One of the most promising options
for decreasing the costs of microalgae
production is enhancing the production and reducing the energy demand
of the culturing systems and the high surface area requirements. Because
microalgae growth requires only specific wavelengths of the solar
spectrum, the remaining part of the solar spectrum may be simultaneously
used by a translucent photovoltaic (PV) layer to produce electricity,
which leads to a reduction of space and energy requirements. This
work presents the results of a new concept of a positive energy culturing
system for microalgae, where the light source is selectively shared
between the needs of the algal biomass through photosynthesis and
the production of PV energy through dye-sensitized solar cells (DSCs).
To ascertain the DSC (DSC-Red, DSC-Green) light-filtering effects
on microalgal biomass, (1) the variation of growth kinetics, (2) microalgae
pigments [chlorophylls-(a + b) and
carotenoids], and (3) macromolecule content (carbohydrates, proteins,
and lipids) were investigated and compared to control cultures under
two different solar-simulated light intensities (200 and 600 W/m2). The results showed a net improvement of the growth rate
and dry weight at the higher irradiance using both colored DSC filters
compared to control cultures. The highest growth rates (μ) and
doubling time (td) of Chlorella vulgaris cells were obtained using the DSC-Red (DSC-R) and DSC-Green (DSC-G)
solar cells as filters with μ = 0.86 ± 0.01 day–1; t
d = 0.80 day and μ = 0.85 ±
0.03 day–1; t
d = 0.81
day, respectively, compared to normal glass control μ = 0.51
± 0.03 day–1; t
d = 1.35 day. A significant increase in the chlorophyll-a content was obtained under low light intensity for both DSC-colored
compared to control culture, and there was no significant variation
in the macromolecule content measured under the tested light intensities.
Finally, a life cycle assessment based on a functional unit of 1 kg
of the produced algal biomass using the DSC–photobioreactor
(DSC–PBR) was performed and compared to a normal glass PBR.
The results were expressed in terms of CO2 emission equivalents
produced and electricity generated. A fraction of electricity generated
by DSC–PBR is used for bubbling, and the extra electricity
is injected into the electricity grid. This resulted in net negative
GHG emissions.