Optical properties and biointerface interactions of chitin

Barzic, Andreea Irina; Albu, Raluca Marinica

doi:10.1007/s00289-020-03406-x

Cited by 9 publications

(8 citation statements)

References 42 publications

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“…Thanks to its abundance and extraordinary features, such as biocompatibility and biodegradability, this biopolymer is used in the fields of tissue engineering (TE) [ 9 , 10 ]. It should be stated that chitin also present cohesive interaction with blood components, e.g., erythrocytes, IgG, fibrinogen (beneficial for preventing side-effects), and nontoxicity [ 11 ]. Nevertheless, for creating an effective scaffold, other parameters must also be provided, such as advantageous 3D geometrical conformation, porosity, wettability, surface roughness, and suitable nano- and micromechanical properties [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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Tissue engineering (TE) is a field of regenerative medicine that has been experiencing a special boom in recent years. Among various materials used as components of 3D scaffolds, naturally formed chitinous materials seem to be especially attractive because of their abundance, non-toxic and eco-friendly character. In this study, chitinous skeleton isolated from the marine sponge Aplysina fistularis (phylum: Porifera) was used for the first time as a support for the cultivation of murine fibroblasts (Balb/3T3), human dermal fibroblasts (NHDF), human keratinocyte (HaCaT), and human neuronal (SH-SY5Y) cells. Characterization techniques such as ATR FTIR, TGA, and μCT, clearly indicate that an interconnected macro-porous, thermostable, pure α-chitin scaffold was obtained after alkali–acid treatment of air-dried marine sponge. The biocompatibility of the naturally formed chitin scaffolds was confirmed by cell attachment and proliferation determined by various microscopic methods (e.g., SEM, TEM, digital microscopy) and specific staining. Our observations show that fibroblasts and keratinocytes form clusters on scaffolds that resemble a skin structure, including the occurrence of desmosomes in keratinocyte cells. The results obtained here suggest that the chitinous scaffold from the marine sponge A. fistularis is a promising biomaterial for future research about tissues regeneration.

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Section: Introductionmentioning

confidence: 99%

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

et al. 2021

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“…The color of polymeric materials is an optical property that can intervene in the physical properties of the polymer and is closely related to the structural conformation 21,22 . Polymers that do not present photochromism or atom donating radicals present greater photoprotection capacity or resistance to photooxidation of the polymer chains.…”

Section: Resultsmentioning

confidence: 99%

“…The color of polymeric materials is an optical property that can intervene in the physical properties of the polymer and is closely related to the structural conformation. 21,22 Polymers that do not present photochromism or atom donating radicals present greater photoprotection capacity or resistance to photooxidation of the polymer chains. Likewise, dark or gray tones favor the reflection of light, and consequently present high values of protection of the active agent against UVB radiation, if they are used in encapsulation or coating processes.…”

Section: Evaluation Of the Physicochemical Characteristics Of Polyiso...mentioning

confidence: 99%

Polyisoprenes obtained from jackfruit latex (Artocarpus heterophyllus L.): Extraction and characterization

Ramos‐Martínez

González-Cruz

Calderón‐Santoyo

et al. 2022

J of Applied Polymer Sci

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Jackfruit latex is a residue from the agricultural production of this fruit, which does not have an application in the food or pharmaceutical industry. The objectives of the study were to (1) extract the polyisoprenes from jackfruit latex using the surfactant sodium lauryl sulfate, and (2) characterize their physicochemical and techno-functional properties. The chemical composition, foaming and emulsifying capacity, and thermal behavior. According to the proximal chemical analysis of the extracts, the major content were lipophilic compounds (89%), including polyisoprenes. Regarding the emulsifying properties, the concentrates extracts showed intermediate values for the emulsifying activity index (32-59 m 2 /g) and stability times from 15 to 95 min. The thermal analysis showed endothermic events (50-65 C) associated with the melting of the concentrate, as well as exothermic events related to the crystallization of the polyisoprenes. The FTIR spectra indicates the presence of the polyisoprenes in the extracts, the stretching of the C═C bonds in 1704 at 1712 cm À1 are characteristic of cis-1,4 polyisoprene bonds, and at 980 cm À1 the C H bending. The physicochemical and techno-functional properties of polyisoprene extracts indicate that they could be used in emulsions or electrohydrodynamic encapsulation processes.

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“…However, the limitations resulting from the structural properties of Frontiers in Bioengineering and Biotechnology frontiersin.org chitin, such as its significantly difficult solubility, limit its commercial application. The fundamental work of Barzic and Albu (2021); Ehrlich et al, 2007 shows in detail the cohesive interaction of crustacean chitin with blood components, such as erythrocytes, fibrinogen, blood fluid, albumin (beneficial for preventing side effects), and microbes (like Streptococcus oralis, Escherichia coli, Shigella dysenteriae, Staphylococcus epidermidis, and Actinomyces naeslundii), which proves their bacteriostatic properties. These results confirm the potential biomedical utility of chitin.…”

Section: Discussionmentioning

confidence: 99%

Chitin scaffolds derived from the marine demosponge Aplysina fistularis stimulate the differentiation of dental pulp stem cells

Zawadzka-Knefel,

Rusak,

Mrozowska

et al. 2023

Front. Bioeng. Biotechnol.

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The use of stem cells for tissue regeneration is a prominent trend in regenerative medicine and tissue engineering. In particular, dental pulp stem cells (DPSCs) have garnered considerable attention. When exposed to specific conditions, DPSCs have the ability to differentiate into osteoblasts and odontoblasts. Scaffolds are critical for cell differentiation because they replicate the 3D microenvironment of the niche and enhance cell adhesion, migration, and differentiation. The purpose of this study is to present the biological responses of human DPSCs to a purified 3D chitin scaffold derived from the marine demosponge Aplysina fistularis and modified with hydroxyapatite (HAp). Responses examined included proliferation, adhesion, and differentiation. The control culture consisted of the human osteoblast cell line, hFOB 1.19. Electron microscopy was used to examine the ultrastructure of the cells (transmission electron microscopy) and the surface of the scaffold (scanning electron microscopy). Cell adhesion to the scaffolds was determined by neutral red and crystal violet staining methods. An alkaline phosphatase (ALP) assay was used for assessing osteoblast/odontoblast differentiation. We evaluated the expression of osteogenic marker genes by performing ddPCR for ALP, RUNX2, and SPP1 mRNA expression levels. The results show that the chitin biomaterial provides a favorable environment for DPSC and hFOB 1.19 cell adhesion and supports both cell proliferation and differentiation. The chitin scaffold, especially with HAp modification, isolated from A. fistularis can make a significant contribution to tissue engineering and regenerative medicine.

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Optical properties and biointerface interactions of chitin

Cited by 9 publications

References 42 publications

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

Naturally Formed Chitinous Skeleton Isolated from the Marine Demosponge Aplysina fistularis as a 3D Scaffold for Tissue Engineering

Polyisoprenes obtained from jackfruit latex (Artocarpus heterophyllus L.): Extraction and characterization

Chitin scaffolds derived from the marine demosponge Aplysina fistularis stimulate the differentiation of dental pulp stem cells

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