Optical Label-Free Nanoplasmonic Biosensing Using a Vertical-Cavity Surface-Emitting Laser and Charge-Coupled Device

Hedsten, Karin; Fonollosa, Jordi; Enoksson, Peter; Modh, P.; Bengtsson, Jörgen; Sutherland, Duncan S.; Dmitriev, Alexandre

doi:10.1021/ac9025169

Cited by 11 publications

(5 citation statements)

References 28 publications

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“…By incorporating the nanoplasmonic chip in a flow cell, its biosensing capability was shown by preparing a biotinylated surface and subsequently monitoring the real-time binding of Neutravidin. Furthermore, also implementation of a Vertical-cavity surface-emitting laser (VCSEL)-optical excitation, combined with a CCD camera for detection, significantly reduces the dimensions of the setup [195]. In this work, the biosensing measurements based on the study of biotin-neutravidin interactions, showed similar outcome when compared to the use of a spectrophotometer.…”

Section: Integration Microfluidics and Multiplexingmentioning

confidence: 74%

Trends and challenges of refractometric nanoplasmonic biosensors: A review

Estévez

Otte

Sepúlveda

et al. 2014

Analytica Chimica Acta

284

214

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Motivated by potential benefits such as sensor miniaturization, multiplexing opportunities and higher sensitivities, refractometric nanoplasmonic biosensing has profiled itself in a short time span as an interesting alternative to conventional Surface Plasmon Resonance (SPR) biosensors. This latter conventional sensing concept has been subjected during the last decades to strong commercialization, thereby strongly leaning on well-developed thin-film surface chemistry protocols. Not surprisingly, the examples found in literature based on this sensing concept are generally characterized by extensive analytical studies of relevant clinical and diagnostic problems. In contrast, the more novel Localized Surface Plasmon Resonance (LSPR) alternative finds itself in a much earlier, and especially, more fundamental stage of development. Driven by new fabrication methodologies to create nanostructured substrates, published work typically focuses on the novelty of the presented material, its optical properties and its use - generally limited to a proof-of-concept - as a label-free biosensing scheme. Given the different stages of development both SPR and LSPR sensors find themselves in, it becomes apparent that providing a comparative analysis of both concepts is not a trivial task. Nevertheless, in this review we make an effort to provide an overview that illustrates the progress booked in both fields during the last five years. First, we discuss the most relevant advances in SPR biosensing, including interesting analytical applications, together with different strategies that assure improvements in performance, throughput and/or integration. Subsequently, the remaining part of this work focuses on the use of nanoplasmonic sensors for real label-free biosensing applications. First, we discuss the motivation that serves as a driving force behind this research topic, together with a brief summary that comprises the main fabrication methodologies used in this field. Next, the sensing performance of LSPR sensors is examined by analyzing different parameters that can be invoked in order to quantitatively assess their overall sensing performance. Two aspects are highlighted that turn out to be especially important when trying to maximize their sensing performance, being (1) the targeted functionalization of the electromagnetic hotspots of the nanostructures, and (2) overcoming inherent negative influence that stem from the presence of a high refractive index substrate that supports the nanostructures. Next, although few in numbers, an overview is given of the most exhaustive and diagnostically relevant LSPR sensing assays that have been recently reported in literature, followed by examples that exploit inherent LSPR characteristics in order to create highly integrated and high-throughput optical biosensors. Finally, we discuss a series of considerations that, in our opinion, should be addressed in order to bring the realization of a stand-alone LSPR biosensor with competitive levels of sensitivity, robustness and integration (whe...

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Section: Integration Microfluidics and Multiplexingmentioning

confidence: 74%

Trends and challenges of refractometric nanoplasmonic biosensors: A review

Estévez

Otte

Sepúlveda

et al. 2014

Analytica Chimica Acta

284

214

View full text Add to dashboard Cite

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“…The shift in the resonance position of the gold nanodome arrays in response to a change in the surrounding RI also leads to changes in extinction (or transmission) for each structure at wavelengths around the resonance maximum. Consequently, monitoring the plasmon shifts through the change in extinction at a single wavelength , is a promising method for reducing the size and cost of the required apparatus, as it eliminates the need for a spectrometer and broadband light source. , Figure a shows the change in extinction with wavelength for a sample (with P = 300 nm, H = 150 nm, and t = 40 nm) first immersed in ethanol and then in water. The differential spectra were used to highlight the wavelengths most sensitive to RI change.…”

Section: Resultsmentioning

confidence: 99%

Plasmonic Sensing Using Nanodome Arrays Fabricated by Soft Nanoimprint Lithography

McPhillips¹,

McClatchey²,

Kelly³

et al. 2011

J. Phys. Chem. C

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Arrays of gold-coated nanodomes were fabricated on glass substrates using a soft nanoimprint lithography technique. Optical transmission measurements revealed complex plasmonic resonances that proved highly sensitive to the array dimensions, the thickness of the gold layer, and the refractive index of the surrounding medium. As one promising application for these structures, the refractive index sensing capabilities of the nanodome arrays were assessed.

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“…The instrument does not require complex signal processing or microscopic optics for signal collection. The authors report low femtomoles detection levels of adsorbed proteins …”

Section: Microanalytical Systemsmentioning

confidence: 98%

“…The authors report low femtomoles detection levels of adsorbed proteins. 39 An array of electrodes fabricated on a monolithic quartz wafer was used to create a multichannel monolithic quartz crystal microbalance (MQCM) to miniaturize a MEMS device for highthroughput analysis. Applications for the device include classification and detection of volatile organic compounds (VOCs, i.e., ethanol, CH 2 Cl 2 , hexane) and water.…”

Section: Reviewmentioning

confidence: 99%