Optical recording of action potentials from vertebrate nerve terminals using potentiometric probes provides evidence for sodium and calcium components

Salzberg, Brian M.; Obaid, A. L.; Senseman, David M.; Gainer, Harold

doi:10.1038/306036a0

Cited by 160 publications

(118 citation statements)

References 31 publications

Supporting

Mentioning

115

Contrasting

Order By: Relevance

“…Each slice was attached to the bottom of a plastic chamber with tungsten pins and maintained in Ringer's solution at 37°C. The sections were stained for 10 min with a voltagesensitive merocyanine-rhodanine dye, NK2761 (0.04% in Ringer's solution containing 1% DMSO; Hayashibara Biochemical Laboratories) (Kamino et al, 1981;Salzberg et al, 1983;Obaid et al, 1985). Thereafter, the excess dye was washed out with dye-free Ringer's solution for 90 min to allow the slice to recover.…”

Section: Methodsmentioning

confidence: 99%

Demonstration of a Neural Circuit Critical for Imprinting Behavior in Chicks

Nakamori

Sato²,

Atoji³

et al. 2010

J. Neurosci.

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Demonstration of a Neural Circuit Critical for Imprinting Behavior in Chicks

Nakamori

Sato²,

Atoji³

et al. 2010

J. Neurosci.

View full text Add to dashboard Cite

show abstract

“…It has been suggested that the 45 phototoxicity of styryl dyes can be caused by singlet oxygen, an excited state of O 2 produced by energy transfer from the triplet state of the dye to molecular oxygen. 15,26 We investigated the singlet oxygen yields of the dyes by monitoring the emission from singlet oxygen at 1270 nm following excitation of FM4-64, 50 1b and 2b in air-saturated acetonitrile solutions. None of the dyes gave any significant signal at 1270 nm (compared to Rose Bengal, a standard singlet oxygen sensitiser, Φ = 0.74).…”

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Thiophene-based dyes for probing membranes

López‐Duarte

Chairatana

et al. 2015

Org. Biomol. Chem.

View full text Add to dashboard Cite

We report the synthesis of four new cationic dipolar push-pull dyes, together with an evaluation of their photophysical and photobiological characteristics pertinent for imaging membranes by fluorescence and second harmonic generation. All four dyes consist of an N,N-diethylaniline electron-donor conjugated to a pyridinium electron-acceptor via a thiophene bridge, with either vinylene (-CH=CH-) or ethynylene 10 (-CC-) linking groups, and with either singly-charged or doubly-charged pyridinium terminals. The absorption and fluorescence behavior of these dyes was compared to a commercially available fluorescent membrane stain, the styryl dye FM4-64. The hyperpolarizabilities of all dyes were compared using hyper-Rayleigh scattering at 800 nm. Cellular uptake, localization, toxicity and phototoxicity were evaluated using tissue cell cultures (HeLa, SK-OV-3 and MDA-231). Replacing the central alkene bridge 15 of FM4-64 with a thiophene does not substantially change the absorption, fluorescence or hyperpolarizability, whereas changing the vinylene-links to ethynylenes shifts the absorption and fluorescence to shorter wavelengths, and reduces the hyperpolarizability by about a factor of two. SHG and fluorescence imaging experiments in live cells showed that the doubly-charged thiophene dyes localize in plasma membranes, and exhibit lower internalization rates compared to FM4-64, resulting in 20 less signal from the cell cytosol. At a typical imaging concentration of 1 µM, the doubly-charged dyes showed no significant light or dark toxicity, whereas the singly-charged dyes are phototoxic even at 0.5 µM, and toxic in the dark at concentrations above 5 µM. The doubly-charged dyes showed phototoxicity at concentrations greater than 10 µM, although they do not generate singlet oxygen, indicating that the phototoxicity is type I rather than type II. Our data demonstrate that the doubly-charged thiophene dyes 25 are more effective than FM4-64 as nonlinear optical imaging agents for live cells.

show abstract

“…Such a signal is usually not available. However, because dendritic processes have greater membrane area than cell somata, the voltage-sensitive dye fluorescence signal emphasizes corresponding dendritic activity (Salzberg, 1983;Salzberg et al, 1983;Grinvald et al, 1994) and that the CA1 apical dendrite is a key compartment for integration of excitatory synaptic inputs; therefore, to approximate the changes in fluorescence in these local dendritic compartments, we use patch recordings from the apical dendrite as a calibration signal. For all dendritic recordings, we plotted the change in voltage-sensitive dye fluorescence as a function of the change in membrane potential recorded from the dendrites.…”

Section: Ca1 Circuit Integration Of Afferent Inputsmentioning

confidence: 99%

Hippocampal CA1 Circuitry Dynamically Gates Direct Cortical Inputs Preferentially at Theta Frequencies

Ang

Carlson²,

Coulter³

2005

J. Neurosci.

114

129

View full text Add to dashboard Cite

Hippocampal CA1 pyramidal neurons receive intrahippocampal and extrahipppocampal inputs during theta cycle, whose relative timing and magnitude regulate the probability of CA1 pyramidal cell spiking. Extrahippocampal inputs, giving rise to the primary theta dipole in CA1 stratum lacunosum moleculare, are conveyed by the temporoammonic pathway. The temporoammonic pathway impinging onto the CA1 distal apical dendritic tuft is the most electrotonically distant from the perisomatic region yet is critical in regulating CA1 place cell activity during theta cycles. How does local hippocampal circuitry regulate the integration of this essential, but electrotonically distant, input within the theta period? Using whole-cell somatic recording and voltage-sensitive dye imaging with simultaneous dendritic recording of CA1 pyramidal cell responses, we demonstrate that temporoammonic EPSPs are normally compartmentalized to the apical dendritic tuft by feedforward inhibition. However, when this input is preceded at a one-half theta cycle interval by proximally targeted Schaffer collateral activity, temporoammonic EPSPs propagate to the soma through a joint, codependent mechanism involving activation of Schaffer-specific NMDA receptors and presynaptic inhibition of GABAergic terminals. These afferent interactions, tuned for synaptic inputs arriving one-half theta interval apart, are in turn modulated by feedback inhibition initiated via axon collaterals of pyramidal cells. Therefore, CA1 circuit integration of excitatory inputs endows the CA1 principal cell with a novel property: the ability to function as a temporally specific "AND" gate that provides for sequence-dependent readout of distal inputs.

show abstract

Optical recording of action potentials from vertebrate nerve terminals using potentiometric probes provides evidence for sodium and calcium components

Cited by 160 publications

References 31 publications

Demonstration of a Neural Circuit Critical for Imprinting Behavior in Chicks

Demonstration of a Neural Circuit Critical for Imprinting Behavior in Chicks

Thiophene-based dyes for probing membranes

Hippocampal CA1 Circuitry Dynamically Gates Direct Cortical Inputs Preferentially at Theta Frequencies

Contact Info

Product

Resources

About