculating the antivenom dosage and in deciding the species-specific prognostic measures for efficient medical management.

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Optical immunoassay for snake venom detection

Cited by 30 publications

References 25 publications

A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors

A liquid crystal-based sensor for real-time and label-free identification of phospholipase-like toxins and their inhibitors

Diagnosis of Snakebite and the Importance of Immunological Tests in Venom Research

Neural network-based species identification in venom-interacted cases in India

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