We investigate the motion of massive charged and spinning test particles around a charged dyonic black hole spacetime surrounded by perfect fluid scalar dark matter field. We obtain the equations of motion and find the expressions for the four-velocity for the case of a charged particle, and fourmomentum components for the case of a spinning particle. The trajectories for various values of electric Qe and magnetic Qm charges are investigated under the influence of dark matter field λ. We find the equations of motion of a spinning particle that follows a non-geodesic trajectory via Lagrangian approach in addition to the charged and non-spinning particles that follow geodesic motion in this set-up. We study in detail the properties of innermost stable circular orbits (ISCOs) in the equatorial plane. The study of ISCOs of a spinning massive particle is done by using the pole-dipole approximation. We show that, in addition to the particle's spin, the dark matter field parameter λ and black hole charges (Qm and Qe) have a significant influence on the ISCOs of spinning particles. It is observed that if the spin is parallel to the total angular momentum J (i.e. S > 0), the ISCO parameters (i.e.rISCO, LISCO and EISCO) of a spinning particle are smaller than those of a non-spinning particle, whereas if the spin is anti-parallel to total angular momentum J (i.e. S < 0), the value of the ISCO parameters is greater than that of the non-spinning particle. We also show that for the corresponding values of spin parameter S, the behaviour of Keplerian angular frequency of ISCO ΩISCO is opposite to that of rISCO, LISCO and EISCO. As an astrophysical application, the study of innermost stable circular orbits (ISCOs) may play a significant role as ISCOs can be used to set the inner edge of the accretion disk around black hole as well as the initial condition of the binary black hole mergers.