Optical phase conjugation (OPC)-assisted isotropic focusing

Jang, Mooseok; Sentenac, Anne; Yang, Changhuei

doi:10.1364/oe.21.008781

Cited by 20 publications

(8 citation statements)

References 21 publications

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“…Ultrasound wave, SHG active material, and fluorescent bead with a filter all serve as localized coherent light sources inside tissue media that can be time-reversed by the OPC process. The symmetric optical propagation property of OPC further enables the generation of a quasi-isotropic optical focal spot within a scattering medium [18].…”

Section: Introductionmentioning

confidence: 99%

Method for auto-alignment of digital optical phase conjugation systems based on digital propagation

et al. 2014

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Optical phase conjugation (OPC) has enabled many optical applications such as aberration correction and image transmission through fiber. In recent years, implementation of digital optical phase conjugation (DOPC) has opened up the possibility of its use in biomedical optics (e.g. deep-tissue optical focusing) due to its ability to provide greater-than-unity OPC reflectivity (the power ratio of the phase conjugated beam and input beam to the OPC system) and its flexibility to accommodate additional wavefront manipulations. However, the requirement for precise (pixel-topixel matching) alignment of the wavefront sensor and the spatial light modulator (SLM) limits the practical usability of DOPC systems. Here, we report a method for auto-alignment of a DOPC system by which the misalignment between the sensor and the SLM is auto-corrected through digital light propagation. With this method, we were able to accomplish OPC playback with a DOPC system with gross sensor-SLM misalignment by an axial displacement of up to~1.5cm , rotation and tip/tilt of ~5  , and in-plane displacement of ~5mm (dependent on the physical size of the sensor and the SLM). Our auto-alignment method robustly achieved a DOPC playback peak-to-background ratio (PBR) corresponding to more than ~30% of the theoretical maximum. As an additional advantage, the auto-alignment procedure can be easily performed at will and, as such, allows us to correct for small mechanical drifts within the DOPC systems, thus overcoming a previously major DOPC system vulnerability. We believe that this reported method for implementing robust DOPC systems will broaden the practical utility of DOPC systems.

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Section: Introductionmentioning

confidence: 99%

Method for auto-alignment of digital optical phase conjugation systems based on digital propagation

et al. 2014

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“…Furthermore, by combining 4Pi microscopy with a multi‐focal multi‐photon imaging technique, the recording speed can be increased . Combination of this microscopy with an optical phase conjugation system allows alignments simplification, while combination with adaptive optics facilitates the image quality improvement . 4Pi microscopy can also be incorporated into other super‐resolution imaging techniques, yielding setups such as 4Pi‐STED, 4Pi‐SMLM, and 4Pi‐RESOLFT, which provide multi‐color, live‐cell, 3D super‐resolution, real‐time, whole‐cell or SPT imaging for biological applications including characterization of actin cytoskeleton, transcription cycle, endoplasmic reticulum, bacteriophages, mitochondria, nuclear pore complexes, primary cilia, Golgi‐apparatus‐associated COPI vesicles, and mouse spermatocyte synaptonemal complexes .…”

Section: Methods For Axial Srfmmentioning

confidence: 99%

Breaking the Axial Diffraction Limit: A Guide to Axial Super‐Resolution Fluorescence Microscopy

Liu

Toussaint

Okoro

et al. 2018

Laser & Photonics Reviews

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Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic world. However, for centuries its resolving ability remained limited by the optical diffraction limit. Super‐resolution fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly expand the fields in which optical microscopy can be applied. Unfortunately, SRFM contributes little towards axial resolution enhancement, rendering observation of the axial and three‐dimensional structures of biological tissues difficult; this may yield a misunderstanding of intracellular interactions. Based on the existing literature, the development of axial SRFM is still behind that of lateral SRFM. In light of this, this Review presents a comprehensive summary of the principles, development, characteristics, and applications of existing techniques for improving the axial resolution. This Review will provide a guide to researchers and promote further development of related technology.

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“…Before discussing these approaches, it is important to understand how the wavefront-shaping technique 34,40,113 is used to enhance the sensitivity of ultrasound-light interactions. When combined with UOT-based or PAT-based approaches, an optimal wavefront can be obtained to focus light on the ultrasonic guide star through approaches based on iterative optimization 34,57 , optical phase conjugation 114 or a TM 70 . Owing to the reciprocity of light scattering, the optimal wavefront cancels the effect of light scattering in a 'timereversed' manner and is focused back onto the ultrasound focus.…”

Section: Ultrasound-assisted Optical Imagingmentioning

confidence: 99%